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Submit ReviewBovine Serum Albumin
Description: BSA (bovine serum albumin) is a blocking buffer for IHC, ICC, ELISA and Western blotting; used to block non-specific antibody binding. Commonly used in cell culture
Purity: ≥98%
Biological Activity for Bovine Serum Albumin
Bovine Serum Albumin (BSA) is a widely used blocking reagent for use in immunohistochemistry (IHC), immunocytochemistry (ICC), ELISAs and Western blotting. Used to block non-specific binding of antibodies. Bovine Serum Albumin can also be used as a protein concentration standard in Bradford assay for protein quantification. BSA is also used in cell culture.
Technical Data for Bovine Serum Albumin
| MW | ~66 kDa |
| Storage | Store at +4°C |
| Purity | ≥98% |
| CAS Number | 9048-46-8 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Bovine Serum Albumin
| Solubility | Soluble to 100 mg/ml in water |
Product Datasheets for Bovine Serum Albumin
References for Bovine Serum Albumin
References are publications that support the biological activity of the product.
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Citations for Bovine Serum Albumin
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Reviews for Bovine Serum Albumin
Average Rating: 4.5 (Based on 4 Reviews.)
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WB blocking buffer.
By
Anonymous on 05/04/2021
Assay Type: In Vitro
Species: Mink
Cell Line/Tissue: MV-1
I prepared a 3% BSA solution in TBS and used this to block my membranes prior to the addition of primary antibodies as well as my diluent for my secondary antibodies.
Blocking in Westerns.
By
Anna Højer Øster on 02/12/2021
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: Ngn3gfp
in the tc and western blotting
Very good.
By
Anonymous on 03/28/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: smooth muscle cell
We use this product for WB. It works very well.
Western blot.
By
Anonymous on 08/05/2018
Assay Type: In Vitro
Species: Mouse
Used to block PVDF membranes for WB. Perfect performance. Works better than milk