Bovine Serum Albumin

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Description: BSA (bovine serum albumin) is a blocking buffer for IHC, ICC, ELISA and Western blotting; used to block non-specific antibody binding. Commonly used in cell culture
Purity: ≥98%
Citations (9)
Reviews (4)

Biological Activity for Bovine Serum Albumin

Bovine Serum Albumin (BSA) is a widely used blocking reagent for use in immunohistochemistry (IHC), immunocytochemistry (ICC), ELISAs and Western blotting. Used to block non-specific binding of antibodies. Bovine Serum Albumin can also be used as a protein concentration standard in Bradford assay for protein quantification. BSA is also used in cell culture.

Technical Data for Bovine Serum Albumin

MW ~66 kDa
Storage Store at +4°C
Purity ≥98%
CAS Number 9048-46-8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Bovine Serum Albumin

Solubility Soluble to 100 mg/ml in water

Product Datasheets for Bovine Serum Albumin

Certificate of Analysis / Product Datasheet
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References for Bovine Serum Albumin

References are publications that support the biological activity of the product.

If you know of a relevant reference for Bovine Serum Albumin, please let us know.

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9 Citations for Bovine Serum Albumin

Citations are publications that use Tocris products. Selected citations for Bovine Serum Albumin include:

Matthias et al (2021) Mechanosensitive expression of lamellipodin promotes intracellular stiffness, cyclin expression, and cell proliferation. J Cell Sci PMID: 34031693

Matthias et al (2021) Mechanosensitive expression of lamellipodin promotes intracellular stiffness, cyclin expression and cell proliferation. J Cell Sci 134 PMID: 34152388

Hanne C et al (2018) Comparison of two different culture conditions for derivation of early hiPSC. Cell Biol Int 42 1467-1473 PMID: 29603519

Pamela J et al (2022) SPG15 protein deficits are at the crossroads between lysosomal abnormalities, altered lipid metabolism and synaptic dysfunction. Hum Mol Genet 31 2693-2710 PMID: 35313342

Do you know of a great paper that uses Bovine Serum Albumin from Tocris? Please let us know.

Reviews for Bovine Serum Albumin

Average Rating: 4.5 (Based on 4 Reviews.)

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BSA as blocking buffer.
By Anonymous on 03/05/2023
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: A7R5 vSMC

5% blocking buffer for immunoflorescent staining.

5% BSA diluted in PBS. 30 min incubation with blocking buffer at RT

review image

Blocking in Westerns.
By Anna Højer Øster on 02/12/2021
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: Ngn3gfp

in the tc and western blotting

Very good.
By Anonymous on 03/28/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: smooth muscle cell

We use this product for WB. It works very well.

review image

Western blot.
By Anonymous on 08/05/2018
Assay Type: In Vitro
Species: Mouse

Used to block PVDF membranes for WB. Perfect performance. Works better than milk