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Bovine Serum Albumin

Cat. No. 5217 9 Citations 4 Reviews Submit a Review Datasheet / COA / SDS
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Description: BSA (bovine serum albumin) is a blocking buffer for IHC, ICC, ELISA and Western blotting; used to block non-specific antibody binding. Commonly used in cell culture
Purity: ≥98%
Datasheet
Citations (9)
Reviews (4)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for Bovine Serum Albumin

Bovine Serum Albumin (BSA) is a widely used blocking reagent for use in immunohistochemistry (IHC), immunocytochemistry (ICC), ELISAs and Western blotting. Used to block non-specific binding of antibodies. Bovine Serum Albumin can also be used as a protein concentration standard in Bradford assay for protein quantification. BSA is also used in cell culture.

Technical Data for Bovine Serum Albumin

MW ~66 kDa
Storage Store at +4°C
Purity ≥98%
CAS Number 9048-46-8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Bovine Serum Albumin

Solubility Soluble to 100 mg/ml in water

Product Datasheets for Bovine Serum Albumin

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References for Bovine Serum Albumin

References are publications that support the biological activity of the product.

If you know of a relevant reference for Bovine Serum Albumin, please let us know.

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9 Citations for Bovine Serum Albumin

Citations are publications that use Tocris products. Selected citations for Bovine Serum Albumin include:

George P et al (2020) High affinity nanobodies block SARS-CoV-2 spike receptor binding domain interaction with human angiotensin converting enzyme. Sci Rep 10 22370 PMID: 33353972

David et al (2020) Dopamine 2 Receptor Signaling Controls the Daily Burst in Phagocytic Activity in the Mouse Retinal Pigment Epithelium. Invest Ophthalmol Vis Sci 61 10 PMID: 32396631

Serena et al (2023) Automated high-content imaging in iPSC-derived neuronal progenitors. SLAS Discov 28 42-51 PMID: 36610640

Glover et al (2023) The developmental basis of fingerprint pattern formation and variation Cell 186 1 PMID: 36764291

Do you know of a great paper that uses Bovine Serum Albumin from Tocris? Please let us know.

Reviews for Bovine Serum Albumin

Average Rating: 4.5 (Based on 4 Reviews.)

5 Star
75%
4 Star
0%
3 Star
25%
2 Star
0%
1 Star
0%

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BSA as blocking buffer.
By Anonymous on 03/05/2023
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: A7R5 vSMC

5% blocking buffer for immunoflorescent staining.

5% BSA diluted in PBS. 30 min incubation with blocking buffer at RT

review image
Blocking in Westerns.
By Anna Højer Øster on 02/12/2021
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: Ngn3gfp

in the tc and western blotting

Very good.
By Anonymous on 03/28/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: smooth muscle cell

We use this product for WB. It works very well.

review image
Western blot.
By Anonymous on 08/05/2018
Assay Type: In Vitro
Species: Mouse

Used to block PVDF membranes for WB. Perfect performance. Works better than milk