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Cat.No. 5216 - BIS-TRIS | C8H19NO5 | CAS No. 6976-37-0
Description: Commonly used Zwitterionic buffer
Chemical Name: Bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane
Purity: ≥99%
Citations (1)
Reviews (1)

Biological Activity

Commonly used zwitterionic buffer. Working pH range in aqueous solution: 5.8 - 7.2. Used in eletrophoresis protocols.

Technical Data

M. Wt 209.24
Formula C8H19NO5
Storage Store at RT
Purity ≥99%
CAS Number 6976-37-0
PubChem ID 81462

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
water 418.48 2000

Preparing Stock Solutions

The following data is based on the product molecular weight 209.24. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
20 mM 0.24 mL 1.19 mL 2.39 mL
100 mM 0.05 mL 0.24 mL 0.48 mL
200 mM 0.02 mL 0.12 mL 0.24 mL
1000 mM 0 mL 0.02 mL 0.05 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Keywords: BIS-TRIS, BIS-TRIS supplier, BIS-TRIS, buffer, zwitterionic, buffering, agent, electrophoresis, Buffers,, Solvents, and, Solutions, 5216, Tocris Bioscience

1 Citation for BIS-TRIS

Citations are publications that use Tocris products. Selected citations for BIS-TRIS include:

Cassé et al (2012) Glutamate controls tPA recycling by astrocytes, which in turn influences glutamatergic signals. J Neurosci 32 5186 PMID: 22496564

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Media ingredient.
By Anonymous on 05/01/2018
Species: Mouse

Media additive