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Description: Biotin with PEG linker and reactive click handle
Chemical Name: (3aS,4S,6aR)-N-[2-[2-[2-(2-Azidoethoxy)ethoxy]ethoxy]ethyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide
Purity: ≥95% (HPLC)

Biological Activity for Biotin-PEG3-Azide

Biotin-PEG3-Azide is a biotinylation reagent for labeling alkyne-containing biomolecules either via Cu(I)-catalyzed Azide-Alkyne Click Chemistry reaction (CuAAC) or via Cu(I)-free Strain-Promoted Alkyne-Azide Click Chemistry (SPAAC) reaction with cyclooctyne derivatives.

Technical Data for Biotin-PEG3-Azide

M. Wt 444.55
Formula C18H32N6O5S
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 875770-34-6
PubChem ID 60146223
Smiles C(CCCC(NCCOCCOCCOCCN=[N+]=[N-])=O)[C@H]1[C@@]2([C@](CS1)(NC(=O)N2)[H])[H]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Biotin-PEG3-Azide

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 44.45 100
water 44.45 100

Preparing Stock Solutions for Biotin-PEG3-Azide

The following data is based on the product molecular weight 444.55. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.25 mL 11.25 mL 22.49 mL
5 mM 0.45 mL 2.25 mL 4.5 mL
10 mM 0.22 mL 1.12 mL 2.25 mL
50 mM 0.04 mL 0.22 mL 0.45 mL

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Product Datasheets for Biotin-PEG3-Azide

References for Biotin-PEG3-Azide

References are publications that support the biological activity of the product.

Zhang et al (2021) Use of NAD tagSeq II to identify growth phase-dependent alterations in E. coli RNA NAD+ capping. Proc.Natl.Acad.Sci.U.S.A. 118 e2026183118 PMID: 33782135

If you know of a relevant reference for Biotin-PEG3-Azide, please let us know.

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Keywords: Biotin-PEG3-Azide, Biotin-PEG3-Azide supplier, Biotin, PEG3, Azide, Click, Chemistry, biotinylation, labeling, labelling, copper, free, CuAAC, Cu, catalyzed, Alkyne, SPAAC, cyclooctyne, Biochemicals, and, Molecular, Biology, 7524, Tocris Bioscience

Citations for Biotin-PEG3-Azide

Citations are publications that use Tocris products.

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