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Description: Biotinylated-NAD+; substrate for ADP-ribosylation
Chemical Name: β-Nicotinamide-N6-[2-[[6-[biotinyl]amino]hexyl]amino]-2-oxoethyl]adenine dinucleotide
Citations (1)

Biological Activity for Biotin-NAD+

Biotin-NAD+ is a provides a convenient non-isotopic alternative to radiolabeled NAD+ for determination of IC50 values for candidate PARP inhibitors and studies requiring this substrate. Biotinylated-NAD+ allows an indirect measure of PARP activity when biotin incorporation is detected using a conjugated-streptavidin detection system. Acts as a substrate for ADP-ribosylation. Can be used to label and purify biotinyl-ADP ribosylated proteins.

This product is a replacement for R&D Systems product 4670-500-01.

131 μg is supplied as 500 μl of a 0.25 mM solution in water.

Technical Data for Biotin-NAD+

M. Wt 1045.95
Formula C39H57N11O17P2S
Storage Store at -80°C
CAS Number 146385-37-7
Smiles [H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCCCCCNC(CNC3=C(N=CN4[C@@H]5O[C@@H]([C@H]([C@H]5O)O)COP(O)(OP(OC[C@H]6O[C@@H]([N+]7=CC=CC(C(N)=O)=C7)[C@H](O)[C@@H]6O)([O-])=O)=O)C4=NC=N3)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Biotin-NAD+

Solubility Soluble in water (supplied pre-dissolved at a concentration of 0.25mM)

Product Datasheets for Biotin-NAD+

Certificate of Analysis / Product Datasheet
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References for Biotin-NAD+

References are publications that support the biological activity of the product.

Zhang et al (1993) Purification of a nitric oxide-stimulated ADP-ribosylated protein using biotinylated beta-nicotinamide adenine dinucleotide. Biochemistry 32 2228 PMID: 8443164

Yang et al (2017) Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9 Mol. Cell 66 613 PMID: 28525742

Schuster et al (2017) The Hsp90 machinery facilitates the transport of diphtheria toxin into human cells. Sci.Rep. 7 613 PMID: 28377614

Yang (2013) Antitumor activity of a pyrrole-imidazole polyamide. Proc.Natl.Acad.Sci.USA 110 1863 PMID: 23319609

If you know of a relevant reference for Biotin-NAD+, please let us know.

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Keywords: Biotin-NAD+, Biotin-NAD+ supplier, 6-Biotin-17-NAD, 6-Biotin-10-NAD, Biotin-NAD+, Biotinylated-NAD, ADP-ribosylation, substrates, PARP, Biochemicals, and, Molecular, Biology, 6573, Tocris Bioscience

1 Citation for Biotin-NAD+

Citations are publications that use Tocris products. Selected citations for Biotin-NAD+ include:

Andrew et al (2022) Neuralized-like protein 4 (NEURL4) mediates ADP-ribosylation of mitochondrial proteins. J Cell Biol 221 PMID: 35157000

Do you know of a great paper that uses Biotin-NAD+ from Tocris? Please let us know.

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