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Cat.No. 4099 - Azathioprine | C9H7N7O2S | CAS No. 446-86-6
Description: Purine analog; prodrug of 6-mercaptopurine (Cat. No. 4103)
Chemical Name: 6-[(1-Methyl-4-nitro-1H-imidazol-5-yl)thio]-9H-purine
Purity: ≥99% (HPLC)
Reviews (1)
Literature (1)

Biological Activity for Azathioprine

Azathioprine is a purine analog. Blocks DNA replication and the de novo pathway of purine synthesis. Immunosuppressive and anti-inflammatory drug; induces apoptosis in human T-lymphocytes. Prodrug of 6-mercaptopurine (Cat. No. 4103).

Compound Libraries for Azathioprine

Azathioprine is also offered as part of the Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data for Azathioprine

M. Wt 277.26
Formula C9H7N7O2S
Storage Store at -20°C
Purity ≥99% (HPLC)
CAS Number 446-86-6
PubChem ID 2265
Smiles CN1C=NC([N+]([O-])=O)=C1SC2=C3C(N=CN3)=NC=N2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Azathioprine

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 27.73 100

Preparing Stock Solutions for Azathioprine

The following data is based on the product molecular weight 277.26. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.61 mL 18.03 mL 36.07 mL
5 mM 0.72 mL 3.61 mL 7.21 mL
10 mM 0.36 mL 1.8 mL 3.61 mL
50 mM 0.07 mL 0.36 mL 0.72 mL

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References for Azathioprine

References are publications that support the biological activity of the product.

Maltzman and Koretzky (2003) Azathioprine: old drug, new actions. J.Clin.Invest. 111 1122 PMID: 12697731

If you know of a relevant reference for Azathioprine, please let us know.

Keywords: Azathioprine, Azathioprine supplier, Azathioprine, immunosuppressant, inhibits, DNA, synthesis, inhibitors, lymphocyte, purine, analog, DNA,, RNA, and, Protein, Synthesis, Immunosuppressants, 4099, Tocris Bioscience

⚠ WARNING: This product can expose you to chemicals including Azathioprine, which is known to the State of California to cause cancer. For more information, go to

Citations for Azathioprine

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Currently there are no citations for Azathioprine. Do you know of a great paper that uses Azathioprine from Tocris? Please let us know.

Reviews for Azathioprine

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Medium concentration of azathioprine slows growth but does not kill melanoma cells in vitro.
By Anonymous on 02/14/2019
Assay Type: In Vitro
Species: Human

Azathioprine was used at a 20 uM concentration for in vitro culture with SK-MEL-28 and cell viability was monitored over time with live imaging. Arrow indicates when compound was added to media.

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