API-1

Pricing Availability   Qty
Description: Selective Akt/PKB inhibitor. Antitumor
Chemical Name: 4-Amino-5,8-dihydro-5-oxo-8-β-D-ribofuranosyl-pyrido[2,3-d]pyrimidine-6-carboxamide
Purity: ≥96% (HPLC)
Datasheet
Citations (4)
Reviews (1)
Pathways (1)

Biological Activity for API-1

API-1 is an Akt/protein kinase B (PKB) inhibitor. Binds the pleckstrin homology domain of Akt and blocks Akt membrane translocation. Inhibits EGF-induced kinase activity of Akt1, Akt2 and Akt3. Induces cell growth arrest and apoptosis in human cancer cells expressing constitutively active Akt. Displays antitumor activity in vitro and in vivo.

Technical Data for API-1

M. Wt 337.29
Formula C13H15N5O6
Storage Store at RT
Purity ≥96% (HPLC)
CAS Number 36707-00-3
PubChem ID 24773090
InChI Key SPBWHPXCWJLQRU-FITJORAGSA-N
Smiles O[C@@H]1[C@@H](CO)O[C@@H](N2C(N=CN=C3N)=C3C(C(C(N)=O)=C2)=O)[C@@H]1O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for API-1

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 33.73 100

Preparing Stock Solutions for API-1

The following data is based on the product molecular weight 337.29. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.96 mL 14.82 mL 29.65 mL
5 mM 0.59 mL 2.96 mL 5.93 mL
10 mM 0.3 mL 1.48 mL 2.96 mL
50 mM 0.06 mL 0.3 mL 0.59 mL

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Product Datasheets for API-1

Certificate of Analysis / Product Datasheet
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References for API-1

References are publications that support the biological activity of the product.

Kim et al (2010) A small molecule inhibits Akt through direct binding to Akt and preventing Akt membrane translocation. J.Biol.Chem. 285 8383 PMID: 20068047


If you know of a relevant reference for API-1, please let us know.

View Related Products by Product Action

View all Akt (Protein Kinase B) Inhibitors

Keywords: API-1, API-1 supplier, protein, kinase, b, pkb, akt, inhibitors, inhibits, antitumor, antitumour, Akt, (Protein, Kinase, B), Protein, B/Akt, 3897, Tocris Bioscience

4 Citations for API-1

Citations are publications that use Tocris products. Selected citations for API-1 include:

Zhu et al (2012) BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells. EMBO J 31 31 PMID: 22463935

Arslan and Yerer (2018) α-Chaconine and α-Solanine Inhibit RL95-2 Endometrium Cancer Cell Proliferation by Reducing Expression of Akt (Ser473) and ERα (Ser167). Nutrients 10 PMID: 29799481

Shamblott et al (2016) Neurogenin 3 is regulated by neurotrophic tyrosine kinase receptor type 2(TRKB) signaling in the adult human exocrine pancreas. Cell Communication and Signaling 14 23 PMID: 27659207

Jezierska-Drutel et al (2019) The peroxidase PRDX1 inhibits the activated phenotype in mammary fibroblasts through regulating c-Jun N-terminal kinases. BMC Cancer 19 812 PMID: 31419957


Do you know of a great paper that uses API-1 from Tocris? Please let us know.

Reviews for API-1

Average Rating: 5 (Based on 1 Review.)

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Study of API-1 induction c-FLIP degradation and synergization with TRAIL to augment apoptosis.
By Anonymous on 01/16/2020
Species: Human

Study of API-1 induction c-FLIP degradation and synergization with TRAIL to augment apoptosis.API-1 effectively induced apoptosis in tested cancer cell lines including activation of caspase-8 and caspase-9.

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Pathways for API-1

Akt Signaling Pathway

Akt Signaling Pathway

The Akt signaling pathway plays a key role in the mediation of protein synthesis, metabolism, proliferation and cell cycle progression. It may be referred to as a 'prosurvival' pathway.