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API-1

Cat. No. 3897 4 Citations 1 Review Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Selective Akt/PKB inhibitor. Antitumor
Chemical Name: 4-Amino-5,8-dihydro-5-oxo-8-β-D-ribofuranosyl-pyrido[2,3-d]pyrimidine-6-carboxamide
Purity: ≥96% (HPLC)
Datasheet
Citations (4)
Reviews (1)
Literature (1)
Pathways (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for API-1

API-1 is an Akt/protein kinase B (PKB) inhibitor. Binds the pleckstrin homology domain of Akt and blocks Akt membrane translocation. Inhibits EGF-induced kinase activity of Akt1, Akt2 and Akt3. Induces cell growth arrest and apoptosis in human cancer cells expressing constitutively active Akt. Displays antitumor activity in vitro and in vivo.

Technical Data for API-1

M. Wt 337.29
Formula C13H15N5O6
Storage Store at RT
Purity ≥96% (HPLC)
CAS Number 36707-00-3
PubChem ID 24773090
InChI Key SPBWHPXCWJLQRU-FITJORAGSA-N
Smiles O[C@@H]1[C@@H](CO)O[C@@H](N2C(N=CN=C3N)=C3C(C(C(N)=O)=C2)=O)[C@@H]1O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for API-1

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 33.73 100

Preparing Stock Solutions for API-1

The following data is based on the product molecular weight 337.29. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.96 mL 14.82 mL 29.65 mL
5 mM 0.59 mL 2.96 mL 5.93 mL
10 mM 0.3 mL 1.48 mL 2.96 mL
50 mM 0.06 mL 0.3 mL 0.59 mL

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Product Datasheets for API-1

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References for API-1

References are publications that support the biological activity of the product.

Kim et al (2010) A small molecule inhibits Akt through direct binding to Akt and preventing Akt membrane translocation. J.Biol.Chem. 285 8383 PMID: 20068047

If you know of a relevant reference for API-1, please let us know.

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Keywords: API-1, API-1 supplier, protein, kinase, b, pkb, akt, inhibitors, inhibits, antitumor, antitumour, Akt, (Protein, Kinase, B), Protein, B/Akt, 3897, Tocris Bioscience

4 Citations for API-1

Citations are publications that use Tocris products. Selected citations for API-1 include:

Jezierska-Drutel et al (2019) The peroxidase PRDX1 inhibits the activated phenotype in mammary fibroblasts through regulating c-Jun N-terminal kinases. BMC Cancer 19 812 PMID: 31419957

Zhu et al (2012) BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells. EMBO J 31 31 PMID: 22463935

Arslan and Yerer (2018) α-Chaconine and α-Solanine Inhibit RL95-2 Endometrium Cancer Cell Proliferation by Reducing Expression of Akt (Ser473) and ERα (Ser167). Nutrients 10 PMID: 29799481

Shamblott et al (2016) Neurogenin 3 is regulated by neurotrophic tyrosine kinase receptor type 2(TRKB) signaling in the adult human exocrine pancreas. Cell Communication and Signaling 14 23 PMID: 27659207

Do you know of a great paper that uses API-1 from Tocris? Please let us know.

Reviews for API-1

Average Rating: 5 (Based on 1 Review.)

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Study of API-1 induction c-FLIP degradation and synergization with TRAIL to augment apoptosis.
By Anonymous on 01/16/2020
Species: Human

Study of API-1 induction c-FLIP degradation and synergization with TRAIL to augment apoptosis.API-1 effectively induced apoptosis in tested cancer cell lines including activation of caspase-8 and caspase-9.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Cancer Research Product Guide

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis

Pathways for API-1

Akt Signaling Pathway

Akt Signaling Pathway

The Akt signaling pathway plays a key role in the mediation of protein synthesis, metabolism, proliferation and cell cycle progression. It may be referred to as a 'prosurvival' pathway.