API-1

Pricing Availability Delivery Time Qty
Cat.No. 3897 - API-1 | C13H15N5O6 | CAS No. 36707-00-3
Description: Selective Akt/PKB inhibitor. Antitumor
Chemical Name: 4-Amino-5,8-dihydro-5-oxo-8-β-D-ribofuranosyl-pyrido[2,3-d]pyrimidine-6-carboxamide
Purity: ≥96% (HPLC)
Datasheet
Citations (2)

Biological Activity

Akt/protein kinase B (PKB) inhibitor. Binds the pleckstrin homology domain of Akt and blocks Akt membrane translocation. Inhibits EGF-induced kinase activity of Akt1, Akt2 and Akt3. Induces cell growth arrest and apoptosis in human cancer cells expressing constitutively active Akt. Displays antitumor activity in vitro and in vivo.

Compound Libraries

API-1 is also offered as part of the Tocriscreen Plus and Tocriscreen Kinase Inhibitor Toolbox I. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 337.29
Formula C13H15N5O6
Storage Store at RT
Purity ≥96% (HPLC)
CAS Number 36707-00-3
PubChem ID 24773090
InChI Key SPBWHPXCWJLQRU-FITJORAGSA-N
Smiles O[C@@H]1[C@@H](CO)O[C@@H](N2C(N=CN=C3N)=C3C(C(C(N)=O)=C2)=O)[C@@H]1O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 33.73 100

Preparing Stock Solutions

The following data is based on the product molecular weight 337.29. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.96 mL 14.82 mL 29.65 mL
5 mM 0.59 mL 2.96 mL 5.93 mL
10 mM 0.3 mL 1.48 mL 2.96 mL
50 mM 0.06 mL 0.3 mL 0.59 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Certificate of Analysis / Product Datasheet
Select another batch:
Safety Datasheet

References

References are publications that support the products' biological activity.

Kim et al (2010) A small molecule inhibits Akt through direct binding to Akt and preventing Akt membrane translocation. J.Biol.Chem. 285 8383 PMID: 20068047


If you know of a relevant reference for API-1, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Akt (Protein Kinase B) Inhibitors

Keywords: protein kinase b pkb akt inhibitors inhibits antitumor antitumour Akt (Protein Kinase B)

2 Citations for API-1

Citations are publications that use Tocris products. Selected citations for API-1 include:

Zhu et al (2012) BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells. EMBO J 31 31 PMID: 22463935

Shamblott et al (2016) Neurogenin 3 is regulated by neurotrophic tyrosine kinase receptor type 2(TRKB) signaling in the adult human exocrine pancreas. Cell Communication and Signaling 14 23 PMID: 27659207


Do you know of a great paper that uses API-1 from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

Pathways for API-1

Protocols

TODO: Add Protocols