Amitriptyline hydrochloride

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Cat.No. 4456 - Amitriptyline hydrochloride | C20H23N.HCl | CAS No. 549-18-8
Description: 5-HT and noradrenalin re-uptake inhibitor; also TrkA/B agonist
Chemical Name: 3-(10,11-Dihydro-5H-dibenzo[a,d]cyclohepten-5-ylidene)-N,N-dimethyl-1-propanamine hydrochloride
Purity: ≥99% (HPLC)

Biological Activity

Inhibitor of serotonin and noradrenaline uptake; also activates α2A-adrenoceptors and antagonizes 5-HT2 receptors. Displays antinociceptive activity. Tricyclic antidepressant (TCA). Also a TrkA/B agonist. Induces neurite outgrowth in PC12 cells.

Technical Data

M. Wt 313.86
Formula C20H23N.HCl
Storage Desiccate at RT
Purity ≥99% (HPLC)
CAS Number 549-18-8
PubChem ID 11065
Smiles CN(CC\C=C2/C3=C(C=CC=C3)CCC1=CC=CC=C12)C.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 31.39 100
ethanol 31.39 100
water 31.39 100

Preparing Stock Solutions

The following data is based on the product molecular weight 313.86. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.19 mL 15.93 mL 31.86 mL
5 mM 0.64 mL 3.19 mL 6.37 mL
10 mM 0.32 mL 1.59 mL 3.19 mL
50 mM 0.06 mL 0.32 mL 0.64 mL

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Product Datasheets

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References are publications that support the products' biological activity.

Merck Index 12 85 PMID:

Sanchez and Hyttel (1999) Comparison of the effects of antidepressants and their metabolites on reuptake of biogenic amines and on receptor binding. Cell.Mol.Neurobiol. 19 467 PMID: 10379421

Ghelardini et al (2000) Antinociception induced by amitriptyline and imipramine is mediated by α2A-adrenoceptors. Jpn.J.Pharmacol. 82 130 PMID: 10877531

Sawynok et al (2001) Antidepressants as analgesics: an overview of central and peripheral mechanisms of action. J.Psychiatry Neurosci. 26 21 PMID: 11212590

If you know of a relevant reference for Amitriptyline hydrochloride, please let us know.

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Keywords: serotonin serotonergics 5-HT noradrenalin noradrenaline net sert reuptake inhibitors inhibits tca tricyclic antidepressants neurotrophic factors TRKA B tropomyosin related kinases agonists agonism 5-HT Transporters

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