Pricing Availability   Qty
Description: Photo-crosslinking probe for studying glycoproteins and glycolipids
Chemical Name: 2-Deoxy-2-[[3-(3-methyl-3H-diazirin-3-yl)-1-oxopropyl]amino]-D-mannopyranose-1,3,4,6-tetraacetate
Purity: ≥98% (HPLC)

Biological Activity for Ac4ManNDAz

Ac4ManNDAz is a cell-permeable photo-crosslinking probe for studying glycoprotein or glycolipid interactions. The compound is an unnatural diazirine-containing monosaccharide building block, which acts as a photo-crosslinker. Once inside the cell, Ac4ManNDAz is metabolized and incorporated into cell surface glycoproteins as a photo-crosslinking sialic acid analog. Upon UV irradiation, the diazirine group is activated to a reactive carbene that covalently crosslinks to nearby molecules.

Technical Data for Ac4ManNDAz

M. Wt 457.44
Formula C19H27N3O10
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1015698-39-1
PubChem ID 122400676
Smiles CC(OC[C@H]1OC([C@H]([C@H]([C@@H]1OC(C)=O)OC(C)=O)NC(CCC2(N=N2)C)=O)OC(C)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Ac4ManNDAz

Solvent Max Conc. mg/mL Max Conc. mM
ethanol 45.74 100
DMSO 45.74 100

Preparing Stock Solutions for Ac4ManNDAz

The following data is based on the product molecular weight 457.44. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.19 mL 10.93 mL 21.86 mL
5 mM 0.44 mL 2.19 mL 4.37 mL
10 mM 0.22 mL 1.09 mL 2.19 mL
50 mM 0.04 mL 0.22 mL 0.44 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

References for Ac4ManNDAz

References are publications that support the biological activity of the product.

Tanaka et al (2008) Photoactivatable crosslinking sugars for capturing glycoprotein interactions. J.Am.Chem.Soc. 130 3278 PMID: 18293988

Bond et al (2009) Photocrosslinking of glycoconjugates using metabolically incorporated diazirine-containing sugars. Nat.Protoc. 4 1044 PMID: 19536272

Bond et al (2011) Metabolism of diazirine-modified N-acetylmannosamine analogues to photo-cross-linking sialosides. Bioconjug.Chem. 22 1811 PMID: 21838313

Cervin et al (2018) GM1 ganglioside-independent intoxication by Cholera toxin. PLoS.Pathog. 14 e1006862 PMID: 29432456

If you know of a relevant reference for Ac4ManNDAz, please let us know.

Keywords: Ac4ManNDAz, Ac4ManNDAz supplier, cell-permeable, unnatural, diazirine, containing, monosaccharide, building, block, photoactivate, photoactivatable, cross-linker, cross-linking, sialic, acids, analog, glycoproteins, glycolipids, probes, probe, Post-translational, Modifications, Biochemicals, and, Molecular, Biology, Glycobiology, Probes, 7553, Tocris Bioscience

Citations for Ac4ManNDAz

Citations are publications that use Tocris products.

Currently there are no citations for Ac4ManNDAz. Do you know of a great paper that uses Ac4ManNDAz from Tocris? Please let us know.

Reviews for Ac4ManNDAz

There are currently no reviews for this product. Be the first to review Ac4ManNDAz and earn rewards!

Have you used Ac4ManNDAz?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review