8-pCPT-2-O-Me-cAMP-AM
Biological Activity
Selective Epac activator; cAMP analog. Induces Rap activation and junction tightening in HUVECs; triggers adhesion of Jurkat-Epac1 cells to fibronectin. Stimulates insulin secretion in rat INS-1 cells. More potent, cell-permeable analog of 8CPT-2Me-cAMP (Cat. No. 1645).
This product is a mixture of axial and equatorial isomers. Both isomers give 8CPT-2Me-cAMP after esterase cleavage.
Licensing Information
Sold with the permission of BioLog Life Science Institute.
Technical Data
| M. Wt | 557.9 |
| Formula | C20H21ClN5O8PS |
| Storage | Store at -80°C |
| Purity | ≥97% (HPLC) |
| CAS Number | 1152197-23-3 |
| PubChem ID | 90488978 |
| InChI Key | FZMWUFYPEVDWPA-SILPBKOMSA-N |
| Smiles | NC1=NC=NC2=C1N=C(SC4=CC=C(Cl)C=C4)N2[C@@H]3O[C@@](COP(O5)(OCOC(C)=O)=O)([H])[C@]5([H])[C@H]3OC |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 55.79 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 557.9. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.79 mL | 8.96 mL | 17.92 mL |
| 5 mM | 0.36 mL | 1.79 mL | 3.58 mL |
| 10 mM | 0.18 mL | 0.9 mL | 1.79 mL |
| 50 mM | 0.04 mL | 0.18 mL | 0.36 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Vliem et al (2008) 8-pCPT-2'-O-Me-cAMP-AM: an improved Epac-selective cAMP analogue. Chembiochem. 9 2052 PMID: 18633951
Almahariq et al (2013) A novel EPAC-specific inhibitor suppresses pancreatic cancer cell migration and invasion. Mol.Pharmacol. 83 122 PMID: 23066090
Tsalkova et al (2012) Isoform-specific antagonists of exchange proteins directly activated by cAMP. Proc.Natl.Acad.Sci.U.S.A. 109 18613 PMID: 23091014
Chepurny et al (2009) Enhanced Rap1 activation and insulin secretagogue properties of an acetoxymethyl ester of an Epac-selective cyclic AMP analog in rat INS-1 cells: studies with 8-pCPT-2'-O-Me-cAMP-AM. J.Biol.Chem. 284 10728 PMID: 19244230
If you know of a relevant reference for 8-pCPT-2-O-Me-cAMP-AM, please let us know.
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Keywords: 8-pCPT-2-O-Me-cAMP-AM, supplier, 007-AM, cAMP, analogs, Epac, epacs, activators, cell, permeable, selective, Rap, 007-AM, GTPase, Tocris Bioscience
1 Citation for 8-pCPT-2-O-Me-cAMP-AM
Citations are publications that use Tocris products. Selected citations for 8-pCPT-2-O-Me-cAMP-AM include:
Watson et al (2015) Endothelial CD99 signals through soluble adenylyl cyclase and PKA to regulate leukocyte transendothelial migration. Mol Brain 212 1021 PMID: 26101266
Do you know of a great paper that uses 8-pCPT-2-O-Me-cAMP-AM from Tocris? If so please let us know.
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