8-CPT-2Me-cAMP, sodium salt

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Cat.No. 1645 - 8-CPT-2Me-cAMP, sodium salt | C17H16ClN5O6PS.Na | CAS No. 634207-53-7
Description: Selective Epac activator
Chemical Name: 8-(4-Chlorophenylthio)-2'-O-methyladenosine-3',5'-cyclic monophosphate sodium salt
Purity: ≥99% (HPLC)
Datasheet
Citations (10)
Reviews
Literature

Biological Activity

Selective activator of Epac, the cAMP-sensitive guanine nucleotide-exchange factor for Rap1 and Rap2. Activates Epac1 (EC50 = 2.2 μM), but not PKA (EC50 > 10 μM). Stimulates Epac-mediated Ca2+-mediated Ca2+ release in pancreatic β-cells in vitro. Cell permeable analog (Cat. No. 4853) also available.

Licensing Information

Sold with the permission of BioLog Life Science Institute

Technical Data

M. Wt 507.82
Formula C17H16ClN5O6PS.Na
Storage Desiccate at -20°C
Purity ≥99% (HPLC)
CAS Number 634207-53-7
PubChem ID 16759604
InChI Key VHTMKTNUXDACHB-KHXPSBENSA-M
Smiles [Na+].[H][C@@]12COP([O-])(=O)O[C@@]1([H])[C@@H](OC)[C@@H](O2)N1C(SC2=CC=C(Cl)C=C2)=NC2=C1N=CN=C2N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 50.78 100

Preparing Stock Solutions

The following data is based on the product molecular weight 507.82. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.97 mL 9.85 mL 19.69 mL
5 mM 0.39 mL 1.97 mL 3.94 mL
10 mM 0.2 mL 0.98 mL 1.97 mL
50 mM 0.04 mL 0.2 mL 0.39 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the biological activity of the product.

Enserink et al (2002) A novel Epac-specific cAMP analogue demonstrates independent regulation of Rap1 and ERK. Nat.Cell.Biol. 4 901 PMID: 12402047

Kang et al (2003) Epac-selective cAMP analog 8-pCPT-2'-O-Me-cAMP as a stimulus for Ca2+-induced Ca2+ release and exocytosis in pancreatic βcells. J.Biol.Chem. 278 8279 PMID: 12496249

Kawasaki et al (1998) A family of cAMP-binding proteins that directly activate Rap1. Science 282 2275 PMID: 9856955


If you know of a relevant reference for 8-CPT-2Me-cAMP, sodium salt, please let us know.

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Keywords: 8-CPT-2Me-cAMP, sodium salt, 8-CPT-2Me-cAMP, sodium salt supplier, Selective, Epacs, epac, activators, G, Proteins, Small, Rap1, Rap2, GTPase, (Small), 1645, Tocris Bioscience

10 Citations for 8-CPT-2Me-cAMP, sodium salt

Citations are publications that use Tocris products. Selected citations for 8-CPT-2Me-cAMP, sodium salt include:

Garcia-Murillas et al (2014) An siRNA screen identifies the GNAS locus as a driver in 20q amplified breast cancer. Oncogene 33 2478 PMID: 23752180

Soltoff and Hedden (2010) Isoproterenol and cAMP block ERK phosphorylation and enhance [Ca2+]i increases and oxygen consumption by muscarinic receptor stimulation in rat parotid and submandibular acinar cells. J Biol Chem 285 13337 PMID: 20207737

Mohan et al (2015) Neuroprotective role of prostaglandin PGE2 EP2 receptor in hemin-mediated toxicity. Endocrinology 46 53 PMID: 25451967

Hu et al (2012) Natriuretic peptides block synaptic transmission by activating phosphodiesterase 2A and reducing presynaptic PKA activity. Proc Natl Acad Sci U S A 109 17681 PMID: 23045693

Himer et al (2010) Adenosine A2A receptor activation protects CD4+ T lymphocytes against activation-induced cell death. FASEB J 24 2631 PMID: 20371613

Scheibner et al (2009) The adenosine a2a receptor inhibits matrix-induced inflammation in a novel fashion. Am J Respir Cell Mol Biol 40 251 PMID: 18703794

Guichard et al (2013) Cholera toxin disrupts barrier function by inhibiting exocyst-mediated trafficking of host proteins to intestinal cell junctions. Cell Host Microbe 14 294 PMID: 24034615

Hutchison and White (2015) Prostacyclin regulates bone growth via the Epac/Rap1 pathway. PLoS One 156 499 PMID: 25406016

Meitzen et al (2011) β1-Adrenergic receptors activate two distinct signaling pathways in striatal neurons. J Neurochem 116 984 PMID: 21143600

Jeong et al (2007) IQGAP1 binds Rap1 and modulates its activity. J Biol Chem 282 20752 PMID: 17517894


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