Pricing Availability   Qty
Description: Potent and selective Nav1.6 channel blocker
Alternative Names: 4,9-anhydro-TTX
Chemical Name: (4S,5aS,6S,8R,9S,10S,11S,11aR,12R)-2-Amino-1,4,5a,6,8,9,10,11-octahydro-9-(hydroxymethyl)-6,10-epoxy-4,8,11a-metheno-11aH-oxocino[4,3-f][1,3,5]oxadiazepine-6,9,11-triol
Purity: ≥98% (HPLC)
Citations (4)

Biological Activity for 4,9-Anhydrotetrodotoxin

4,9-Anhydrotetrodotoxin is a potent and selective Nav1.6 channel blocker (IC50 values are 7.8, 341, 988, 1260, 1270, 78500 and >30000 nM for Nav1.6, Nav1.3, Nav1.4, Nav1.2, Nav1.7, Nav1.5 and Nav1.8). Derivative of tetrodotoxin (Cat. No. 1078).

Technical Data for 4,9-Anhydrotetrodotoxin

M. Wt 301.25
Formula C11H15N3O7
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 13072-89-4
PubChem ID 11243537
Smiles O[C@@]1(CO)[C@H](O2)[C@H]([C@H](O3)NC(N4[H])=N)[C@]54[C@H]3[C@@]2(O)O[C@H]1[C@H]5O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for 4,9-Anhydrotetrodotoxin

Solvent Max Conc. mg/mL Max Conc. mM
water 1 4

Preparing Stock Solutions for 4,9-Anhydrotetrodotoxin

The following data is based on the product molecular weight 301.25. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.04 mM 82.99 mL 414.94 mL 829.88 mL
0.2 mM 16.6 mL 82.99 mL 165.98 mL
0.4 mM 8.3 mL 41.49 mL 82.99 mL
2 mM 1.66 mL 8.3 mL 16.6 mL

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Product Datasheets for 4,9-Anhydrotetrodotoxin

Certificate of Analysis / Product Datasheet
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References for 4,9-Anhydrotetrodotoxin

References are publications that support the biological activity of the product.

Rosker et al (2007) The TTX metabolite 4,9-anhydro-TTX is a highly specific blocker of the Nav1.6 voltage-dependent sodium channel. Am.J.Physiol.Cell Physiol. 293 C783 PMID: 17522141

Hargus et al (2013) Evidence for a role of Nav1.6 in facilitating increases in neuronal hyperexcitability during epileptogenesis J.Neurophysiol 110 1144 PMID: 23741036

If you know of a relevant reference for 4,9-Anhydrotetrodotoxin, please let us know.

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Keywords: 4,9-Anhydrotetrodotoxin, 4,9-Anhydrotetrodotoxin supplier, Na+, channel, blockers, sodium, Nav, 1.6, channels, voltage-gated, voltage-dependent, potent, selective, ttx, 4,9-anhydro-TTX, Voltage-gated, Sodium, Channels, 6159, Tocris Bioscience

4 Citations for 4,9-Anhydrotetrodotoxin

Citations are publications that use Tocris products. Selected citations for 4,9-Anhydrotetrodotoxin include:

Mohammed A et al (2020) An unbiased and efficient assessment of excitability of sensory neurons for analgesic drug discovery. Pain 161 1100-1108 PMID: 31929383

Vysokov et al (2019) The role of NaV channels in synaptic transmission after axotomy in a microfluidic culture platform. Sci Rep 9 12915 PMID: 31501450

Ellen A et al (2019) Tetrodotoxin-Sensitive Sodium Channels Mediate Action Potential Firing and Excitability in Menthol-Sensitive Vglut3-Lineage Sensory Neurons. J Neurosci 39 7086-7101 PMID: 31300524

Darik A et al (2022) NaV1.1 is essential for proprioceptive signaling and motor behaviors. Elife 11 PMID: 36278870

Do you know of a great paper that uses 4,9-Anhydrotetrodotoxin from Tocris? Please let us know.

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