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Description: Class III PI 3-kinase inhibitor; also inhibits autophagy
Alternative Names: 3-MA
Chemical Name: 3-Methyl-3H-purin-6-amine
Purity: ≥99% (HPLC)
Citations (10)

Biological Activity for 3-Methyladenine

3-Methyladenine is an inhibitor of class III phosphatidylinositol 3-kinase (PI 3-kinase); also inhibits the autophagic sequestration of cell proteins in rat hepatocytes. Blocks apoptosis in cerebellar granule cells (CGCs) following serum and potassium deprivation.

Compound Libraries for 3-Methyladenine

3-Methyladenine is also offered as part of the Tocriscreen Kinase Inhibitor Library, Tocriscreen Antiviral Library and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for 3-Methyladenine

M. Wt 149.15
Formula C6H7N5
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 5142-23-4
PubChem ID 1673
Smiles NC(N=CN2C)=C1C2=NC=N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for 3-Methyladenine

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 2.98 20

Preparing Stock Solutions for 3-Methyladenine

The following data is based on the product molecular weight 149.15. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 33.52 mL 167.62 mL 335.23 mL
1 mM 6.7 mL 33.52 mL 67.05 mL
2 mM 3.35 mL 16.76 mL 33.52 mL
10 mM 0.67 mL 3.35 mL 6.7 mL

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Product Datasheets for 3-Methyladenine

Certificate of Analysis / Product Datasheet
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References for 3-Methyladenine

References are publications that support the biological activity of the product.

Blommaart et al (1997) The phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 inhibit autophagy in isolated rat hepatocytes. Eur.J.Biochem. 243 240 PMID: 9030745

Canu et al (2005) Role of the autophagic-lysosomal system on low potassium-induced apoptosis in cultured cerebellar granule cells. J.Neurochem. 92 1228 PMID: 15715672

Seglen and Gordon (1982) 3-methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes. Proc.Natl.Acad.Sci.U.S.A. 79 1889 PMID: 6952238

If you know of a relevant reference for 3-Methyladenine, please let us know.

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Keywords: 3-Methyladenine, 3-Methyladenine supplier, pi, 3-kinase, phosphatidylinositol, 3, kinase, inhibitors, inhibits, autophagy, 3-MA, pi3k, vps34, PIK3C3, PI, 3-Kinase, Inositol, Lipids, Other, Apoptosis, Autophagy, 3977, Tocris Bioscience

10 Citations for 3-Methyladenine

Citations are publications that use Tocris products. Selected citations for 3-Methyladenine include:

Nepal et al (2015) Autophagy induction by leptin contributes to suppression of apoptosis in cancer cells and xenograft model: involvement of p53/FoxO3A axis. Evid Based Complement Alternat Med 6 7166 PMID: 25704884

Kim et al (2013) Epigallocatechin gallate (EGCG) stimulates autophagy in vascular endothelial cells: a potential role for reducing lipid accumulation. J Biol Chem 288 22693 PMID: 23754277

Wang et al (2013) Active Constituents from Liriope platyphylla Root against Cancer Growth In Vitro. Nat Commun 2013 857929 PMID: 23762164

Wolf et al (2013) Miz1 is required to maintain autophagic flux. Clin Transl Med 4 2535 PMID: 24088869

Do you know of a great paper that uses 3-Methyladenine from Tocris? Please let us know.

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