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Unnatural amino acid that induces red shift when incorporated into fluorescent proteins and fluorescent protein-based biosensors. Causes spontaneous and efficient red shift of GFP in HEK293T cells, shifting excitation and emission maxima by 56 and 95 nm respectively. Induces red shift in a range of fluorescent proteins containing tyrosine-derived chromophores, including teal fluorescent mTFP1, yellow fluorescent Citrine, and circularly permuted YFP and GFP. Also induces red-shift in fluorescent protein-based biosensors, including iGluSnFR (a glutamate sensor), iGABASnFR (a GABA sensor), dLight1.2 (a dopamine sensor) and iATPSnFR1.1 (an ATP sensor).
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 269.12. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.72 mL||18.58 mL||37.16 mL|
|5 mM||0.74 mL||3.72 mL||7.43 mL|
|10 mM||0.37 mL||1.86 mL||3.72 mL|
|50 mM||0.07 mL||0.37 mL||0.74 mL|
References are publications that support the biological activity of the product.
Zhang & Ai (2020) A general strategy to red-shift green fluorescent protein-based biosensors. Nat.Chem.Biol. 16 1434 PMID: 32929278
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Keywords: 3-Aminotyrosine, 3-Aminotyrosine supplier, red, shift, fluorescent, protein, based, biosensors, GFP, YFP, TFP, noncanonical, unnatural, amino, acids, UAA, Unnatural, Amino, Acids, 7370, Tocris Bioscience
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