Janelia Fluor™ 549, SE
Cat. No. 6147
Alternative Name: JF 549, NHS
Chemical Name: 3,6-Di-1-azetidinyl-9-[2-carboxy-5-
Biological ActivityFluorescent dye; supplied as an NHS ester for coupling to primary amine groups. Compatible with self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169. Janelia Fluor 646 SE (Cat.No. 6148) also available.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Licensing InformationSold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Custom Conjugation ServiceOur sister Bio-Techne brand Novus Biologicals, offers a custom conjugation service for Janelia FluorTM dyes, for more details please visit the Novus Custom Conjugation Service.
Image shown created using Janelia Fluor™ dyes, this single-cell image is an overlay of a diffusion map of Tetracycline Repressor Protein (green), which leads to antibiotic resistance, and a super-resolution image of histone H2B (purple). Please visit www.janelia.org for more information.
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Certificate of Analysis / Product Datasheet / Safety Datasheet
References are publications that support the products' biological activity.
Legant et al (2016) High-density three-dimensional localization microscopy across large volumes. Nat.Methods 13 359. PMID: 26950745.
Deng et al (2015) CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells. Proc.Natl.Acad.Sci.USA. 112 11870. PMID: 26324940.
Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244. PMID: 25599551.
Knight et al (2015) Dynamics of CRISPR-Cas9 genome interrogation in living cells. Science 350 823. PMID: 26564855.
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April 22 - 26, 2017
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