Acridine Orange hydrochloride

Cat. No. 5092

Acridine Orange hydrochloride C17H19N3.HCl [65-61-2]

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Chemical Name: N,N,N',N'-Tetramethyl-3,6-acridinediamine hydrochloride

Biological Activity

Cell and organelle membrane permeable nucleic acid binding dye. Emits green fluorescence when bound to double stranded DNA and red fluorescence when bound to RNA or single stranded DNA. Used in cell cycle and apoptosis studies and as a lysosomal dye.

Technical Data

M.Wt:
301.81
Formula:
C17H19N3.HCl
Solubility:
Soluble to 100 mM in water and to 100 mM in DMSO
Purity:
>99 %
Storage:
Store at RT
CAS No:
65-61-2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Product Datasheet / Safety Datasheet

References are publications that support the products' biological activity.

Kiyoshima et al (2013) Chemoresistance to concanamycin A1 in human oral squamous cell carcinoma is attenuated by an HDAC inhibitor partly via suppression of Bcl-2 expression. PLoS ONE 8 80998. PMID: 24278362.

Ratan et al (2008) Rapid communication: oxidative stress induces apoptosis in embryonic cortical neurons. J.Neurobiol. 62 376. PMID: 7903353.

McMaster et al (1977) Analysis of single and double stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc.Natl.Acad.Sci.USA 74 4835. PMID: 73185.

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Keywords: Acridine Orange hydrochloride, supplier, nucleic, acid, binding, dyes, cell, cycle, lysosomal, DNA, stains, RNA, Tocris Bioscience, Fluorescent Dye products

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