PF 573228

Cat. No. 3239

PF 573228 C22H20F3N5O3S [869288-64-2]

Price and Availability

For PF 573228 pricing & availability please select your country from the drop down menu:
By clicking submit you agree to accept a cookie from Tocris Bioscience. For details, please read our privacy and cookie policy.

Chemical Name: 3,4-Dihydro-6-[[4-[[[3-(methylsulfonyl)phenyl]methyl]amino]-5-(trifluoromethyl)-2-pyrimidinyl]amino]-2(1H)-quinolinone

Biological Activity

Potent and selective inhibitor of focal adhesion kinase (FAK) (IC50 = 4 nM). Displays 50 - 250-fold selectivity for FAK over other protein kinases. Blocks serum and fibronectin-directed migration and decreases focal adhesion turnover in vitro.

Licensing Information

Sold for research purposes under agreement from Pfizer Inc.

Technical Data

Soluble to 100 mM in DMSO
>98 %
Store at +4°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.

Certificate of Analysis / Product Datasheet / Safety Datasheet

References are publications that support the products' biological activity.

Kostourou et al (2013) FAK-heterozygous mice display enhanced tumour angiogenesis. Nat.Commun. 5 2020. PMID: 23799510.

Slack-Davis et al (2007) Cellular characterization of a novel focal adhesion kinase inhibitor. J.Biol.Chem. 282 14845. PMID: 17395594.


If you know of a relevant reference for PF 573228 please let us know.

Citations are publications that use Tocris products. Selected citations for PF 573228 include:

Song (2017) Disruption of outer blood-retinal barrier by Toxoplasma gondii-infected monocytes is mediated by paracrinely activated FAK signaling PLoS One 12 (4) e0175159. PMID: 28406972.

Alowayed et al (2016) LEFTY2 Controls Migration of Human Endometrial Cancer Cells via Focal Adhesion Kinase Activity (FAK) and miRNA-200a Cell Physiol Biochem 39 815. PMID: 27497669.

Hennigan et al (2016) Eosinophil peroxidase activates cells by HER2 receptor engagement and β1-integrin clustering with downstream MAPK cell signaling Clinical Immunology 171 1. PMID: 27519953.

Nasrollahi and Pathak (2016) Topographic confinement of epithelial clusters induces epithelial-to-mesenchymal transition in compliant matrices. PLoS One 6 18831. PMID: 26728047.

Rengarajan et al (2016) Endothelial Cells Use a Formin-Dependent Phagocytosis-Like Process to Internalize the Bacterium Listeria monocytogenes. Sci Rep 12 e1005603. PMID: 27152864.

Carrim et al (2015) Thrombin-induced reactive oxygen species generation in platelets: A novel role for protease-activated receptor 4 and GPIbα. J Exp Med 6 640. PMID: 26569550.

Cheng et al (2015) IgE-activated basophils regulate eosinophil tissue entry by modulating endothelial function. Sci Rep 212 513. PMID: 25779634.

Gu et al (2015) Defective apical extrusion signaling contributes to aggressive tumor hallmarks. Elife 4 e04069. PMID: 25621765.

Webber et al (2015) Combination of heat shock protein 90 and focal adhesion kinase inhibitors synergistically inhibits the growth of non-small cell lung cancer cells. Oncoscience 2 765. PMID: 26501082.

Zhang et al (2015) GABAB receptor upregulates fragile X mental retardation protein expression in neurons. Redox Biol 5 10468. PMID: 26020477.

Alao et al (2014) Selective inhibition of RET mediated cell proliferation in vitro by the kinase inhibitor SPP86. BMC Cancer 14 853. PMID: 25409876.

Flannagan et al (2014) The phosphatidylserine receptor TIM4 utilizes integrins as coreceptors to effect phagocytosis. Mol Biol Cell 25 1511. PMID: 24623723.

Shih et al (2014) Astroglial-derived periostin promotes axonal regeneration after spinal cord injury. J Biol Chem 34 2438. PMID: 24523534.

Wrighton et al (2014) Signals from the surface modulate differentiation of human pluripotent stem cells through glycosaminoglycans and integrins. PLoS Pathog 111 18126. PMID: 25422477.

Chapman et al (2013) Focal adhesion kinase negatively regulates Lck function downstream of the T cell antigen receptor. J Immunol 191 6208. PMID: 24227778.

Garrison et al (2013) Reversal of myofibroblast differentiation by prostaglandin E(2). Mol Cancer Ther 48 550. PMID: 23470625.

Keasey et al (2013) Inhibition of a novel specific neuroglial integrin signaling pathway increases STAT3-mediated CNTF expression. Cell Commun Signal 11 35. PMID: 23693126.

Quint et al (2013) Sphingosine 1-phosphate (S1P) receptors 1 and 2 coordinately induce mesenchymal cell migration through S1P activation of complementary kinase pathways. PLoS One 288 5398. PMID: 23300082.

Wiel et al (2013) Lysyl oxidase activity regulates oncogenic stress response and tumorigenesis. Cell Death Dis 4 e855. PMID: 24113189.

Yamashita et al (2013) Periostin links mechanical strain to inflammation in abdominal aortic aneurysm. J Neurosci 8 e79753. PMID: 24260297.

Beauséjour et al (2012) Integrin/Fak/Src-mediated regulation of cell survival and anoikis in human intestinal epithelial crypt cells: selective engagement and roles of PI3-K isoform complexes. Apoptosis 17 566. PMID: 22402981.

Chu et al (2012) Contractile Activity Regulates Inducible Nitric Oxide Synthase Expression and NO(i) Production in Cardiomyocytes via a FAK-Dependent Signaling Pathway. J Signal Transduct 2012 473410. PMID: 22900166.

Guessous et al (2012) Cooperation between c-Met and focal adhesion kinase family members in medulloblastoma and implications for therapy. Mol Cancer 11 288. PMID: 22188814.

Wong et al (2012) Focal adhesion kinase links mechanical force to skin fibrosis via inflammatory signaling. Am J Respir Cell Mol Biol 18 148. PMID: 22157678.

Canonici et al (2011) Saccharomyces boulardii improves intestinal cell restitution through activation of the α2β1 integrin collagen receptor. Hepatology 6 e18427. PMID: 21483797.

Daley et al (2011) A focal adhesion protein-based mechanochemical checkpoint regulates cleft progression during branching morphogenesis. Dev Dyn 240 2069. PMID: 22016182.

Schrader et al (2011) Matrix stiffness modulates proliferation, chemotherapeutic response, and dormancy in hepatocellular carcinoma cells. Biochem Biophys Res Commun 53 1192. PMID: 21442631.

Hao et al (2010) tPA is a potent mitogen for renal interstitial fibroblasts: role of beta1 integrin/focal adhesion kinase signaling. Am J Pathol 177 1164. PMID: 20639453.

Ocak et al (2010) DNA copy number aberrations in small-cell lung cancer reveal activation of the focal adhesion pathway. Oncogene 29 6331. PMID: 20802517.

Tu et al (2010) GABAB receptor activation protects neurons from apoptosis via IGF-1 receptor transactivation. J Neurosci 30 749. PMID: 20071540.

Jones et al (2009) Characterization of a novel focal adhesion kinase inhibitor in human platelets. Nat Med 389 198. PMID: 19716803.

Do you know of a great paper that uses PF 573228 from Tocris? If so please let us all ▼show less ▲

View Related Products by Product Action

Keywords: PF 573228, supplier, Potent, selective, FAK, inhibitors, inhibits, Focal, Adhesion, Kinases, PF573228, Tocris Bioscience, Focal Adhesion Kinase Inhibitor products

Quick Order

Find multiple products by catalog number

divider line

Cancer Research Product Guide

Cancer Research Product Guide

Our Cancer Research Guide highlights over 750 products for cancer research. Request copy or view PDF today.

divider line

Kinase Inhibitor Toolbox

80 Kinase inhibitors
pre-dissolved in DMSO

Kinase Inhibitor Toolbox

Includes many of the latest kinase inhibitors to become commercially available. Covers a wide range of kinase targets including EGFR, MAPK and CDK.

divider line

Kinases Product Listing

Kinases Product Listing

Our Kinase listing highlights over 400 products for kinase research. Request copy or view PDF today.

divider line

New Products in this Area

HS 38

ATP-competitive inhibitor of DAPK and ZIPK

st-Ht31 P

Negative control for st-Ht31 (Cat. No. 6286)


Inhibits PKA/AKAP interactions; cell permeable

P21d hydrochloride

Potent and selective Brk inhibitor

CVT 313

cdk2 inhibitor


High affinity PKC activator; also APP modulator

JH 295

Irreversible Nek2 inhibitor


Potent Syk kinase inhibitor; also inhibits assembly of NLRP3 inflammasomes


Serine/threonine protein kinase 16 (STK16) inhibitor; also inhibits PI 3Kδ, PI 3Kγ and mTOR kinases.


Potent and selective PFKFB3 inhibitor

NCL 00017509

Potent and reversible NIMA related kinase 2 (Nek2) inhibitor

XMD 8-87

Potent Ack1/TNK2 inhibitor

SKI 178

Non-lipid sphingosine kinase 1 (SphK1) inhibitor

ML 315 hydrochloride

Inhibitor of Clk and DYRK kinases


mTOR inhibitor;immunosuppressant

PKI (5-24)

High affinity PKA inhibitor


Selective non-ATP competitive inhibitor of GSK 3β

BI 605906

Selective IKKβ inhibitor

GSK 319347A

Potent and selective IKKε inhibitor

FRAX 597

Potent group I PAK inhibitor

MRT 68921 dihydrochloride

Potent ULK inhibitor; inhibits autophagy

AM 2394

Potent glucokinase activator; orally bioavailable

eCF 309

Potent mTOR inhibitor

PF 3758309 dihydrochloride

Potent PAK4 inhibitor; orally available

AZ Dyrk1B 33

Potent and selective Dyrk1B kinase inhibitor

Sign-up for new product e-alerts
divider line

Bio-Techne Events


6th Annual Meeting GDR3545

November 8 - 10, 2017

Paris, France