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Cell-permeable, irreversible pan-caspase inhibitor. Inhibits caspase processing and apoptosis induction in tumor cells in vitro (IC50 = 0.0015 - 5.8 mM). Active in vivo.
(Modifications: Val-1 = Z-Val, Asp-3 = (OMe)-fluoromethylketone)
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solubility||Soluble to 9.35 mg/ml in DMSO|
References are publications that support the biological activity of the product.
Slee et al (1996) Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32. Biochem.J. 315 21 PMID: 8670109
Kunstle et al (1997) ICE-protease inhibitors block murine liver injury and apoptosis caused by CD95 or by TNF-α. Immunol.Lett. 55 5 PMID: 9093874
Garcia-Calvo et al (1998) Inhibition of human caspases by peptide-based and macromolecular inhibitors. J.Biol.Chem. 273 32608 PMID: 9829999
King et al (1998) Processing/activation of caspases, -3 and -7 and -8 but not caspase-2, in the induction of apoptosis in B-chronic lyphocytic leukemia cells. Leukemia 12 1553 PMID: 9766499
If you know of a relevant reference for Z-VAD-FMK, please let us know.
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Keywords: Z-VAD-FMK, Z-VAD-FMK supplier, Cell-permeable, irreversible, caspases, inhibitors, inhibits, Proteinases, Proteases, Caspases, Inflammasomes, 2163, Tocris Bioscience
32 Citations for Z-VAD-FMK
Citations are publications that use Tocris products. Selected citations for Z-VAD-FMK include:
Vago et al (2012) Annexin A1 modulates natural and glucocorticoid-induced resolution of inflammation by enhancing neutrophil apoptosis. J Leukoc Biol 92 249 PMID: 22493082
Moon et al (2015) mTORC1-Induced HK1-Dependent Glycolysis Regulates NLRP3 Inflammasome Activation. Cell Rep 12 102 PMID: 26119735
Im-aram et al (2013) The mTORC2 component rictor contributes to cisp. resistance in human ovarian cancer cells. PLoS One 8 e75455 PMID: 24086535
Jahchan et al (2013) A drug repositioning approach identifies tricyclic antidepressants as inhibitors of small cell lung cancer and other neuroendocrine tumors. Cancer Discov 3 1364 PMID: 24078773
Kim et al (2013) A ginseng metabolite, compound K, induces autophagy and apoptosis via generation of reactive oxygen species and activation of JNK in human colon cancer cells. Cell Death Dis 4 e750 PMID: 23907464
Uderhardt et al (2019) Resident Macrophages Cloak Tissue Microlesions to Prevent Neutrophil-Driven Inflammatory Damage. Cell 177 541 PMID: 30955887
Bae et al (2019) Circulating Cell-Free mtDNA Contributes to AIM2 Inflammasome-Mediated Chronic Inflammation in Patients with Type 2 Diabetes. Cells 8 PMID: 30965677
Bagnjuk et al (2019) Necroptosis in primate luteolysis: a role for ceramide. Cell Death Discov 5 67 PMID: 30774995
Kuang et al (2015) Guadecitabine (SGI-110) priming sensitizes hepatocellular carcinoma cells to oxalip. Mol Oncol 9 1799 PMID: 26160429
Sullivan et al (2015) Human ACAP2 is a homolog of C. elegans CNT-1 that promotes apoptosis in cancer cells. Cell Cycle 14 1771 PMID: 25853217
Hight et al (2014) A peptide-based positron emission tomography probe for in vivo detection of caspase activity in apoptotic cells. Evid Based Complement Alternat Med 20 2126 PMID: 24573549
Wang et al (2013) Active Constituents from Liriope platyphylla Root against Cancer Growth In Vitro. Mar Drugs 2013 857929 PMID: 23762164
Kim et al (2013) Cytotoxic effect of clerosterol isolated from Codium fragile on A2058 human melanoma cells. Mol Cancer Res 11 418 PMID: 23389088
Lee et al (2011) GS28 Protects Neuronal Cell Death Induced by Hydrogen Peroxide under Glutathione-Depleted Condition. Korean J Physiol Pharmacol 15 149 PMID: 21860593
García-Escudero et al (2008) Glioma regression in vitro and in vivo by a suicide combined treatment. Cancer Res 6 407 PMID: 18337448
Greer et al (2018) ONC201 kills breast cancer cells in vitro by targeting mitochondria. Oncotarget 9 18454 PMID: 29719618
Shacham-Silverberg et al (2018) Phosphatidylserine is a marker for axonal debris engulfment but its exposure can be decoupled from degeneration. Cell Death Dis 9 1116 PMID: 30389906
Retamal-Díaz et al (2017) US6 Gene Deletion in Herpes Simplex Virus Type 2 Enhances Dendritic Cell Function and T Cell Activation. Front Immunol 8 1523 PMID: 29176979
Karabay et al (2016) Methylsulfonylmethane Induces p53 Independent Apoptosis in HCT-116 Colon Cancer Cells International Journal of Molecular Sciences 17 1123 PMID: 27428957
Sagher et al (2014) The small molecule NSC676914A is cytotoxic and differentially affects NFκB signaling in ovarian cancer cells and HEK293 cells. Cancer Cell Int 14 75 PMID: 25324692
Nedvetsky et al (2014) Parasympathetic innervation regulates tubulogenesis in the developing salivary gland. Dev Cell 30 449 PMID: 25158854
Nicolson (2014) Recombinant adeno-associated virus utilizes host cell nuclear import machinery to enter the nucleus. J Virol 88 4132 PMID: 24478436
Piao et al (2014) The ethyl acetate fraction of Sargassum muticum attenuates ultraviolet B radiation-induced apoptotic cell death via regulation of MAPK- and caspase-dependent signaling pathways in human HaCaT keratinocytes. Pharm Biol 52 1110 PMID: 24617288
Silva et al (2015) A delay prior to mitotic entry triggers caspase 8-dependent cell death in p53-deficient Hela and HCT-116 cells. Apoptosis 14 1070 PMID: 25602147
Bonnefond et al (2015) Calcium signals inhibition sensitizes ovarian carcinoma cells to anti-Bcl-xL strategies through Mcl-1 down-regulation. Oncoscience 20 535 PMID: 25627260
Amantini et al (2015) Sorafenib induces cathepsin B-mediated apoptosis of bladder cancer cells by regulating the Akt/PTEN pathway. The Akt inhibitor, perifosine, enhances the sorafenib-induced cytotoxicity against bladder cancer cells. Cell Death Dis 2 395 PMID: 26097873
Baron et al (2015) The NLRP3 inflammasome is activated by nanoparticles through ATP, ADP and adenosine. Mol Cell Biol 6 e1629 PMID: 25654762
Hu et al (2015) NF-κB signaling is required for XBP1 (unspliced and spliced)-mediated effects on antiestrogen responsiveness and cell fate decisions in breast cancer. Clin Cancer Res 35 379 PMID: 25368386
Galbiati et al (2014) Role of ROS and HMGB1 in contact allergen-induced IL-18 production in human keratinocytes. J Invest Dermatol 134 2719 PMID: 24780928
Cook et al (2012) Glucose-regulated protein 78 controls cross-talk between apoptosis and autophagy to determine antiestrogen responsiveness. Oncotarget 72 3337 PMID: 22752300
Sullivan et al (2012) ATM and MET kinases are synthetic lethal with nongenotoxic activation of p53. Cell Cycle 8 646 PMID: 22660439
Woo et al (2012) Calpain-mediated processing of p53-associated parkin-like cytoplasmic protein (PARC) affects chemosensitivity of human ovarian cancer cells by promoting p53 subcellular trafficking. J Biol Chem 287 3963 PMID: 22117079
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We pretreated to cultured microglia to inhibit caspases activity at 20microM
AML12 hepatocytes were cultured and were per-treated with Z-VAD-FMK.
12.5 micro molar concentration was used in the study.
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Cell Cycle & DNA Damage Repair Poster
In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.
Huntington's Disease Poster
Huntington's disease (HD) is a monogenic neurodegenerative disorder, which is characterized by the prevalent loss of GABAergic medium spiny neurons (MSN) in the striatum. This poster summarizes the MSN intracellular signaling pathways implicated in the pathology of HD, as well as highlighting the use of iPSCs for HD modeling.
Parkinson's disease (PD) causes chronic disability and is the second most common neurodegenerative condition. This poster outlines the neurobiology of the disease, as well as highlighting current therapeutic treatments for symptomatic PD, and emerging therapeutic strategies to delay PD onset and progression.
Programmed Cell Death Poster
There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.