WS 383

Pricing Availability   Qty
Cat.No. 7055 - WS 383 | C18H20ClN9S2.HCl | CAS No. 2247544-02-9
Description: Potent, selective and reversible DCN1-UBC12 interaction inhibitor
Chemical Name: 5-[[2-[[(4-Chlorophenyl)methyl]thio]-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-yl]thio]-N,N-dimethyl-1H-tetrazole-1-ethanamine hydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (3)

Biological Activity for WS 383

WS 383 is a potent and reversible DCN1-UBC12 interaction inhibitor (IC50 = 11 nM). The compound displays selectivity for DCN1-UBC12 over inhibition of a panel of kinases. including Bruton's tyrosine kinase (BTK), cyclin-dependent kinases (CDKs) and epidermal growth factor receptor (EGFR). In vitro, WS 383 selectively inhibits cullin-3 neddylation over other cullins, and induces accumulation of p21, p27 and NRF2.

Technical Data for WS 383

M. Wt 498.45
Formula C18H20ClN9S2.HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 2247544-02-9
PubChem ID 138319703
InChI Key LJZUAAKUJAXXHQ-UHFFFAOYSA-N
Smiles CN(CCN1N=NN=C1SC2=CC(C)=NC3=NC(SCC4=CC=C(C=C4)Cl)=NN23)C.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for WS 383

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 49.84 100

Preparing Stock Solutions for WS 383

The following data is based on the product molecular weight 498.45. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.01 mL 10.03 mL 20.06 mL
5 mM 0.4 mL 2.01 mL 4.01 mL
10 mM 0.2 mL 1 mL 2.01 mL
50 mM 0.04 mL 0.2 mL 0.4 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References for WS 383

References are publications that support the biological activity of the product.

Wang et al (2019) Development of highly potent, selective, and cellular active triazolo[1,5- a]pyrimidine-based inhibitors targeting the DCN1-UBC12 protein-protein interaction. J.Med.Chem. 62 2772 PMID: 30803229


If you know of a relevant reference for WS 383, please let us know.

View Related Products by Product Action

View all Ubiquitin E3 Ligase Inhibitors

Keywords: WS 383, WS 383 supplier, WS383, ubiquitin, E3, E2, ligase, interaction, inhibitor, inhibits, protein-protein, Cul3/1, neddylation, CRL, cullin-RING, ligases, Ubiquitin, Ligases, Ubiquitin/Ubl, Conjugating, Enzymes, 7055, Tocris Bioscience

Citations for WS 383

Citations are publications that use Tocris products.

Currently there are no citations for WS 383. Do you know of a great paper that uses WS 383 from Tocris? Please let us know.

Reviews for WS 383

There are currently no reviews for this product. Be the first to review WS 383 and earn rewards!

Have you used WS 383?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Targeted Protein Degradation

Targeted Protein Degradation Research Product Guide

This brochure highlights the tools and services available from Bio-Techne to support Targeted Protein Degradation research, including:

  • Active Degraders
  • Degrader Building Blocks
  • Custom Degrader Services
  • UPS Proteins and Assays
  • Assays for Protein Degradation
Programmed Cell Death

Programmed Cell Death Poster

There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.

Targeted Protein Degradation

Targeted Protein Degradation Poster

Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia