W-7 hydrochloride

Pricing Availability   Qty
Description: Calmodulin antagonist. Inhibits myosin light chain kinase
Chemical Name: N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride
Purity: ≥98% (HPLC)
Citations (16)
Reviews (1)

Biological Activity for W-7 hydrochloride

W-7 hydrochloride is a calmodulin antagonist. Inhibits Ca2+-calmodulin-dependent phosphodiesterase (IC50 = 28 μM) and myosin light chain kinase (IC50 = 51 μM).

Compound Libraries for W-7 hydrochloride

W-7 hydrochloride is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for W-7 hydrochloride

M. Wt 377.33
Formula C16H21ClN2O2S.HCl
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 61714-27-0
PubChem ID 124887
Smiles Cl.NCCCCCCNS(=O)(=O)C1=CC=CC2=C(Cl)C=CC=C12

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for W-7 hydrochloride

Solvent Max Conc. mg/mL Max Conc. mM
water 1.89 5 with gentle warming
DMSO 37.73 100

Preparing Stock Solutions for W-7 hydrochloride

The following data is based on the product molecular weight 377.33. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.65 mL 13.25 mL 26.5 mL
5 mM 0.53 mL 2.65 mL 5.3 mL
10 mM 0.27 mL 1.33 mL 2.65 mL
50 mM 0.05 mL 0.27 mL 0.53 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for W-7 hydrochloride

Certificate of Analysis / Product Datasheet
Select another batch:

References for W-7 hydrochloride

References are publications that support the biological activity of the product.

Asano et al (1989) Divergent pharmacological effects of three calmodulin antagonists, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W 7), chlorprom. and calmidazolium, on isometric tension development and myosin light chain phosphorylation in intact bovine trachea J.Pharmacol.Exp.Ther. 251 764 PMID: 2810127

Hidaka et al (1981) N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide, a calmodulin antagonist, inhibits cell proliferation. Proc.Natl.Acad.Sci.U.S.A. 78 4354 PMID: 6945588

Itoh and Hidaka (1984) Direct interaction of calmodulin antagonists with Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase. J.Biochem. 96 1721 PMID: 6099352

Tanaka and Hidaka (1980) Hydrophobic regions function in calmodulin-enzyme(s) interactions. J.Biol.Chem. 255 11078 PMID: 6254958

If you know of a relevant reference for W-7 hydrochloride, please let us know.

View Related Products by Product Action

View all Calcium Binding Protein Antagonists

Keywords: W-7 hydrochloride, W-7 hydrochloride supplier, Calmodulin, antagonists, inhibitors, inhibits, myosin, light, chain, kinases, Ca2+, Binding, Protein, modulators, Calcium, Signaling, Signalling, MLCK, Ca2+-calmodulin-dependent, phosphodiesterase, Myosin, Light, Chain, Kinase, 0369, Tocris Bioscience

16 Citations for W-7 hydrochloride

Citations are publications that use Tocris products. Selected citations for W-7 hydrochloride include:

Mukunda et al (2014) Dimerisation of the Drosophila odorant coreceptor Orco. Neuron 8 261 PMID: 25221476

Yanes and Romero (2009) Dihydrotestosterone stimulates aldosterone secretion by H295R human adrenocortical cells. Mol Cell Endocrinol 303 50 PMID: 19428991

Long et al (2009) Nerve Terminal GABAA Receptors Activate Ca2+/Calmodulin-dependent Signaling to Inhibit Voltage-gated Ca2+ Influx and Glutamate Release. Carcinogenesis 284 8726 PMID: 19141616

Hou et al (2015) The Actin Nucleator Cobl Is Controlled by Calcium and Calmodulin. Apoptosis 13 e1002233 PMID: 26334624

Bonnefond et al (2015) Calcium signals inhibition sensitizes ovarian carcinoma cells to anti-Bcl-xL strategies through Mcl-1 down-regulation. J Biol Chem 20 535 PMID: 25627260

Araki et al (2015) Rapid dispersion of SynGAP from synaptic spines triggers AMPA receptor insertion and spine enlargement during LTP. J Neurosci 85 173 PMID: 25569349

Schiff et al (2019) P38 inhibition reverses TGFβ1 and TNFα-induced contraction in a model of proliferative vitreoretinopathy. Commun Biol 2 162 PMID: 31069271

Gray and Golowasch (2016) Voltage Dependence of a Neuromodulator-Activated Ionic Current. Eneuro 3 PMID: 27257619

Abdou et al (2013) The calcium signaling pathway regulates leydig cell steroidogenesis through a transcriptional cascade involving the nuclear receptor NR4A1 and the steroidogenic acute regulatory protein. Endocrinology 154 511 PMID: 23183170

Haag et al (2018) The Actin Nucleator Cobl Is Critical for Centriolar Positioning, Postnatal Planar Cell Polarity Refinement, and Function of the Cochlea. Cell Rep 24 2418 PMID: 30157434

Lopez et al (2022) Ketamine exerts its sustained antidepressant effects via cell-type-specific regulation of Kcnq2. Neuron 110 2283 PMID: 35649415

Dai et al (2011) Phospholipase C, Ca2+, and calmodulin signaling are required for 5-HT2A receptor-mediated transamidation of Rac1 by transglutaminase. Front Cell Neurosci 213 403 PMID: 20717650

Yanda et al (2017) Histone deacetylase 6 inhibition reduces cysts by decreasing cAMP and Ca(2+) in knock-out mouse models of polycystic kidney disease. J Biol Chem 292 17897 PMID: 28887310

Yuan et al (2015) Protein kinase G-regulated production of H2S governs oxygen sensing. Sci Signal 8 ra37 PMID: 25900831

Chang et al (2013) Areca nut-induced buccal mucosa fibroblast contraction and its signaling: a potential role in oral submucous fibrosis--a precancer condition. Psychopharmacology (Berl) 34 1096 PMID: 23349021

Midkiff et al (2011) Small molecule inhibitors of the Candida albicans budded-to-hyphal transition act through multiple signaling pathways. PLoS One 6 e25395 PMID: 21966518

Do you know of a great paper that uses W-7 hydrochloride from Tocris? Please let us know.

Reviews for W-7 hydrochloride

Average Rating: 5 (Based on 1 Review.)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used W-7 hydrochloride?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

PN and PH cells were treated with W-7 (catalog no. 0369) and W-13 (catalog no. 0361) purchased from Tocris..
By Anonymous on 11/17/2019
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: PN and PH cells

PN and PH cells were treated with W-7 (catalog no. 0369) and W-13(catalog no. 0361) purchased from Tocris.

PMID: 28887310
review image