Topotecan hydrochloride

Pricing Availability   Qty
Description: DNA topoisomerase I inhibitor; Camptothecin (Cat. No. 1100) analog
Alternative Names: SK&F 104864-A
Chemical Name: 10-[(Dimethylamino)methyl]-4-ethyl-4,9-dihydroxy-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione hydrochloride
Purity: ≥98% (HPLC)
Citations (6)
Literature (3)

Biological Activity for Topotecan hydrochloride

Topotecan hydrochloride is a DNA topoisomerase I inhibitor; induces rapid apoptotic death in human B-lineage acute lymphoblastic leukemia (ALL) cells. Improves survival in three mouse models of poor prognosis ALL; downregulates B-Myb and MycN expression in neuroblastoma cells. Water-soluble analog of Camptothecin (Cat. No. 1100). Orally bioavailable. Topotecan disrupts the interaction between NHP2L1 and U4 RNA by binding to U4 and inhibits RNA splicing.

Technical Data for Topotecan hydrochloride

M. Wt 457.91
Formula C23H23N3O5.HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 119413-54-6
PubChem ID 60699
Smiles OC1=CC=C(N=C(C(N3C4)=CC([C@](CC)(O)C(OC5)=O)=C5C3=O)C4=C2)C2=C1CN(C)C.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Topotecan hydrochloride

Solvent Max Conc. mg/mL Max Conc. mM
water 45.79 100
DMSO 45.79 100

Preparing Stock Solutions for Topotecan hydrochloride

The following data is based on the product molecular weight 457.91. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.18 mL 10.92 mL 21.84 mL
5 mM 0.44 mL 2.18 mL 4.37 mL
10 mM 0.22 mL 1.09 mL 2.18 mL
50 mM 0.04 mL 0.22 mL 0.44 mL

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Product Datasheets for Topotecan hydrochloride

Certificate of Analysis / Product Datasheet
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References for Topotecan hydrochloride

References are publications that support the biological activity of the product.

Uckun et al (1995) In vitro and in vivo activity of topo. against human B-lineage acute lymphoblastic leukemia cells. Blood 85 2817 PMID: 7742543

Sottile et al (2012) A chemical screen identifies the chemotherapeutic drug topo. as a specific inhibitor of the B-MYB/MYCN axis in neuroblastoma. Oncotarget. 3 535 PMID: 22619121

Schellens et al (1996) Bioavailability and pharmacokinetics of oral topotecan: a new topoisomerase I inhibitor. Br.J.Cancer 73 1268 PMID: 8630291

If you know of a relevant reference for Topotecan hydrochloride, please let us know.

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View all DNA Topoisomerase Inhibitors

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6 Citations for Topotecan hydrochloride

Citations are publications that use Tocris products. Selected citations for Topotecan hydrochloride include:

Jen Q et al (2022) Ion Channel Drugs Suppress Cancer Phenotype in NG108-15 and U87 Cells: Toward Novel Electroceuticals for Glioblastoma. Cancers (Basel) 14 PMID: 35326650

Martin et al (2017) Molecular Phenotyping Combines Molecular Information, Biological Relevance, and Patient Data to Improve Productivity of Early Drug Discovery. Cell Chem Biol 24 624-634.e3 PMID: 28434878

Florea et al (2017) Calcium-regulatory proteins as modulators of chemotherapy in human neuroblastoma. Oncotarget 8 22876 PMID: 28206967

Pearson et al (2016) Identification of chemicals that mimic transcriptional changes associated with autism, brain aging and neurodegeneration. Int J Mol Med 7 11173 PMID: 27029645

Do you know of a great paper that uses Topotecan hydrochloride from Tocris? Please let us know.

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Literature in this Area

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