Pricing Availability   Qty
Description: Potent inhibitor of SERCA ATPase
Chemical Name: (3S,3aR,4S,6S,6AR,7S,8S,9bS)-6-(Acetyloxy)-2,3,3a,4,5,6,6a,7,8,9b-decahydro-3,3a-dihydroxy-3,6,9-trimethyl-8-[[(2Z)-2-methyl-1-oxo-2-butenyl]oxy]-2-oxo-4-(1-oxobutoxy)azuleno[4,5-b]furan-7-yl octanoate
Purity: ≥97% (HPLC)
Citations (42)
Reviews (7)

Biological Activity for Thapsigargin

Thapsigargin is a potent inhibitor of sarco/endoplasmic reticulum Ca2+-ATPases (SERCA) that causes endoplasmic reticulum (ER) stress. Thapsigargin can be used to induce autophagy in mammalian cells. In vitro, thapsigargin blocks infection of immortilized and primary human cells with respiratory syncytial virus (RSV), SARS-CoV-2 and influenza A. Thapsigargin protects mice against lethal influenza A viral infection and reduces virus titres in the lungs of treated mice.

Technical Data for Thapsigargin

M. Wt 650.76
Formula C34H50O12
Storage Store at -20°C
Purity ≥97% (HPLC)
CAS Number 67526-95-8
PubChem ID 446378
Smiles O=C(O[C@@H]2[C@@]3(O)[C@@H](OC([C@](C)3O)=O)C1=C(C)[C@H](OC(/C(C)=C\C)=O)[C@@H](OC(CCCCCCC)=O)[C@@]([H])1[C@](OC(C)=O)(C)C2)CCC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Thapsigargin

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 65.08 100

Preparing Stock Solutions for Thapsigargin

The following data is based on the product molecular weight 650.76. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.54 mL 7.68 mL 15.37 mL
5 mM 0.31 mL 1.54 mL 3.07 mL
10 mM 0.15 mL 0.77 mL 1.54 mL
50 mM 0.03 mL 0.15 mL 0.31 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Product Datasheets for Thapsigargin

Certificate of Analysis / Product Datasheet
Select another batch:

View Related Products by Product Action

View all Ca2+-ATPase Inhibitors

Keywords: Thapsigargin, Thapsigargin supplier, Potent, inhibitors, inhibits, SERCA, ATPase, Ca2+, modulators, Ca2+-ATPase, Signaling, Signalling, Calcium-ATPase, P-type, Transporters, Ion, Pumps, Autophagy, Coronavirus, Influenza, Viruses, 1138, Tocris Bioscience

42 Citations for Thapsigargin

Citations are publications that use Tocris products. Selected citations for Thapsigargin include:

Florea et al (2017) Calcium-regulatory proteins as modulators of chemotherapy in human neuroblastoma. Oncotarget 8 22876 PMID: 28206967

Deldicque et al (2011) ER stress induces anabolic resistance in muscle cells through PKB-induced blockade of mTORC1. PLoS One 6 e20993 PMID: 21698202

Meitzen et al (2011) β1-Adrenergic receptors activate two distinct signaling pathways in striatal neurons. J Neurochem 116 984 PMID: 21143600

Li and Burrell (2011) Associative, bidirectional changes in neural signaling utilizing NMDA receptor- and endocannabinoid-dependent mechanisms. Learn Mem 18 545 PMID: 21844187

McEwan et al (2021) Autocrine regulation of wound healing by ATP release and P2Y 2 receptor activation Life Sci 283 119850 PMID: 34314735

Glanowska and Moenter (2015) Differential regulation of GnRH secretion in the preoptic area (POA) and the median eminence (ME) in male mice. Neuropharmacology 156 231 PMID: 25314270

Podszywalow-Bartnicka et al (2014) Downregulation of BRCA1 protein in BCR-ABL1 leukemia cells depends on stress-triggered TIAR-mediated suppression of translation. Biochim Biophys Acta 13 3727 PMID: 25483082

Kondratska et al (2014) Orai1 and STIM1 mediate SOCE and contribute to apoptotic resistance of pancreatic adenocarcinoma. PLoS One 1843 2263 PMID: 24583265

Soltoff and Lannon (2013) Activation of ERK1/2 by store-operated calcium entry in rat parotid acinar cells. PLoS One 8 e72881 PMID: 24009711

Grassin-Delyle et al (2013) The expression and relaxant effect of bitter taste receptors in human bronchi. Respir Res 14 134 PMID: 24266887

Semenov et al (2013) Recruitment of the intracellular Ca2+ by ultrashort electric stimuli: the impact of pulse duration. Cell Calcium 54 145 PMID: 23777980

Varadarajan et al (2013) Endoplasmic reticulum membrane reorganization is regulated by ionic homeostasis. PLoS One 8 e56603 PMID: 23457590

Pinheiro et al (2013) Bradykinin-induced Ca2+ signaling in human subcutaneous fibroblasts involves ATP release via hemichannels leading to P2Y12 receptors activation. Cell Commun Signal 11 70 PMID: 24047499

Moore et al (2009) Plasticity of burst firing induced by synergistic activation of metabotropic glutamate and acetylcholine receptors. Neuron 61 287 PMID: 19186170

Szikra et al (2009) Calcium homeostasis and cone signaling are regulated by interactions between calcium stores and plasma membrane ion channels. PLoS One 4 e6723 PMID: 19696927

Gerich et al (2009) H(2)O(2)-mediated modulation of cytosolic signaling and organelle function in rat hippocampus. J Neurosci 458 937 PMID: 19430810

Shemorry et al (2019) Caspase-mediated cleavage of IRE1 controls apoptotic cell commitment during endoplasmic reticulum stress. Elife 8 e47084 PMID: 31453810

Andrade-Talavera et al (2016) Presynaptic Spike Timing-Dependent Long-Term Depression in the Mouse Hippocampus. Cereb Cortex 26 3637 PMID: 27282393

Salzer et al (2016) Control of sensory neuron excitability by serotonin involves 5HT2C receptors and Ca2+-activated chloride channels. Neuropharmacology 110 (A) 277 PMID: 27511837

Azab et al (2015) Binding of αherpesvirus Glycoprotein H to Surface α4β1-Integrins Activates Calcium-Signaling Pathways and Induces Phosphatidylserine Exposure on the Plasma Membrane. Endocrinology 6 e01552-15 PMID: 26489864

Velásquez-Martínez et al (2015) α-1 adrenoreceptors modulate GABA release onto ventral tegmental area DA neurons. Pflugers Arch 88 110 PMID: 25261018

Dobson et al (2015) Caffeine Modulates Vesicle Release and Recovery at Cerebellar Parallel Fibre Terminals, Independently of Calcium and Cyclic AMP Signalling. J Neuroinflammation 10 e0125974 PMID: 25933382

Shambharkar et al (2015) TMEM203 Is a Novel Regulator of Intracellular Calcium Homeostasis and Is Required for Spermatogenesis. J Cell Biol 10 e0127480 PMID: 25996873

Cao et al (2015) Calcium release through P2X4 activates calmodulin to promote endolysosomal membrane fusion. PLoS One 209 879 PMID: 26101220

Egawa et al (2013) Optogenetic probing and manipulation of the calyx-type presynaptic terminal in the embryonic chick ciliary ganglion. PLoS One 8 e59179 PMID: 23555628

Rojas et al (2013) Activation of group I metabotropic glutamate receptors potentiates heteromeric kainate receptors. Mol Pharmacol 83 106 PMID: 23066089

Few et al (2012) Asynchronous Ca2+ current conducted by voltage-gated Ca2+ (CaV)-2.1 and CaV2.2 channels and its implications for asynchronous neurotransmitter release. Proc Natl Acad Sci U S A 109 E452 PMID: 22308469

Xu et al (2018) Increased sensitivity to apoptosis upon endoplasmic reticulum stress-induced activation of the unfolded protein response in chemotherapy-resistant malignant pleural mesothelioma. Br J Cancer 119 65 PMID: 29921948

Gupta et al (2016) NCOA3 coactivator is a transcriptional target of XBP1 and regulates PERK-eIF2α-ATF4 signalling in breast cancer. Oncogene 35 5860 PMID: 27109102

Earls et al (2010) Dysregulation of presynaptic calcium and synaptic plasticity in a mouse model of 22q11 deletion syndrome. PLoS One 30 15843 PMID: 21106823

He et al (2018) Mechanical regulation of stem-cell differentiation by the stretch-activated Piezo channel. Nature 555 103 PMID: 29414942

Abuaita et al (2018) Mitochondria-Derived Vesicles Deliver Antimicrobial Reactive Oxygen Species to Control Phagosome-Localized Staphylococcus aureus. Cell Host Microbe 24 625 PMID: 30449314

Atakpa et al (2018) IP3 Receptors Preferentially Associate with ER-Lysosome Contact Sites and Selectively Deliver Ca2+ to Lysosomes. Cell Rep 25 3180 PMID: 30540949

Lambelet et al (2018) Dysfunctional autophagy following exposure to pro-inflammatory cytokines contributes to pancreatic β-cell apoptosis. Cell Death Dis 9 96 PMID: 29367588

Hossain et al (2018) Hyperactivation of nuclear receptor coactivators induces PERK-dependent cell death. Oncotarget 9 11707 PMID: 29545931

Mätlik et al (2016) Role of two sequence motifs of mesencephalic astrocyte-derived neurotrophic factor in its survival-promoting activity. Cell Death Dis 6 e2032 PMID: 26720341

Kelm et al (2008) The role of protein kinase A in the ethanol-induced increase in spontaneous GABA release onto cerebellar Purkinje neurons. J Neurophysiol 100 3417 PMID: 18945815

Dai et al (2008) A crucial role for cAMP and protein kinase A in D1 DA receptor regulated intracellular calcium transients. Neurosignals 16 112 PMID: 18253052

Haeberle et al Swelling-activated Ca2+ channels trigger Ca2+ signals in Merkel cells. MBio 3 e1750 PMID: 18454189

Bender et al (2006) Two coincidence detectors for spike timing-dependent plasticity in somatosensory cortex. J Neurosci 26 4166 PMID: 16624937

Fransen et al (2006) Mechanism of graded persistent cellular activity of entorhinal cortex layer v neurons. Neuron 49 735 PMID: 16504948

Kelm et al (2007) Calcium release from presynaptic internal stores is required for ethanol to increase spontaneous γ-aminobutyric acid release onto cerebellum Purkinje neurons. Cell Cycle 323 356 PMID: 17652632

Do you know of a great paper that uses Thapsigargin from Tocris? Please let us know.

Reviews for Thapsigargin

Average Rating: 4.4 (Based on 7 Reviews.)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Thapsigargin?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Good activator for SOCE.
By Yuyang Sun on 05/30/2022
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Macrophage

This product significantly induced calcium release and store operated calcium entry.

review image

Usage Information.
By Anonymous on 06/20/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: KMS11 or OPM2

100 nM

Dissolved in DMSO. We treated cells for 16 hr.

PMID: 31453810 Reference
review image

Thapsigargin Blocks Re-Uptake of Ca2+ Into ER leading to increased T-cell activation by IFNg.
By Li Zhu on 01/23/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: Primary Cd8+ T-cell

Thapsigargin was used to block SERCA in activated CD8+ T-cells. This maintained high intracellular Ca2+, leading to increased activation of CD8+ T-cells as measured by IFNg production via intracellular flow cytometry.

review image

Intracellular calcium depletion.
By Anonymous on 11/19/2018
Assay Type: Ex Vivo
Species: Mouse

This compound is often used to deplete intracellular calcium stores in cell culture or other applications. It worked as expected in our tissue preparations.

Thapsigargin elicits store-operated Ca2+ entry after ER store depletion in RBL-2H3 cells..
By Niroj Shrestha on 10/27/2018
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: RBL-2H3

Cytosolic Ca2+ sensitive indicator, Fura-2 was loaded in RBL-2H3 cells and excited alternately at 340 and 380 nm and fluorescence was captured at 510 nm.ER store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry via STIM1/Orai1 pathway into the cells upon addition of 2 mM extracellular Ca2+.RBL-2H3 cells were loaded with FURA-2AM (2 μM) and Pluronic F127 (0.02%) for 30 min followed by washing with normal-Tyrode solution. Cells were stimulated at 1 Hz frequency and fluorescence intensities were measured.

review image

XBP1 splicing.
By Preethi Vijayaraj on 05/01/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: mesenchymal cells

Used it as a positive control to test if the mechanism of a novel small molecule (compound x) was to reduce ER stress. Cells were treated with 5um Thapsigargin for 24 hours, and splicing of XBP1 was determined by semi-quantitative RT-PCR

review image

Thapsigargin calcium assay.
By Anonymous on 12/12/2017
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK 293T

for calcium release from the ER