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Submit ReviewTES, free acid
Description: Buffering agent; pKa at physiolgical pH (7.4) at 25°C
Chemical Name: N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid
Purity: ≥99%
Biological Activity
Buffering agent. Working pH range: 6.8 - 8.2. used in a range of protein assays. Forms complexes with DNA and affects restriction enzyme kinetics.
Technical Data
| M. Wt | 229.25 |
| Formula | C6H15NO6S |
| Storage | Store at RT |
| Purity | ≥99% |
| CAS Number | 7365-44-8 |
| PubChem ID | 81831 |
| InChI Key | JOCBASBOOFNAJA-UHFFFAOYSA-N |
| Smiles | OCC(NCCS(=O)(O)=O)(CO)CO |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 22.93 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 229.25. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.36 mL | 21.81 mL | 43.62 mL |
| 5 mM | 0.87 mL | 4.36 mL | 8.72 mL |
| 10 mM | 0.44 mL | 2.18 mL | 4.36 mL |
| 50 mM | 0.09 mL | 0.44 mL | 0.87 mL |
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Product Datasheets
References
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Reviews for TES, free acid
Average Rating: 5 (Based on 1 Review.)
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TES free acid makes good buffer.
By
Anonymous on 02/11/2019
Species: Human
TES dissolved in dH2O to prepare buffer. 10N NaOH used to adjust pH.I will recommand buying the product.
Store at 4ᵒC.