Temozolomide

Pricing Availability Delivery Time Qty
Cat.No. 2706 - Temozolomide | C6H6N6O2 | CAS No. 85622-93-1
Description: DNA-methylating antitumor agent
Alternative Names: NSC 362856, CCRG 81045
Chemical Name: 3,4-Dihydro-3-methyl-4-oxoimidazo[5,1-d]-1,2,3,5-tetrazine-8-carboxamide
Purity: ≥99% (HPLC)
Datasheet
Citations (4)
Reviews
Literature
Pathways

Biological Activity

DNA methylating, chemotherapeutic agent. Displays antitumor activity against a board spectrum of tumors, including leukemias, lymphomas and solid tumors (IC50 = 5.0 μM for cytotoxicity against mouse TLX5 lymphoma cells). Induces autophagy in malignant glioma cells. Exhibits enhanced activity when used in combination with CHC (Cat No. 5029).

Compound Libraries

Temozolomide is also offered as part of the Tocriscreen Plus, Tocriscreen Epigenetics Toolbox and Tocriscreen Library of FDA-Approved Compounds. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 194.15
Formula C6H6N6O2
Storage Store at RT
Purity ≥99% (HPLC)
CAS Number 85622-93-1
PubChem ID 5394
InChI Key BPEGJWRSRHCHSN-UHFFFAOYSA-N
Smiles CN1N=NC2=C(N=CN2C1=O)C(N)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 9.71 50

Preparing Stock Solutions

The following data is based on the product molecular weight 194.15. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.15 mL 25.75 mL 51.51 mL
5 mM 1.03 mL 5.15 mL 10.3 mL
10 mM 0.52 mL 2.58 mL 5.15 mL
50 mM 0.1 mL 0.52 mL 1.03 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Plowman et al (1994) Preclinical antitumor activity of temozolomide in mice: efficacy against human brain tumor xenografts and synergism with 1,3-bis(2-chloroethyl)-1-nitrosourea. Cancer Res. 54 3793 PMID: 8033099

Clark et al (1995) Antitumor imidazotetraazines. 32. Synthesis of novel imidazotetrazinones and related bicyclic heterocycles to probe the mode of action of the antitumor drug temozolomide. J.Med.Chem. 38 1493 PMID: 7739008

Bodell et al (2003) Formation of DNA adducts and induction of lacI mutations in big blue rat-2 cells treated with temozolomide: implications for the treatment of low-grade adult and pediatric tumors. Cancer Epidemiol.Biomarkers Prevent. 12 545 PMID:

Kanzawa et al (2004) Role of autophagy in temozolomide-induced cytotoxicity for malignant glioma cells. Cell Death Differ. 11 448 PMID: 14713959


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Keywords: Temozolomide, supplier, DNA-methylating, antitumor, agent, Apoptosis, Inducer, NSC362856, CCRG81045, chemotherapeutics, NSC, 362856, CCRG, 81045, Apoptosis, Inducers, Autophagy, Apoptosis, Inducers, Tocris Bioscience

4 Citations for Temozolomide

Citations are publications that use Tocris products. Selected citations for Temozolomide include:

Pastori et al (2014) BET bromodomain proteins are required for glioblastoma cell proliferation. Epigenetics 9 611 PMID: 24496381

Canello et al (2014) Antineoplastic effect of decoy oligonucleotide derived from MGMT enhancer. PLoS One 9 e113854 PMID: 25460932

Johannessen et al (2013) The DNA repair protein ALKBH2 mediates temozolomide resistance in human glioblastoma cells. Neuro Oncol 15 269 PMID: 23258843

Hu et al (2012) Fibulin-3 promotes glioma growth and resistance through a novel paracrine regulation of Notch signaling. Cancer Res 72 3873 PMID: 22665268


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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

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Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Programmed Cell Death

Programmed Cell Death Poster

There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.

Pathways for Temozolomide

Apoptosis

Apoptosis Signaling Pathway

Apoptosis is a physiological process for cell death that is critical during aging and development. It may also be referred to as cell 'suicide'. Apoptosis can be triggered by events both inside and outside of the cell.