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Description: Fluorogenic ligand for Broccoli RNA aptamer
Chemical Name: (Z)-3-((1H-Benzo[d]imidazol-4-yl)methyl)-5-(3,5-difluoro-4-hydroxybenzylidene)-2-thioxoimidazolidin-4-one
Purity: ≥98% (HPLC)
Literature (1)

Biological Activity for TBI

TBI is a DFHBI derivative for imaging of single Broccoli-tagged mRNA in cells. Excitation and emission maxima (λ) when bound to Broccoli are 485 and 527 nm, respectively. TBI shows higher affinity for Broccoli aptamers compared with DFHBI-1T (Cat. No. 5610) (KD values are 71 and 305 nM, respectively). Extinction coefficient = 35,100 M-1 cm-1.

Licensing Information

Sold under license from Lucerna

Optical Data for TBI

Emission Color Green
λabs 485 nm
λem 527 nm
Extinction Coefficient (ε) 35100 M-1cm-1
Quantum Yield (φ) 0.64
Application Live-cell Imaging, Fluorescence Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for TBI

M. Wt 386.38
Formula C18H12F2N4O2S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 2863639-29-4
Smiles FC1=C(C(F)=CC(/C=C2NC(N(C\2=O)CC3=C4N=CNC4=CC=C3)=S)=C1)O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for TBI

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 7.73 20

Preparing Stock Solutions for TBI

The following data is based on the product molecular weight 386.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 12.94 mL 64.7 mL 129.41 mL
1 mM 2.59 mL 12.94 mL 25.88 mL
2 mM 1.29 mL 6.47 mL 12.94 mL
10 mM 0.26 mL 1.29 mL 2.59 mL

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References for TBI

References are publications that support the biological activity of the product.

Li et al (2021) Engineering fluorophore recycling in a fluorogenic RNA aptamer. Angew.Chem.Int.Ed.Engl. 60 24153 PMID: 34490956

If you know of a relevant reference for TBI, please let us know.

View Related Products by Target

Keywords: TBI, TBI supplier, fluorescence, fluorophores, fluorophore, single, RNA, imaging, fluorescent, protein, Broccoli, aptamers, Aptamer-based, Imaging, 7660, Tocris Bioscience

Citations for TBI

Citations are publications that use Tocris products.

Currently there are no citations for TBI. Do you know of a great paper that uses TBI from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals