ShK-Dap22

Pricing Availability Delivery Time Qty
Cat.No. 3220 - ShK-Dap22 | Arg-Ser-Cys-Ile-Asp-Thr-Ile-Pro-Lys-Ser-Arg-Cys-Thr-Ala-Phe-Gln-Cys-Lys-His-Ser-Met-Dap-Tyr-Arg-Leu-Ser-Phe-Cys-Arg-Lys-Thr-Cys-Gly-Thr-Cys | CAS No. 220384-25-8
Description: Extremely potent and selective KV1.3 blocker; suppresses T cell activation in vitro
Datasheet
Citations
Literature

Biological Activity

Extremely potent KV1.3 channel blocker (Kd = 23 pM for mKV1.3 currents). Selective for KV1.3 over other mammalian potassium channels (IC50 values are 23, 1800, 10500, 37000 and 39000 pM for mKV1.3, mKV1.1, hKV1.6, mKV1.4 and rKV1.2 respectively, and >100000 pM for hKV1.5, mKV1.7, hKV3.1, rKV3.4 and hKCa4). Suppresses T cell activation in vitro (IC50 < 500 pM).

Technical Data

M. Wt 4012.7
Formula C166H268N54O48S7
Sequence RSCIDTIPKSRCTAFQCKHSMXYRLSFCRKTCGTC

(Modifications: Disulfide bridges: 3-35,12-28,17-32), X= Dap)

Storage Store at -20°C
CAS Number 220384-25-8
PubChem ID 122187345
InChI Key PFWOJTLVNOZSGQ-RIMJLNMDSA-N
Smiles [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@H]1CSSC[C@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H]2CSSC[C@@H]3NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC4=CC=CC=C4)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CSSC[C@H](NC(=O)[C@H](CC4=CC=CC=C4)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC4=CC=C(O)C=C4)NC(=O)[C@H](CN)NC(=O)[C@H](CCSC)NC(=O)[C@H](CO)NC(=O)[C@H](CC4=CNC=N4)NC(=O)[C@H](CCCCN)NC3=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(=O)N2)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@@H]2CCCN2C(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC1=O)[C@@H](C)CC)[C@@H](C)O)[C@@H](C)CC)[C@@H](C)O)[C@@H](C)O)C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

SolubilitySoluble to 1 mg/ml in water

Preparing Stock Solutions

The following data is based on the product molecular weight 4012.7. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 0.25 mL 1.25 mL 2.49 mL
5 mM 0.05 mL 0.25 mL 0.5 mL
10 mM 0.02 mL 0.12 mL 0.25 mL
50 mM 0 mL 0.02 mL 0.05 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Kalman et al (1998) ShK-Dap22, a potent KV1.3-specific immunosuppresive polypeptide. J.Biol.Chem. 273 32697 PMID: 9830012

Robbins et al (2005) Hypoxia modulates early events in T cell receptor-mediated activation in human T lymphocytes via KV1.3 channels. J.Physiol. 564 131 PMID: 15677684


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Citations for ShK-Dap22

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Literature in this Area

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