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Fluorescently labelled peptide derived from human ACE2 (SBP1, Cat. No. 7233). Composed of SBP1 conjugated to FITC (Fluorescein-5-isothiocyanate; Cat. No. 5440). SBP1 binds receptor binding domain (RBD) of insect-derived SARS-CoV-2 spike protein (KD = 1.3 μM for N-terminal biotinylated, insect-derived spike protein RBD; see Zhang et al preprint publication).
Please note - unpublished, in-house data suggests SBP1 has approximately 75-fold lower affinity for insect-derived SARS-CoV-2 RBD than reported by Zhang et al.
(Modifications: Ile-1 = FITC-PEG4-Ile, Ser-23 = C-terminal amide)
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solubility||Soluble to 1 mg/ml in 0.1M PBS (pH 7.4)|
References are publications that support the biological activity of the product.
Zhang et al (2020) The first-in-class peptide binder to the SARS-CoV-2 spike protein. BioRxiv - Paper not yet peer reviewed.
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Keywords: SBP1-FITC SBP1-FITC, SBP1-FITC SBP1-FITC supplier, spike, binding, protein, SARS-CoV-2, receptor, domain, RBD, COVID-19, severe, acute, respiratory, syndrome, ACE2, peptide, fluorescently-labeled, flluorescence, Enzyme, Probes, and, Substrates, 7232, Tocris Bioscience
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