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Description: Green fluorescent dye
Alternative Names: Fluorescein-5-Isothiocyanate,FITC Isomer 1
Chemical Name: 3',6'-Dihydroxy-5-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
Purity: ≥95% (HPLC)
Citations (1)
Literature (1)

Biological Activity for FITC

Key information: FITC (an amine-reactive version of the parent dye, fluorescein) is a widely-used green fluorescent dye supplied with an isothiocyanate reactive group for the labeling of primary amines. FITC is bright and has good water-solubility, however it is sensitive to changes in pH and temperature, and has a relatively high photobleaching rate.

Application: Suitable for flow cytometry, immunofluorescence (IHC, ICC), fluorescence microscopy, fluorescence in situ hybridization (FISH), biochemical assays.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 495 nm and 525 nm, respectively; quantum yield = 0.92; extinction coefficient = 75,000 M-1cm-1; A280 = 0.35.

Optical Data for FITC

FITC Dye Spectra
Emission Color Green
λabs 495 nm
λem 525 nm
Extinction Coefficient (ε) 75000 M-1cm-1
Quantum Yield (φ) 0.92
Closest Laser line 488 nm
Reactive Group Isothiocyanate
Reactivity Primary amines
Cell Permeable Yes
Correction Factor 280 0.35
Application Flow Cytometry, Immunofluorescence (IHC, ICC), Fluorescence Microscopy, FISH

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for FITC

M. Wt 389.38
Formula C21H11NO5S
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 3326-32-7
PubChem ID 18730
Smiles OC1=CC=C(C2(C(C=CC(N=C=S)=C3)=C3C(O2)=O)C(C=CC(O)=C4)=C4O5)C5=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for FITC

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 38.94 100

Preparing Stock Solutions for FITC

The following data is based on the product molecular weight 389.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.57 mL 12.84 mL 25.68 mL
5 mM 0.51 mL 2.57 mL 5.14 mL
10 mM 0.26 mL 1.28 mL 2.57 mL
50 mM 0.05 mL 0.26 mL 0.51 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

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Dilution Calculator

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Product Datasheets for FITC

Certificate of Analysis / Product Datasheet
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References for FITC

References are publications that support the biological activity of the product.

Yang et al (2014) A novel CXCR4-selective high-affinity fluorescent probe and its application in competitive binding assays. Biochemistry 53 4881 PMID: 25058910

If you know of a relevant reference for FITC, please let us know.

Keywords: FITC, FITC supplier, fluorescent, reagents, labeling, amines, Fluorescein-5-Isothiocyanate, Isomer1, dyes, FITC, Isomer, 1, Standard, Fluorescein,, Coumarin, and, Rhodamine, Dyes, Flow, Cytometry, 5440, Tocris Bioscience

1 Citation for FITC

Citations are publications that use Tocris products. Selected citations for FITC include:

Meyer et al (2012) Lipidic nanocapsule drug delivery: neuronal protection for cochlear implant optimization. Int J Nanomedicine 7 2449 PMID: 22654517

Do you know of a great paper that uses FITC from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals