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Submit ReviewFITC
Description: Green fluorescent dye
Alternative Names: Fluorescein-5-Isothiocyanate,FITC Isomer 1
Chemical Name: 3',6'-Dihydroxy-5-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
Purity: ≥95% (HPLC)
Biological Activity for FITC
Key information: FITC (an amine-reactive version of the parent dye, fluorescein) is a widely-used green fluorescent dye supplied with an isothiocyanate reactive group for the labeling of primary amines. FITC is bright and has good water-solubility, however it is sensitive to changes in pH and temperature, and has a relatively high photobleaching rate.
Application: Suitable for flow cytometry, immunofluorescence (IHC, ICC), fluorescence microscopy, fluorescence in situ hybridization (FISH), biochemical assays.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 495 nm and 525 nm, respectively; quantum yield = 0.92; extinction coefficient = 75,000 M-1cm-1; A280 = 0.35.
Optical Data for FITC
| Emission Color | Green |
|---|---|
| Brightness | |
| λabs | 495 nm |
| λem | 525 nm |
| Extinction Coefficient (ε) | 75000 M-1cm-1 |
| Quantum Yield (φ) | 0.92 |
| Closest Laser line | 488 nm |
| Reactive Group | Isothiocyanate |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.35 |
| Application | Flow Cytometry, Immunofluorescence (IHC, ICC), Fluorescence Microscopy, FISH |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for FITC
| M. Wt | 389.38 |
| Formula | C21H11NO5S |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 3326-32-7 |
| PubChem ID | 18730 |
| InChI Key | MHMNJMPURVTYEJ-UHFFFAOYSA-N |
| Smiles | OC1=CC=C(C2(C(C=CC(N=C=S)=C3)=C3C(O2)=O)C(C=CC(O)=C4)=C4O5)C5=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for FITC
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 38.94 | 100 |
Preparing Stock Solutions for FITC
The following data is based on the product molecular weight 389.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.57 mL | 12.84 mL | 25.68 mL |
| 5 mM | 0.51 mL | 2.57 mL | 5.14 mL |
| 10 mM | 0.26 mL | 1.28 mL | 2.57 mL |
| 50 mM | 0.05 mL | 0.26 mL | 0.51 mL |
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Product Datasheets for FITC
References for FITC
References are publications that support the biological activity of the product.
Yang et al (2014) A novel CXCR4-selective high-affinity fluorescent probe and its application in competitive binding assays. Biochemistry 53 4881 PMID: 25058910
If you know of a relevant reference for FITC, please let us know.
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Keywords: FITC, FITC supplier, fluorescent, reagents, labeling, amines, Fluorescein-5-Isothiocyanate, Isomer1, dyes, FITC, Isomer, 1, Standard, Fluorescein,, Coumarin, and, Rhodamine, Dyes, Flow, Cytometry, 5440, Tocris Bioscience
1 Citation for FITC
Citations are publications that use Tocris products. Selected citations for FITC include:
Meyer et al (2012) Lipidic nanocapsule drug delivery: neuronal protection for cochlear implant optimization. Int J Nanomedicine 7 2449 PMID: 22654517
Do you know of a great paper that uses FITC from Tocris? Please let us know.
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Literature in this Area
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Fluorescent Dyes and Probes Research Product GuideUpdated
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including Janelia Fluor® Dyes
- Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
- Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
- Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals
- Tissue Clearing Kits and Reagents