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Submit ReviewFluorescent reagent for the labeling of amines. Excitation maximum ~ 495 nm; emission maximum ~ 525 nm.
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral ViewerM. Wt | 389.38 |
Formula | C21H11NO5S |
Storage | Store at -20°C |
Purity | ≥98% (HPLC) |
CAS Number | 3326-32-7 |
PubChem ID | 18730 |
InChI Key | MHMNJMPURVTYEJ-UHFFFAOYSA-N |
Smiles | OC1=CC=C(C2(C(C=CC(N=C=S)=C3)=C3C(O2)=O)C(C=CC(O)=C4)=C4O5)C5=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solvent | Max Conc. mg/mL | Max Conc. mM | |
---|---|---|---|
Solubility | |||
DMSO | 38.94 | 100 | |
ethanol | 7.79 | 20 |
The following data is based on the product molecular weight 389.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 2.57 mL | 12.84 mL | 25.68 mL |
5 mM | 0.51 mL | 2.57 mL | 5.14 mL |
10 mM | 0.26 mL | 1.28 mL | 2.57 mL |
50 mM | 0.05 mL | 0.26 mL | 0.51 mL |
References are publications that support the biological activity of the product.
Yang et al (2014) A novel CXCR4-selective high-affinity fluorescent probe and its application in competitive binding assays. Biochemistry 53 4881 PMID: 25058910
If you know of a relevant reference for FITC, please let us know.
Keywords: FITC, FITC supplier, fluorescent, reagents, labeling, amines, Fluorescein-5-Isothiocyanate, Isomer1, dyes, FITC, Isomer, 1, Standard, Fluorescein,, Coumarin, and, Rhodamine, Dyes, Flow, Cytometry, 5440, Tocris Bioscience
Citations are publications that use Tocris products. Selected citations for FITC include:
Meyer et al (2012) Lipidic nanocapsule drug delivery: neuronal protection for cochlear implant optimization. Int J Nanomedicine 7 2449 PMID: 22654517
Do you know of a great paper that uses FITC from Tocris? Please let us know.
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Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes