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FITC

Cat. No. 5440 1 Citation Submit a Review Datasheet / COA / SDS
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Cat.No. 5440 - FITC | C21H11NO5S | CAS No. 3326-32-7
Description: Green fluorescent dye; supplied as NHS ester
Alternative Names: Fluorescein-5-Isothiocyanate, FITC Isomer 1
Chemical Name: 3',6'-Dihydroxy-5-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Reviews
Literature
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity

Fluorescent reagent for the labeling of amines. Excitation maximum ~ 495 nm; emission maximum ~ 525 nm.

Technical Data

M. Wt 389.38
Formula C21H11NO5S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 3326-32-7
PubChem ID 18730
InChI Key MHMNJMPURVTYEJ-UHFFFAOYSA-N
Smiles OC1=CC=C(C2(C(C=CC(N=C=S)=C3)=C3C(O2)=O)C(C=CC(O)=C4)=C4O5)C5=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
ethanol 7.79 20
DMSO 38.94 100

Preparing Stock Solutions

The following data is based on the product molecular weight 389.38. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.57 mL 12.84 mL 25.68 mL
5 mM 0.51 mL 2.57 mL 5.14 mL
10 mM 0.26 mL 1.28 mL 2.57 mL
50 mM 0.05 mL 0.26 mL 0.51 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Dilution Calculator

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References

References are publications that support the biological activity of the product.

Yang et al (2014) A novel CXCR4-selective high-affinity fluorescent probe and its application in competitive binding assays. Biochemistry 53 4881 PMID: 25058910

If you know of a relevant reference for FITC, please let us know.

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1 Citation for FITC

Citations are publications that use Tocris products. Selected citations for FITC include:

Meyer et al (2012) Lipidic nanocapsule drug delivery: neuronal protection for cochlear implant optimization. Int J Nanomedicine 7 2449 PMID: 22654517

Do you know of a great paper that uses FITC from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

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