Home / Product Type / Fluorescence Imaging / Fluorescent Dyes / Standard Fluorescein, Coumarin and Rhodamine Dyes / FITC

FITC

Cat. No. 5440 1 Citation Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Cat.No. 5440 - FITC | C21H11NO5S | CAS No. 3326-32-7
Description: Green fluorescent dye
Alternative Names: Fluorescein-5-Isothiocyanate, FITC Isomer 1
Chemical Name: 3',6'-Dihydroxy-5-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Reviews
Literature (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for FITC

FITC is a fluorescent reagent for the labeling of amines. Excitation maximum ~ 495 nm; emission maximum ~ 525 nm.

Optical Data for FITC

Emission Color Green
Brightness
λabs 495 nm
λem 525 nm
Extinction Coefficient (ε) 75000 M-1cm-1
Quantum Yield (φ) 0.92
Closest Laser line 488 nm
Reactive Group Isothiocyanate
Reactivity Primary amines
Cell Permeable Yes

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data for FITC

M. Wt 389.38
Formula C21H11NO5S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 3326-32-7
PubChem ID 18730
InChI Key MHMNJMPURVTYEJ-UHFFFAOYSA-N
Smiles OC1=CC=C(C2(C(C=CC(N=C=S)=C3)=C3C(O2)=O)C(C=CC(O)=C4)=C4O5)C5=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for FITC

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 38.94 100
ethanol 7.79 20

Preparing Stock Solutions for FITC

The following data is based on the product molecular weight 389.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.57 mL 12.84 mL 25.68 mL
5 mM 0.51 mL 2.57 mL 5.14 mL
10 mM 0.26 mL 1.28 mL 2.57 mL
50 mM 0.05 mL 0.26 mL 0.51 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References for FITC

References are publications that support the biological activity of the product.

Yang et al (2014) A novel CXCR4-selective high-affinity fluorescent probe and its application in competitive binding assays. Biochemistry 53 4881 PMID: 25058910

If you know of a relevant reference for FITC, please let us know.

View Related Products by Target

Keywords: FITC, FITC supplier, fluorescent, reagents, labeling, amines, Fluorescein-5-Isothiocyanate, Isomer1, dyes, FITC, Isomer, 1, Standard, Fluorescein,, Coumarin, and, Rhodamine, Dyes, Flow, Cytometry, 5440, Tocris Bioscience

1 Citation for FITC

Citations are publications that use Tocris products. Selected citations for FITC include:

Meyer et al (2012) Lipidic nanocapsule drug delivery: neuronal protection for cochlear implant optimization. Int J Nanomedicine 7 2449 PMID: 22654517

Do you know of a great paper that uses FITC from Tocris? Please let us know.

Reviews for FITC

There are currently no reviews for this product. Be the first to review FITC and earn rewards!

Have you used FITC?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Probes and Dyes

Fluorescent Probes and Dyes Research Product Guide

This product guide provides a comprehensive list and background to the use of Fluorescent Probes and Dyes

  • Fluorescent Dyes
  • Dyes for Flow Cytometry
  • Fluorescent Probes
  • Anti-fade Reagents
  • Bioluminescent Substrates
  • Fluorogenic Dyes for Light-Up Aptamers
  • Fluorescent Probes for Imaging Bacteria
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals