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Cat.No. 5440 - FITC | C21H11NO5S | CAS No. 3326-32-7
Description: Green fluorescent dye
Alternative Names: Fluorescein-5-Isothiocyanate, FITC Isomer 1
Chemical Name: 3',6'-Dihydroxy-5-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
Purity: ≥98% (HPLC)
Citations (1)
Literature (1)

Biological Activity for FITC

FITC is a fluorescent reagent for the labeling of amines. Excitation maximum ~ 495 nm; emission maximum ~ 525 nm.

Optical Data for FITC

Emission Color Green
λabs 495 nm
λem 525 nm
Extinction Coefficient (ε) 75000 M-1cm-1
Quantum Yield (φ) 0.92
Closest Laser line 488 nm
Reactive Group Isothiocyanate
Reactivity Primary amines
Cell Permeable Yes

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

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Technical Data for FITC

M. Wt 389.38
Formula C21H11NO5S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 3326-32-7
PubChem ID 18730
Smiles OC1=CC=C(C2(C(C=CC(N=C=S)=C3)=C3C(O2)=O)C(C=CC(O)=C4)=C4O5)C5=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for FITC

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 38.94 100
ethanol 7.79 20

Preparing Stock Solutions for FITC

The following data is based on the product molecular weight 389.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.57 mL 12.84 mL 25.68 mL
5 mM 0.51 mL 2.57 mL 5.14 mL
10 mM 0.26 mL 1.28 mL 2.57 mL
50 mM 0.05 mL 0.26 mL 0.51 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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References for FITC

References are publications that support the biological activity of the product.

Yang et al (2014) A novel CXCR4-selective high-affinity fluorescent probe and its application in competitive binding assays. Biochemistry 53 4881 PMID: 25058910

If you know of a relevant reference for FITC, please let us know.

Keywords: FITC, FITC supplier, fluorescent, reagents, labeling, amines, Fluorescein-5-Isothiocyanate, Isomer1, dyes, FITC, Isomer, 1, Standard, Fluorescein,, Coumarin, and, Rhodamine, Dyes, Flow, Cytometry, 5440, Tocris Bioscience

1 Citation for FITC

Citations are publications that use Tocris products. Selected citations for FITC include:

Meyer et al (2012) Lipidic nanocapsule drug delivery: neuronal protection for cochlear implant optimization. Int J Nanomedicine 7 2449 PMID: 22654517

Do you know of a great paper that uses FITC from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

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