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FITC

Cat. No. 5440 1 Citation COA / Product Datasheet SDS
Pricing Availability Delivery Time Qty
Cat.No. 5440 - FITC | C21H11NO5S | CAS No. 3326-32-7
Description: Fluorescent reagent for the labeling of amines
Alternative Names: Fluorescein-5-Isothiocyanate, FITC Isomer 1
Chemical Name: 3',6'-Dihydroxy-5-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Literature
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity

Fluorescent reagent for the labeling of amines. Excitation maximum ~ 495 nm; emission maximum ~ 525 nm.

Technical Data

M. Wt 389.38
Formula C21H11NO5S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 3326-32-7
PubChem ID 18730
InChI Key MHMNJMPURVTYEJ-UHFFFAOYSA-N
Smiles OC1=CC=C(C2(C(C=CC(N=C=S)=C3)=C3C(O2)=O)C(C=CC(O)=C4)=C4O5)C5=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 38.94 100
ethanol 7.79 20

Preparing Stock Solutions

The following data is based on the product molecular weight 389.38. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.57 mL 12.84 mL 25.68 mL
5 mM 0.51 mL 2.57 mL 5.14 mL
10 mM 0.26 mL 1.28 mL 2.57 mL
50 mM 0.05 mL 0.26 mL 0.51 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Dilution Calculator

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Product Datasheets

Certificate of Analysis / Product Datasheet
Safety Datasheet

References

References are publications that support the products' biological activity.

Yang et al (2014) A novel CXCR4-selective high-affinity fluorescent probe and its application in competitive binding assays. Biochemistry 53 4881 PMID: 25058910

If you know of a relevant reference for FITC, please let us know.

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Keywords: FITC, supplier, fluorescent, reagents, labeling, amines, Fluorescein-5-Isothiocyanate, Isomer1, Fluorescein-5-Isothiocyanate, FITC, Isomer, 1, Fluorescent, Dyes, Tocris Bioscience

1 Citation for FITC

Citations are publications that use Tocris products. Selected citations for FITC include:

Meyer et al (2012) Lipidic nanocapsule drug delivery: neuronal protection for cochlear implant optimization. Int J Nanomedicine 7 2449 PMID: 22654517

Do you know of a great paper that uses FITC from Tocris? If so please let us know.

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New Product Guide (Spring/Summer 2017)

Our new product guide highlights over 130 new products added to the Tocris Bioscience range during the first half of 2017.

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