S-Trityl-L-cysteine

Pricing Availability   Qty
Cat.No. 2191 - S-Trityl-L-cysteine | C22H21NO2S | CAS No. 2799-07-7
Description: Potent, selective inhibitor of mitotic kinesin Eg5
Alternative Names: NSC 83265
Chemical Name: S-(Triphenylmethyl)-L-cysteine
Datasheet
Citations (6)
Reviews (1)
Literature (1)

Biological Activity

Potent, cell-permeable, selective inhibitor of mitotic kinesin Eg5, a protein required for establishing and maintaining a bipolar spindle. Inhibits basal ATPase activity (IC50 = 1 mM) and microtubule-activated ATPase activity of Eg5 (IC50 = 140 nM). Induces mitotic arrest in HeLa cells with an IC50 of 700 nM. Displays antitumor activity.

Technical Data

M. Wt 363.47
Formula C22H21NO2S
Storage Store at +4°C
CAS Number 2799-07-7
PubChem ID 7271795
InChI Key DLMYFMLKORXJPO-HXUWFJFHSA-N
Smiles N[C@@H](C(O)=O)CSC(C2=CC=CC=C2)(C3=CC=CC=C3)C1=CC=CC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 18.17 50

Preparing Stock Solutions

The following data is based on the product molecular weight 363.47. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 5.5 mL 27.51 mL 55.03 mL
2.5 mM 1.1 mL 5.5 mL 11.01 mL
5 mM 0.55 mL 2.75 mL 5.5 mL
25 mM 0.11 mL 0.55 mL 1.1 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References

References are publications that support the biological activity of the product.

DeBonis et al (2004) In vitro screening for inhibitors of the human mitotic kinesin Eg5 with antimitotic and antitumour activities. Mol.Cancer Ther. 3 1079 PMID: 15367702

Brier et al (2004) Identification of the protein binding region of S-trityl-L-cysteine, a new potent inhibitor of mitotic kinesin Eg5. Biochemistry 43 13072 PMID: 15476401


If you know of a relevant reference for S-Trityl-L-cysteine, please let us know.

View Related Products by Product Action

View all Kinesin Inhibitors

Keywords: S-Trityl-L-cysteine, S-Trityl-L-cysteine supplier, Potent, selective, inhibitors, inhibits, mitotic, kinesin, Eg5, Mitotic, Kinesin, Mitosis, NSC83265, NSC, 83265, 2191, Tocris Bioscience

6 Citations for S-Trityl-L-cysteine

Citations are publications that use Tocris products. Selected citations for S-Trityl-L-cysteine include:

Cilibrasi et al (2019) A Ploidy Increase Promotes Sensitivity of Glioma Stem Cells to Aurora Kinases Inhibition. J Oncol 2019 9014045 PMID: 31531022

Ferreira et al (2018) Dissecting the role of the tubulin code in mitosis. Methods Cell Biol 144 33 PMID: 29804676

Hindriksen et al (2017) Baculoviral delivery of CRISPR/Cas9 facilitates efficient genome editing in human cells. PLoS One 12 e0179514 PMID: 28640891

Hengeveld (2017) Inner centromere localization of the CPC maintains centromere cohesion and allows mitoticcheckpoint silencing. Nat Commun 8 15542 PMID: 28561035

Müllers et al (2014) Nuclear translocation of Cyclin B1 marks the restriction point for terminal cell cycle exit in G2 phase. PLoS Genet 13 2733 PMID: 25486360

Hégarat et al (2014) PP2A/B55 and Fcp1 regulate Greatwall and Ensa dephosphorylation during mitotic exit. J Pharmacol Exp Ther 10 e1004004 PMID: 24391510


Do you know of a great paper that uses S-Trityl-L-cysteine from Tocris? Please let us know.

Reviews for S-Trityl-L-cysteine

Average Rating: 4 (Based on 1 Review.)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used S-Trityl-L-cysteine?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


STLC block cells at mitotic entry.
By Valeria Marotta on 10/07/2020
Species: Human
Cell Line/Tissue: U2OS

I used the product to synchronise the cells at mitotic entry.

review image

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis