RSVA 405

Pricing Availability Delivery Time Qty
Cat.No. 5138 - RSVA 405 | C17H20N4O2 | CAS No. 140405-36-3
Description: AMPK activator; also STAT3 inhibitor
Chemical Name: 2-[[4-(Diethylamino)-2-hydroxyphenyl]methylene]hydrazide-4-pyridinecarboxylic acid
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature
Pathways

Biological Activity

AMP-activated protein kinase (AMPK) activator (EC50 = 1 μM); activates AMPK indirectly through a CaMKKβ-dependent mechanism. Also inhibits LPS-induced STAT3 phosphorylation (IC50 = 0.5 μM). Induces autophagy and promotes Aβ degradation in vitro. Also inhibits adipogenesis in vitro and reduces weight gain in diet-induced obese mice.

Technical Data

M. Wt 312.37
Formula C17H20N4O2
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 140405-36-3
PubChem ID 9613337
InChI Key GWQPCBPAOAFXSJ-XDHOZWIPSA-N
Smiles O=C(N/N=C/C2=C(O)C=C(N(CC)CC)C=C2)C1=CC=NC=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 15.62 50
ethanol 1.56 5

Preparing Stock Solutions

The following data is based on the product molecular weight 312.37. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.2 mL 16.01 mL 32.01 mL
5 mM 0.64 mL 3.2 mL 6.4 mL
10 mM 0.32 mL 1.6 mL 3.2 mL
50 mM 0.06 mL 0.32 mL 0.64 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Vingtdeux et al (2011) Novel synthetic small-molecule activators of AMPK as enhancers of autophagy and amyloid-beta peptide degradation. FASEB J. 25 219 PMID: 20852062

Vingtdeux et al (2011) Small-molecule activators of AMP-activated protein kinase (AMPK), RSVA314 and RSVA405, inhibit adipogenesis. Mol Med 17 1022 PMID: 21647536

Capiralla et al (2012) Identification of potent small-molecule inhibitors of STAT3 with anti-inflammatory properties in RAW 264.7 macrophages. FASEB J. 279 3791 PMID: 22909341


If you know of a relevant reference for RSVA 405, please let us know.

View Related Products by Product Action

View all AMPK Activators

Keywords: RSVA405 AMP-activated protein kinases activators inhibitors STAT3 indirect Ab Abeta Aβ amyloid metabolism nf-kb nf-κb cytokines inhibits AMPK

Citations for RSVA 405

Citations are publications that use Tocris products.

Currently there are no citations for RSVA 405. Do you know of a great paper that uses RSVA 405 from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

Kinases

Kinases Product Listing

A collection of over 400 products for kinase research, the listing includes inhibitors of:

  • Receptor Tyrosine Kinases
  • Protein Kinases A, C, D and G
  • PI-3 Kinase, Akt and mTOR
  • MAPK Signaling
  • Receptor Serine/Threonine Kinases

Pathways for RSVA 405

AMPK

AMPK Signaling Pathway

AMPK signaling pathway plays an important role in the cellular reponse to low levels of available ATP, often caused by stresses such as heat shock, ischemia or hypoxia.

Protocols

TODO: Add Protocols