Ro 3306

Pricing Availability   Qty
Description: Cdk1 inhibitor
Chemical Name: 5-(6-Quinolinylmethylene)-2-[(2-thienylmethyl)amino]-4(5H)-thiazolone
Purity: ≥98% (HPLC)
Datasheet
Citations (20)
Reviews

Biological Activity for Ro 3306

Ro 3306 is an ATP-competitive cyclin-dependent kinase 1 (cdk1) inhibitor (Ki values range from 35 to 240 nM depending on cdk1 binding partner). Also reported to inhibit other cdks (Ki values are 0.89-1.32, 0.03 and >2 μM for cdk2, cdk3 and cdk4, respectively). Induces G2/M phase cell cycle arrest and apoptosis. Downregulates the expression of antiapoptotic proteins such as Bcl-2 and survivin and enhances downstream p53 signaling in acute myeloid leukemia (AML). RO 3306 also improves homology-directed repair (HDR) -mediated gene editing in hematopoietic stem and progenitor cells.

Compound Libraries for Ro 3306

Ro 3306 is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for Ro 3306

M. Wt 351.45
Formula C18H13N3OS2
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 872573-93-8
PubChem ID 11631681
InChI Key XOLMRFUGOINFDQ-YBEGLDIGSA-N
Smiles O=C2/C(SC(NCC3=CC=CS3)=N2)=C/C1=CC=C(N=CC=C4)C4=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Ro 3306

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.03 20 with gentle warming

Preparing Stock Solutions for Ro 3306

The following data is based on the product molecular weight 351.45. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 14.23 mL 71.13 mL 142.27 mL
1 mM 2.85 mL 14.23 mL 28.45 mL
2 mM 1.42 mL 7.11 mL 14.23 mL
10 mM 0.28 mL 1.42 mL 2.85 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets for Ro 3306

Certificate of Analysis / Product Datasheet
Select another batch:

References for Ro 3306

References are publications that support the biological activity of the product.

Kojima et al (2009) The CDK1 inhibitor RO-3306 enhances p53-mediated Bax activation and mitochondrial apoptosis in AML. Cancer Sci. 100 1128 PMID: 19385969

Vassilev et al (2006) Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1. Proc.Natl.Acad.Sci. 103 10660 PMID: 16818887

Yu et al (2012) CDK1 regulates mediator of DNA damage checkpoint 1 during mitotic DNA damage. Cancer Res. 72 5448 PMID: 22962268

Jorda et al (2018) How selective are pharmacological inhibitors of cell-cycle-regulating cyclin-dependent kinases? J.Med.Chem. 61 9105 PMID: 30234987

Azhagiri et al (2021) Homology-directed gene-editing approaches for hematopoietic stem and progenitor cell gene therapy. Stem Cell Res.Ther. 12 500 PMID: 34503562


If you know of a relevant reference for Ro 3306, please let us know.

View Related Products by Product Action

View all CDK1 Subfamily Inhibitors

Keywords: Ro 3306, Ro 3306 supplier, Ro3306, inhibits, cdk1, cyclin-dependent, kinase, 1, inhibitors, cell, cycle, arrest, apoptotic, hematopoietic, haematopoietic, stem, cells, HSC, HDR, homology, directed, repair, CRISPR, Cyclin-dependent, Kinase, CDK1, Subfamily, Hematopoietic, Stem, Cells, Reagents, 4181, Tocris Bioscience

20 Citations for Ro 3306

Citations are publications that use Tocris products. Selected citations for Ro 3306 include:

Minsoo et al (2020) Cyclin-Dependent Kinase 5 Inhibitor Butyrolactone I Elicits a Partial Agonist Activity of Peroxisome Proliferator-Activated Receptor γ. Biomolecules 10 PMID: 32054125

Dong et al (2020) Prolonged mitosis results in structurally aberrant and over-elongated centrioles. J Cell Biol 219 PMID: 32271878

Bas et al (2020) A Biosensor for the Mitotic Kinase MPS1 Reveals Spatiotemporal Activity Dynamics and Regulation. Curr Biol 30 3862-3870.e6 PMID: 32888483

Shibani et al (2020) Dysfunctional telomeres trigger cellular senescence mediated by cyclic GMP-AMP synthase. J Biol Chem 295 11144-11160 PMID: 32540968

J et al (2020) The E3 ubiquitin ligase TRIP12 participates in cell cycle progression and chromosome stability. Sci Rep 10 789 PMID: 31964993

Meyer et al (2019) Phosphorylation of MCPH1 isoforms during mitosis followed by isoform-specific degradation by APC/C-CDH1. FASEB J 33 2796 PMID: 30303738

Liccardi et al (2019) RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation. Mol Cell 73 413 PMID: 30598363

Silva et al (2015) A delay prior to mitotic entry triggers caspase 8-dependent cell death in p53-deficient Hela and HCT-116 cells. Mol Cell 14 1070 PMID: 25602147

Goonesekere et al (2014) A meta analysis of pancreatic microarray datasets yields new targets as cancer genes and biomarkers. PLoS One 9 e93046 PMID: 24740004

George et al (2021) MYBL2 and ATM suppress replication stress in pluripotent stem cells. EMBO Rep 22 e51120 PMID: 33779025

Smith et al (2019) PP1 and PP2A Use Opposite Phospho-dependencies to Control Distinct Processes at the Kinetochore. Cell Rep 28 2206 PMID: 31433993

Karki (2017) Precocious centriole disengagement and centrosome fragmentation induced by mitotic delay. Nat Commun 8 15803 PMID: 28607478

Ramkumar et al (2015) JNK-associated Leucine Zipper Protein Functions as a Docking Platform for Polo-like Kinase 1 and Regulation of the Associating Transcription Factor Forkhead Box Protein K1. J Biol Chem 290 29617 PMID: 26468278

Shukla et al (2015) Plk1 relieves centriole block to reduplication by promoting daughter centriole maturation. Nat Commun 6 8077 PMID: 26293378

Junbin et al (2017) An Attachment-Independent Biochemical Timer of the Spindle Assembly Checkpoint. Mol Cell 68 715-730.e5 PMID: 29129638

Rossi et al (2013) Regulation of the CRL4(Cdt2) ubiquitin ligase and cell-cycle exit by the SCF(Fbxo11) ubiquitin ligase. Am J Physiol Gastrointest Liver Physiol 49 1159 PMID: 23478441

Katsuhiko et al (2018) Temporal Regulation of ESCO2 Degradation by the MCM Complex, the CUL4-DDB1-VPRBP Complex, and the Anaphase-Promoting Complex. Curr Biol 28 2665-2672.e5 PMID: 30100344

Angel R et al (2013) Multi-phosphorylation of the intrinsically disordered unique domain of c-Src studied by in-cell and real-time NMR spectroscopy. Chembiochem 14 1820-7 PMID: 23744817

Christos et al (2022) CDK4/6 inhibitors induce replication stress to cause long-term cell cycle withdrawal. EMBO J 41 e108599 PMID: 35037284

Sacristan et al (2018) Dynamic kinetochore size regulation promotes microtubule capture and chromosome biorientation in mitosis. Nat Cell Biol 20 800 PMID: 29915359


Do you know of a great paper that uses Ro 3306 from Tocris? Please let us know.

Reviews for Ro 3306

There are currently no reviews for this product. Be the first to review Ro 3306 and earn rewards!

Have you used Ro 3306?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review