Ro 3306

Pricing Availability Delivery Time Qty
Cat.No. 4181 - Ro 3306 | C18H13N3OS2 | CAS No. 872573-93-8
Description: Cyclin-dependent kinase (cdk) 1 inhibitor
Chemical Name: 5-(6-Quinolinylmethylene)-2-[(2-thienylmethyl)amino]-4(5H)-thiazolone
Purity: ≥98% (HPLC)
Datasheet
Citations (5)
Literature

Biological Activity

ATP-competitive, potent cyclin-dependent kinase (cdk) 1 inhibitor (Ki values are 35 and 110 nM for cdk1/cyclin B1 and cdk1/cyclin A respectively). Induces G2/M phase cell cycle arrest and apoptosis. Downregulates the expression of antiapoptotic proteins such as Bcl-2 and survivin and enhances downstream p53 signaling in acute myeloid leukemia (AML).

Compound Libraries

Ro 3306 is also offered as part of the Tocriscreen Plus and Tocriscreen Kinase Inhibitor Toolbox I. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 351.45
Formula C18H13N3OS2
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 872573-93-8
PubChem ID 11631681
InChI Key XOLMRFUGOINFDQ-YBEGLDIGSA-N
Smiles O=C2/C(SC(NCC3=CC=CS3)=N2)=C/C1=CC=C(N=CC=C4)C4=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 17.57 50

Preparing Stock Solutions

The following data is based on the product molecular weight 351.45. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.85 mL 14.23 mL 28.45 mL
5 mM 0.57 mL 2.85 mL 5.69 mL
10 mM 0.28 mL 1.42 mL 2.85 mL
50 mM 0.06 mL 0.28 mL 0.57 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Kojima et al (2009) The CDK1 inhibitor RO-3306 enhances p53-mediated Bax activation and mitochondrial apoptosis in AML. Cancer Sci. 100 1128 PMID: 19385969

Vassilev et al (2006) Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1. Proc.Natl.Acad.Sci. 103 10660 PMID:

Yu et al (2012) CDK1 regulates mediator of DNA damage checkpoint 1 during mitotic DNA damage. Cancer Res. 72 5448 PMID: 22962268


If you know of a relevant reference for Ro 3306, please let us know.

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Keywords: Ro3306 inhibits cdk1 cyclin-dependent kinase 1 inhibitors cell cycle arrest apoptotic Cyclin-dependent Kinase

5 Citations for Ro 3306

Citations are publications that use Tocris products. Selected citations for Ro 3306 include:

Karki (2017) Precocious centriole disengagement and centrosome fragmentation induced by mitotic delay. Nat Commun 8 15803 PMID: 28607478

Shukla et al (2015) Plk1 relieves centriole block to reduplication by promoting daughter centriole maturation. Nat Commun 6 8077 PMID: 26293378

Silva et al (2015) A delay prior to mitotic entry triggers caspase 8-dependent cell death in p53-deficient Hela and HCT-116 cells. Mol Cell 14 1070 PMID: 25602147

Goonesekere et al (2014) A meta analysis of pancreatic microarray datasets yields new targets as cancer genes and biomarkers. PLoS One 9 e93046 PMID: 24740004

Rossi et al (2013) Regulation of the CRL4(Cdt2) ubiquitin ligase and cell-cycle exit by the SCF(Fbxo11) ubiquitin ligase. Am J Physiol Gastrointest Liver Physiol 49 1159 PMID: 23478441


Do you know of a great paper that uses Ro 3306 from Tocris? If so please let us know.

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Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

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  • Epigenetics in Cancer
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Kinases

Kinases Product Listing

A collection of over 400 products for kinase research, the listing includes inhibitors of:

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  • Protein Kinases A, C, D and G
  • PI-3 Kinase, Akt and mTOR
  • MAPK Signaling
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Neurodegeneration

Neurodegeneration Product Guide

A collection of over 275 products for neurodegeneration research, the guide includes research tools for the study of:

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Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Pathways for Ro 3306

Protocols

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