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ATP-competitive cyclin-dependent kinase 1 (cdk1) inhibitor (Ki values range from 35 to 240 nM depending on cdk1 binding partner). Also reported to inhibit other cdks (Ki values are 0.89-1.32, 0.03 and >2 μM for cdk2, cdk3 and cdk4, respectively). Induces G2/M phase cell cycle arrest and apoptosis. Downregulates the expression of antiapoptotic proteins such as Bcl-2 and survivin and enhances downstream p53 signaling in acute myeloid leukemia (AML).
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
|DMSO||7.03||20 with gentle warming|
Preparing Stock Solutions
The following data is based on the product molecular weight 351.45. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.2 mM||14.23 mL||71.13 mL||142.27 mL|
|1 mM||2.85 mL||14.23 mL||28.45 mL|
|2 mM||1.42 mL||7.11 mL||14.23 mL|
|10 mM||0.28 mL||1.42 mL||2.85 mL|
References are publications that support the biological activity of the product.
Kojima et al (2009) The CDK1 inhibitor RO-3306 enhances p53-mediated Bax activation and mitochondrial apoptosis in AML. Cancer Sci. 100 1128 PMID: 19385969
Vassilev et al (2006) Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1. Proc.Natl.Acad.Sci. 103 10660 PMID: 16818887
Yu et al (2012) CDK1 regulates mediator of DNA damage checkpoint 1 during mitotic DNA damage. Cancer Res. 72 5448 PMID: 22962268
Jorda et al (2018) How selective are pharmacological inhibitors of cell-cycle-regulating cyclin-dependent kinases? J.Med.Chem. 61 9105 PMID: 30234987
If you know of a relevant reference for Ro 3306, please let us know.
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Keywords: Ro 3306, Ro 3306 supplier, Ro3306, inhibits, cdk1, cyclin-dependent, kinase, 1, inhibitors, cell, cycle, arrest, apoptotic, Cyclin-dependent, Kinase, 4181, Tocris Bioscience
10 Citations for Ro 3306
Citations are publications that use Tocris products. Selected citations for Ro 3306 include:
Smith et al (2019) PP1 and PP2A Use Opposite Phospho-dependencies to Control Distinct Processes at the Kinetochore. Cell Rep 28 2206 PMID: 31433993
Karki (2017) Precocious centriole disengagement and centrosome fragmentation induced by mitotic delay. Nat Commun 8 15803 PMID: 28607478
Liccardi et al (2019) RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation. Mol Cell 73 413 PMID: 30598363
Meyer et al (2019) Phosphorylation of MCPH1 isoforms during mitosis followed by isoform-specific degradation by APC/C-CDH1. FASEB J 33 2796 PMID: 30303738
Rossi et al (2013) Regulation of the CRL4(Cdt2) ubiquitin ligase and cell-cycle exit by the SCF(Fbxo11) ubiquitin ligase. Am J Physiol Gastrointest Liver Physiol 49 1159 PMID: 23478441
Shukla et al (2015) Plk1 relieves centriole block to reduplication by promoting daughter centriole maturation. Nat Commun 6 8077 PMID: 26293378
Ramkumar et al (2015) JNK-associated Leucine Zipper Protein Functions as a Docking Platform for Polo-like Kinase 1 and Regulation of the Associating Transcription Factor Forkhead Box Protein K1. J Biol Chem 290 29617 PMID: 26468278
Sacristan et al (2018) Dynamic kinetochore size regulation promotes microtubule capture and chromosome biorientation in mitosis. Nat Cell Biol 20 800 PMID: 29915359
Goonesekere et al (2014) A meta analysis of pancreatic microarray datasets yields new targets as cancer genes and biomarkers. PLoS One 9 e93046 PMID: 24740004
Silva et al (2015) A delay prior to mitotic entry triggers caspase 8-dependent cell death in p53-deficient Hela and HCT-116 cells. Mol Cell 14 1070 PMID: 25602147
Do you know of a great paper that uses Ro 3306 from Tocris? Please let us know.
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Literature in this Area
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Cell Cycle & DNA Damage Repair Poster
In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.