Ro 3280

Pricing Availability Delivery Time Qty
Cat.No. 5968 - Ro 3280 | C27H35F2N7O3 | CAS No. 1062243-51-9
Description: Potent and selective PLK1 inhibitor
Chemical Name: 4-[(9-Cyclopentyl-7,7-difluoro-6,7,8,9-tetrahydro-5-methyl-6-oxo-5H-pyrimido[4,5-b][1,4]diazepin-2-yl)amino]-3-methoxy-N-(1-methyl-4-piperidinyl)benzamide
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Potent and selective PLK inhibitor (IC50 = 3 nM). Exhibits selectivity for PLK1 over a panel of other kinases. Inhibits growth of a range of tumor cells lines in vitro and induces apoptosis in leukemia cells. Exhibits antitumor activity in mouse colorectal and nasopharyngeal cancer models in vivo.

Technical Data

M. Wt 543.61
Formula C27H35F2N7O3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1062243-51-9
PubChem ID 25015677
InChI Key DJNZZLZKAXGMMC-UHFFFAOYSA-N
Smiles COC1=C(C=CC(C(NC2CCN(CC2)C)=O)=C1)NC3=NC=C4N(C(C(F)(CN(C4=N3)C5CCCC5)F)=O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 27.18 50
ethanol 27.18 50

Preparing Stock Solutions

The following data is based on the product molecular weight 543.61. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.84 mL 9.2 mL 18.4 mL
5 mM 0.37 mL 1.84 mL 3.68 mL
10 mM 0.18 mL 0.92 mL 1.84 mL
50 mM 0.04 mL 0.18 mL 0.37 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Cheung et al (2013) Polo-like kinase inhibitor Ro5203280 has potent antitumor activity in nasopharyngeal carcinoma. Mol.Cancer Ther. 12 1393 PMID: 23686835

Chen et al (2012) Identification of novel, potent and selective inhibitors of Polo-like kinase 1. Bioorg.Med.Chem.Lett. 22 1247 PMID: 22172702

Wang et al (2015) Molecular targeting of the oncoprotein PLK1 in pediatric acute myeloid leukemia: RO3280, a novel PLK1 inhibitor, induces apoptosis in leukemia cells. Int.J.Mol.Sci. 16 1266 PMID: 25574601


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Keywords: Ro 3280, supplier, Ro3280, Polo-like, kinases, inhibitors, inhibits, apoptosis, inducers, anticancer, PLK1, potent, selective, Polo-like, Kinase, Tocris Bioscience

Citations for Ro 3280

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Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Kinases

Kinases Product Listing

A collection of over 400 products for kinase research, the listing includes inhibitors of:

  • Receptor Tyrosine Kinases
  • Protein Kinases A, C, D and G
  • PI-3 Kinase, Akt and mTOR
  • MAPK Signaling
  • Receptor Serine/Threonine Kinases
Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Pathways for Ro 3280

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