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ProK is a lysine-based unnatural amino acid (UAA) used for genetic code expansion. It enables bioconjugation of fluorescent probes in E. coli or multicellular organisms.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
The following data is based on the product molecular weight 264.71. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||3.78 mL||18.89 mL||37.78 mL|
|5 mM||0.76 mL||3.78 mL||7.56 mL|
|10 mM||0.38 mL||1.89 mL||3.78 mL|
|50 mM||0.08 mL||0.38 mL||0.76 mL|
References are publications that support the biological activity of the product.
Nguyen et al (2009) Genetic encoding and labeling of aliphatic azides and alkynes in recombinant proteins via a pyrrolysyl-tRNA Synthetase/tRNA(CUA) pair and click chemistry. J.Am.Chem.Soc. 131 8720 PMID: 19514718
Neumann et al (2010) Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome. Nature 464 441 PMID: 20154731
Li et al (2014) Palladium-triggered deprotection chemistry for protein activation in living cells. Nat. Chem. 6 352 PMID: 24651204
Greiss et al (2011) Expanding the genetic code of an animal. J.Am.Chem.Soc. 133 14196 PMID: 21819153
Meineke et al (2020) Site-specific incorporation of two ncAAs for two-color bioorthogonal labeling and crosslinking of proteins on live mammalian cells. Cell Rep. 31 107811 PMID: 32579937
If you know of a relevant reference for ProK, please let us know.
Keywords: ProK, ProK supplier, unnatural, amino, acid, protein, non-canonical, tagging, labeling, labelling, UAA, Amino, Acids, Unnatural, 7361, Tocris Bioscience
Citations are publications that use Tocris products.
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This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our: