PRIMA-1MET

Pricing Availability Delivery Time Qty
Cat.No. 3710 - PRIMA-1MET | C10H17NO3 | CAS No. 5291-32-7
Description: Restores mutant p53 activity
Alternative Names: APR-246
Chemical Name: 2-(Hydroxymethyl)-2-(methoxymethyl)-1-azabicyclo[2.2.2]octan-3-one
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Literature
Pathways

Biological Activity

Methylated derivative of PRIMA-1 (Cat. No. 1862). Restores mutant activity p53 activity. Acts synergistically with chemotherapeutic agents to inhibit tumor cell proliferation.

Technical Data

M. Wt 199.25
Formula C10H17NO3
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 5291-32-7
PubChem ID 52918385
InChI Key BGBNULCRKBVAKL-UHFFFAOYSA-N
Smiles OCC1(COC)C(C2CCN1CC2)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 19.93 100
water 19.93 100

Preparing Stock Solutions

The following data is based on the product molecular weight 199.25. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.02 mL 25.09 mL 50.19 mL
5 mM 1 mL 5.02 mL 10.04 mL
10 mM 0.5 mL 2.51 mL 5.02 mL
50 mM 0.1 mL 0.5 mL 1 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References

References are publications that support the products' biological activity.

Bykov et al (2005) PRIMA-1MET synergizes with cisplatin to induce tumor cell apoptosis. Oncogene 24 3484 PMID: 15735745

Shen et al (2008) PRIMA-1MET induces mitochondrial apoptosis through activation of caspase-2. Oncogene 27 6571 PMID: 18663359

Stuber et al (2009) PRIMA-1MET induces nucleolar translocation of Epstein-Barr virus-encoded EBNA-5 protein. Mol.Cancer 8 23 PMID: 19323829


If you know of a relevant reference for PRIMA-1MET, please let us know.

View Related Products by Target

Keywords: Restores mutant p53 activity transcription factors APR-246 APR-246 p53

1 Citation for PRIMA-1MET

Citations are publications that use Tocris products. Selected citations for PRIMA-1MET include:

Robinson et al (2013) RB1 status in triple negative breast cancer cells dictates response to radiation treatment and selective therapeutic drugs. PLoS One 8 e78641 PMID: 24265703


Do you know of a great paper that uses PRIMA-1MET from Tocris? If so please let us know.

Commented out for usability testing

Reviews

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Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Pathways for PRIMA-1MET

p53

p53 Signaling Pathway

p53 signaling plays an important role in the co-ordination of the cellular response different types of stress such as DNA damage and hypoxia. The downstream signals lead to apoptosis, senescence and cell cycle arrest.

Protocols

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