Methylated derivative of PRIMA-1 (Cat. No. 1862). Restores mutant activity p53 activity. Acts synergistically with chemotherapeutic agents to inhibit tumor cell proliferation.
|Storage||Store at +4°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 199.25. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||5.02 mL||25.09 mL||50.19 mL|
|5 mM||1 mL||5.02 mL||10.04 mL|
|10 mM||0.5 mL||2.51 mL||5.02 mL|
|50 mM||0.1 mL||0.5 mL||1 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Bykov et al (2005) PRIMA-1MET synergizes with cisplatin to induce tumor cell apoptosis. Oncogene 24 3484 PMID: 15735745
Shen et al (2008) PRIMA-1MET induces mitochondrial apoptosis through activation of caspase-2. Oncogene 27 6571 PMID: 18663359
Stuber et al (2009) PRIMA-1MET induces nucleolar translocation of Epstein-Barr virus-encoded EBNA-5 protein. Mol.Cancer 8 23 PMID: 19323829
If you know of a relevant reference for PRIMA-1MET, please let us know.
Keywords: Restores mutant p53 activity transcription factors APR-246 APR-246 p53
1 Citation for PRIMA-1MET
Citations are publications that use Tocris products. Selected citations for PRIMA-1MET include:
Robinson et al (2013) RB1 status in triple negative breast cancer cells dictates response to radiation treatment and selective therapeutic drugs. PLoS One 8 e78641 PMID: 24265703
Do you know of a great paper that uses PRIMA-1MET from Tocris? If so please let us know.
Literature in this Area
Cell Cycle & DNA Damage Repair Poster
In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.