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Non-selective protease inhibitor; irreversibly sulfonates key active site serine residues in serine proteases, acetylcholinesterases, and thiol proteases. Blocks metabolism of anandamide (Cat. No. 1339) in a rodent model of cannabimimetic activity. Unstable in water (t1/2 ~ 60 mins at pH 7.5). Brain penetrant.
|Storage||Desiccate at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 174.19. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||5.74 mL||28.7 mL||57.41 mL|
|5 mM||1.15 mL||5.74 mL||11.48 mL|
|10 mM||0.57 mL||2.87 mL||5.74 mL|
|50 mM||0.11 mL||0.57 mL||1.15 mL|
References are publications that support the biological activity of the product.
Turini et al (1969) The action of phenylmethylsulfonyl fluoride on human acetylcholinesterase, chymotrypsin and trypsin. J.Pharmacol.Exp.Ther. 167 98 PMID: 4306345
Compton & Martin (1997) The effect of the enzyme inhibitor phenylmethylsulfonyl fluoride on the pharmacological effect of anandamide in the mouse model of cannabimimetic activity. J.Pharmacol.Exp.Ther. 283 1138 PMID: 9399986
Day et al (2001) Role of fatty acid amide hydrolase in the transport of the endogenous cannabinoid anandamide. Mol.Pharmacol. 59 1369 PMID: 11353795
If you know of a relevant reference for PMSF, please let us know.
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Keywords: PMSF, PMSF supplier, proteases, inhibits, inhibitors, irreversible, sulfonation, brain, penetrant, FAAH, anandamide, fatty, acid, amide, hydrolase, Other, Proteases, 4486, Tocris Bioscience
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Used to isolate protein from kidney tissue from wild type or IL-33 deficient mice that underwent cisplatin-induced kidney injury; several proteins were detected by immunoblot analysis; even after several freeze thaws, data has been consistent*additionally added a protease inhibitor cocktail and phosphatase inhibitor cocktail + RIPA for protein isolation
Does not contain phosphatase inhibitors; add these to protease inhibitor mix if analyzing phosphorylated proteins
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