PMSF

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Cat.No. 4486 - PMSF | C7H7FO2S | CAS No. 329-98-6
Description: Non-selective protease inhibitor; also blocks anandamide metabolism
Chemical Name: Phenylmethylsulfonyl fluoride
Purity: ≥98%
Datasheet
Citations
Reviews
Literature

Biological Activity

Non-selective protease inhibitor; irreversibly sulfonates key active site serine residues in serine proteases, acetylcholinesterases, and thiol proteases. Blocks metabolism of anandamide (Cat. No. 1339) in a rodent model of cannabimimetic activity. Unstable in water (t1/2 ~ 60 mins at pH 7.5). Brain penetrant.

Technical Data

M. Wt 174.19
Formula C7H7FO2S
Storage Desiccate at RT
Purity ≥98%
CAS Number 329-98-6
PubChem ID 4784
InChI Key YBYRMVIVWMBXKQ-UHFFFAOYSA-N
Smiles O=S(CC1=CC=CC=C1)(F)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
ethanol 17.42 100
DMSO 17.42 100

Preparing Stock Solutions

The following data is based on the product molecular weight 174.19. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.74 mL 28.7 mL 57.41 mL
5 mM 1.15 mL 5.74 mL 11.48 mL
10 mM 0.57 mL 2.87 mL 5.74 mL
50 mM 0.11 mL 0.57 mL 1.15 mL

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References

References are publications that support the biological activity of the product.

Turini et al (1969) The action of phenylmethylsulfonyl fluoride on human acetylcholinesterase, chymotrypsin and trypsin. J.Pharmacol.Exp.Ther. 167 98 PMID: 4306345

Compton & Martin (1997) The effect of the enzyme inhibitor phenylmethylsulfonyl fluoride on the pharmacological effect of anandamide in the mouse model of cannabimimetic activity. J.Pharmacol.Exp.Ther. 283 1138 PMID: 9399986

Day et al (2001) Role of fatty acid amide hydrolase in the transport of the endogenous cannabinoid anandamide. Mol.Pharmacol. 59 1369 PMID: 11353795


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Keywords: PMSF, PMSF supplier, proteases, inhibits, inhibitors, irreversible, sulfonation, brain, penetrant, FAAH, anandamide, fatty, acid, amide, hydrolase, Other, Proteases, 4486, Tocris Bioscience

Citations for PMSF

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