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Description: Non-selective protease inhibitor; also blocks anandamide metabolism
Chemical Name: Phenylmethylsulfonyl fluoride
Purity: ≥98%
Citations (3)
Reviews (1)

Biological Activity for PMSF

PMSF is a non-selective protease inhibitor; irreversibly sulfonates key active site serine residues in serine proteases, acetylcholinesterases, and thiol proteases. Blocks metabolism of anandamide (Cat. No. 1339) in a rodent model of cannabimimetic activity. Unstable in water (t1/2 ~ 60 mins at pH 7.5). Brain penetrant.

Technical Data for PMSF

M. Wt 174.19
Formula C7H7FO2S
Storage Desiccate at RT
Purity ≥98%
CAS Number 329-98-6
PubChem ID 4784
Smiles O=S(CC1=CC=CC=C1)(F)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for PMSF

Solvent Max Conc. mg/mL Max Conc. mM
ethanol 17.42 100
DMSO 17.42 100

Preparing Stock Solutions for PMSF

The following data is based on the product molecular weight 174.19. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 5.74 mL 28.7 mL 57.41 mL
5 mM 1.15 mL 5.74 mL 11.48 mL
10 mM 0.57 mL 2.87 mL 5.74 mL
50 mM 0.11 mL 0.57 mL 1.15 mL

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References for PMSF

References are publications that support the biological activity of the product.

Turini et al (1969) The action of phenylmethylsulfonyl fluoride on human acetylcholinesterase, chymotrypsin and trypsin. J.Pharmacol.Exp.Ther. 167 98 PMID: 4306345

Compton & Martin (1997) The effect of the enzyme inhibitor phenylmethylsulfonyl fluoride on the pharmacological effect of anandamide in the mouse model of cannabimimetic activity. J.Pharmacol.Exp.Ther. 283 1138 PMID: 9399986

Day et al (2001) Role of fatty acid amide hydrolase in the transport of the endogenous cannabinoid anandamide. Mol.Pharmacol. 59 1369 PMID: 11353795

If you know of a relevant reference for PMSF, please let us know.

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Keywords: PMSF, PMSF supplier, proteases, inhibits, inhibitors, irreversible, sulfonation, brain, penetrant, FAAH, anandamide, fatty, acid, amide, hydrolase, Other, Proteases, 4486, Tocris Bioscience

3 Citations for PMSF

Citations are publications that use Tocris products. Selected citations for PMSF include:

Partha et al (2023) Structural basis of selective cannabinoid CB2 receptor activation. Nat Commun 14 1447 PMID: 36922494

Yan et al (2021) Rapid assessment of DCLK1 inhibitors using a peptide substrate mobility shift assay. STAR Protoc 2 100587 PMID: 34159321

Yingqiong et al (2021) A simple protocol to produce mature human-induced pluripotent stem cell-derived cardiomyocytes. STAR Protoc 2 100912 PMID: 34755117

Do you know of a great paper that uses PMSF from Tocris? Please let us know.

Reviews for PMSF

Average Rating: 4 (Based on 1 Review.)

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Works well for protein isolation.
By Nicole Brown on 01/07/2019
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: Kidney

Used to isolate protein from kidney tissue from wild type or IL-33 deficient mice that underwent cisplatin-induced kidney injury; several proteins were detected by immunoblot analysis; even after several freeze thaws, data has been consistent*additionally added a protease inhibitor cocktail and phosphatase inhibitor cocktail + RIPA for protein isolation

Does not contain phosphatase inhibitors; add these to protease inhibitor mix if analyzing phosphorylated proteins

PMID: 29070568
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