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PIPES, free acid
Buffer. Working pH range in aqueous solution: 6.1 - 7.5. Recommended for use in solutions containing metal ions. Commonly used in cell culture media, protein crystalization and gel eletrophoresis.
|Storage||Store at RT|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 302.4. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|5 mM||0.66 mL||3.31 mL||6.61 mL|
|25 mM||0.13 mL||0.66 mL||1.32 mL|
|50 mM||0.07 mL||0.33 mL||0.66 mL|
|250 mM||0.01 mL||0.07 mL||0.13 mL|
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PIPES [piperazine-N,N-bis(2-ethanesulfonic acid)] buffer was prepared in 18.2 megohms water and filtered through 0.22-micron filter.PH: 6.8 +/- 0.15 after dilution with DI water.
Store at 2-8 degrees C
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