PIPES, free acid

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Cat.No. 5889 - PIPES, free acid | C8H18N2O6S2 | CAS No. 5625-37-6
Description: Commonly used buffer
Chemical Name: Piperazine-N,N'-bis(2-ethanesulfonic acid)
Purity: ≥98%
Reviews (1)
Literature (1)

Biological Activity

Buffer. Working pH range in aqueous solution: 6.1 - 7.5. Recommended for use in solutions containing metal ions. Commonly used in cell culture media, protein crystalization and gel eletrophoresis.

Technical Data

M. Wt 302.4
Formula C8H18N2O6S2
Storage Store at RT
Purity ≥98%
CAS Number 5625-37-6
PubChem ID 79723
Smiles O=S(CCN1CCN(CCS(=O)(O)=O)CC1)(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
1M NaOH 151.2 500

Preparing Stock Solutions

The following data is based on the product molecular weight 302.4. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
5 mM 0.66 mL 3.31 mL 6.61 mL
25 mM 0.13 mL 0.66 mL 1.32 mL
50 mM 0.07 mL 0.33 mL 0.66 mL
250 mM 0.01 mL 0.07 mL 0.13 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Citations for PIPES, free acid

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PIPES [piperazine-N,N-bis(2-ethanesulfonic acid)] buffer was prepared.
By Anonymous on 02/13/2019
Species: Human

PIPES [piperazine-N,N-bis(2-ethanesulfonic acid)] buffer was prepared in 18.2 megohms water and filtered through 0.22-micron filter.PH: 6.8 +/- 0.15 after dilution with DI water.

Store at 2-8 degrees C

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