Ocean Blue, SE

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Cat.No. 6489 - Ocean Blue, SE | C14H7F2NO7 | CAS No. 215868-33-0
Description: Blue fluorescent dye; supplied as NHS ester
Chemical Name: 3-Carboxy-6,8-difluoro-7-hydroxycoumaryl succinimidyl ester
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature

Biological Activity

Blue fluorescent dye. Conjugates of this dye are strongly fluorescent even at neutral pH. Excitation maximum = 405 nm; emission maximum = 455 nm; extinction coefficient = 36000 M-1cm-1; correction factor A280 = 0.15.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

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Technical Data

M. Wt 339.2
Formula C14H7F2NO7
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 215868-33-0
PubChem ID 56927770
InChI Key NZYXABPVYJRICY-UHFFFAOYSA-N
Smiles OC1=C(C=C2C=C(C(OC2=C1F)=O)C(ON3C(CCC3=O)=O)=O)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 3.39 10

Preparing Stock Solutions

The following data is based on the product molecular weight 339.2. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 29.48 mL 147.41 mL 294.81 mL
0.5 mM 5.9 mL 29.48 mL 58.96 mL
1 mM 2.95 mL 14.74 mL 29.48 mL
5 mM 0.59 mL 2.95 mL 5.9 mL

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Reconstitution Calculator

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Dilution Calculator

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References

References are publications that support the biological activity of the product.

Sun et al (1998) Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes. Bioorg.Med.Chem.Lett. 8 3107 PMID: 9873685

Brandtzaeg et al (1998) The increasing power of immunohistochemistry and immunocytochemistry. J.Immunol.Methods 216 49 PMID: 9760215

Haugland et al (2001) Antibody conjugates for cell biology. Curr.Protoc.Cell Biol. PMID: 18228336

Cox et al (2004) Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling. Biotechniques 36 114 PMID: 14740493


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Literature in this Area

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