Ocean Blue, SE

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Description: Blue fluorescent dye (Reactive group: NHS ester). Application: flow cytometry, fluorescence microscopy
Chemical Name: 3-Carboxy-6,8-difluoro-7-hydroxycoumaryl succinimidyl ester
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Protocols (1)
Literature (1)

Biological Activity for Ocean Blue, SE

Key information: Ocean Blue, SE is a blue fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines.

Application: Flow cytometry, fluorescence microscopy.

Properties and Photophysical Data: Conjugates of this dye are strongly fluorescent even at neutral pH. Excitation and emission maxima (λ) are 405 nM and 455 nM, respectively; quantum yield = 0.78; extinction coefficient = 36,000 M-1cm-1; A280 correction factor = 0.15.

Please see the protocol for further information on protein/antibody labeling and conjugation.

Note: This product is typically prepared in DMSO.

Optical Data for Ocean Blue, SE

Emission Color Blue
Brightness
λabs 405 nm
λem 455 nm
Extinction Coefficient (ε) 36000 M-1cm-1
Quantum Yield (φ) 0.78
Closest Laser line 405 nm
Reactive Group NHS ester
Reactivity Primary amines
Correction Factor 280 0.15
Application Flow Cytometry, Fluorescence Microscopy

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

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Technical Data for Ocean Blue, SE

M. Wt 339.2
Formula C14H7F2NO7
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 215868-33-0
PubChem ID 56927770
InChI Key NZYXABPVYJRICY-UHFFFAOYSA-N
Smiles OC1=C(C=C2C=C(C(OC2=C1F)=O)C(ON3C(CCC3=O)=O)=O)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for Ocean Blue, SE

Certificate of Analysis / Product Datasheet
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References for Ocean Blue, SE

References are publications that support the biological activity of the product.

Sun et al (1998) Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes. Bioorg.Med.Chem.Lett. 8 3107 PMID: 9873685

Brandtzaeg et al (1998) The increasing power of immunohistochemistry and immunocytochemistry. J.Immunol.Methods 216 49 PMID: 9760215

Haugland et al (2001) Antibody conjugates for cell biology. Curr.Protoc.Cell Biol. PMID: 18228336

Cox et al (2004) Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling. Biotechniques 36 114 PMID: 14740493


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Keywords: Ocean Blue, SE, Ocean Blue, SE supplier, Blue, fluorescent, dye, Standard, Fluorescein,, Coumarin, and, Rhodamine, Dyes, Flow, Cytometry, 6489, Tocris Bioscience

Citations for Ocean Blue, SE

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Protocols for Ocean Blue, SE

The following protocol features additional information for the use of Ocean Blue, SE (Cat. No. 6489).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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