Ocean Blue, SE

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Cat.No. 6489 - Ocean Blue, SE | C14H7F2NO7 | CAS No. 215868-33-0
Description: Blue fluorescent dye; supplied as NHS ester
Chemical Name: 3-Carboxy-6,8-difluoro-7-hydroxycoumaryl succinimidyl ester
Purity: ≥95% (HPLC)
Protocols (1)
Literature (1)

Biological Activity

Blue fluorescent dye. Conjugates of this dye are strongly fluorescent even at neutral pH. Excitation maximum = 405 nm; emission maximum = 455 nm; extinction coefficient = 36000 M-1cm-1; correction factor A280 = 0.15.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M. Wt 339.2
Formula C14H7F2NO7
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 215868-33-0
PubChem ID 56927770
Smiles OC1=C(C=C2C=C(C(OC2=C1F)=O)C(ON3C(CCC3=O)=O)=O)F

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 3.39 10

Preparing Stock Solutions

The following data is based on the product molecular weight 339.2. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 29.48 mL 147.41 mL 294.81 mL
0.5 mM 5.9 mL 29.48 mL 58.96 mL
1 mM 2.95 mL 14.74 mL 29.48 mL
5 mM 0.59 mL 2.95 mL 5.9 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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References are publications that support the biological activity of the product.

Sun et al (1998) Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes. Bioorg.Med.Chem.Lett. 8 3107 PMID: 9873685

Brandtzaeg et al (1998) The increasing power of immunohistochemistry and immunocytochemistry. J.Immunol.Methods 216 49 PMID: 9760215

Haugland et al (2001) Antibody conjugates for cell biology. Curr.Protoc.Cell Biol. PMID: 18228336

Cox et al (2004) Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling. Biotechniques 36 114 PMID: 14740493

If you know of a relevant reference for Ocean Blue, SE, please let us know.

Keywords: Ocean Blue, SE, Ocean Blue, SE supplier, Blue, fluorescent, dye, Standard, Fluorescein,, Coumarin, and, Rhodamine, Dyes, 6489, Tocris Bioscience

Citations for Ocean Blue, SE

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Protocols for Ocean Blue, SE

The following protocol features additional information for the use of Ocean Blue, SE (Cat. No. 6489).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

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