O4I2

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Cat.No. 5664 - O4I2 | C12H11ClN2O2S | CAS No. 165682-93-9
Description: Oct3/4 inducer; induces expression of pluripotent-associated genes
Chemical Name: Ethyl 2-[(4-chlorophenyl)amino]-4-thiazolecarboxylate
Purity: ≥98% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Oct3/4 inducer; promotes Oct3/4 expression and stabilizes the Oct3/4 protein in HEK293 cells. Induces expression of pluripotent-associated genes Lin28, Sox2 and Nanog, and suppresses Rex1. Induces 15-fold increase in Oct3/4 expression in human primary fibroblasts.

Compound Libraries

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Technical Data

M. Wt 282.75
Formula C12H11ClN2O2S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 165682-93-9
PubChem ID 53428670
InChI Key ULUBAPWNHROTEU-UHFFFAOYSA-N
Smiles ClC(C=C2)=CC=C2NC1=NC(C(OCC)=O)=CS1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 28.27 100
ethanol 14.14 50

Preparing Stock Solutions

The following data is based on the product molecular weight 282.75. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.54 mL 17.68 mL 35.37 mL
5 mM 0.71 mL 3.54 mL 7.07 mL
10 mM 0.35 mL 1.77 mL 3.54 mL
50 mM 0.07 mL 0.35 mL 0.71 mL

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Product Datasheets

Safety Datasheet

References

References are publications that support the products' biological activity.

Cheng et al (2015) Ethyl 2-((4-Chlorophenyl)amino)thiazole-4-carboxylate and derivatives are potent inducers of Oct3/4. J.Med.Chem. 58 5742 PMID: 26143659


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Keywords: O4I2, supplier, octamer, binding, transcription, factors, oct4, inducing, compound, 2, Oct3/4, inducers, pluripotent, stem, cells, induction, Reprogramming, Reprogramming, Tocris Bioscience

Citations for O4I2

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Literature in this Area

Stem Cells

Stem Cells Scientific Review

Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.

Pathways for O4I2

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