O4I2

Pricing Availability   Qty
Cat.No. 5664 - O4I2 | C12H11ClN2O2S | CAS No. 165682-93-9
Description: Oct3/4 inducer; induces expression of pluripotent-associated genes
Chemical Name: Ethyl 2-[(4-chlorophenyl)amino]-4-thiazolecarboxylate
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature

Biological Activity

Oct3/4 inducer; promotes Oct3/4 expression and stabilizes the Oct3/4 protein in HEK293 cells. Induces expression of pluripotent-associated genes Lin28, Sox2 and Nanog, and suppresses Rex1. Induces 15-fold increase in Oct3/4 expression in human primary fibroblasts.

Compound Libraries

O4I2 is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.

Technical Data

M. Wt 282.75
Formula C12H11ClN2O2S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 165682-93-9
PubChem ID 53428670
InChI Key ULUBAPWNHROTEU-UHFFFAOYSA-N
Smiles ClC(C=C2)=CC=C2NC1=NC(C(OCC)=O)=CS1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 28.27 100
ethanol 14.14 50

Preparing Stock Solutions

The following data is based on the product molecular weight 282.75. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.54 mL 17.68 mL 35.37 mL
5 mM 0.71 mL 3.54 mL 7.07 mL
10 mM 0.35 mL 1.77 mL 3.54 mL
50 mM 0.07 mL 0.35 mL 0.71 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References

References are publications that support the biological activity of the product.

Cheng et al (2015) Ethyl 2-((4-Chlorophenyl)amino)thiazole-4-carboxylate and derivatives are potent inducers of Oct3/4. J.Med.Chem. 58 5742 PMID: 26143659


If you know of a relevant reference for O4I2, please let us know.

View Related Products by Target

Keywords: O4I2, O4I2 supplier, octamer, binding, transcription, factors, oct4, inducing, compound, 2, Oct3/4, inducers, pluripotent, stem, cells, induction, Reprogramming, 5664, Tocris Bioscience

Citations for O4I2

Citations are publications that use Tocris products.

Currently there are no citations for O4I2. Do you know of a great paper that uses O4I2 from Tocris? Please let us know.

Reviews for O4I2

There are currently no reviews for this product. Be the first to review O4I2 and earn rewards!

Have you used O4I2?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.


Stem Cells

Stem Cells Scientific Review

Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.

Stem Cells

Stem Cells Poster

Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.