NSC 95397

Pricing Availability Delivery Time Qty
Cat.No. 1547 - NSC 95397 | C14H14O4S2 | CAS No. 93718-83-3
Description: Selective Cdc25 dual specificity phosphatase inhibitor
Chemical Name: 2,3-bis[(2-Hydroxyethyl)thiol]-1,4-naphthoquinone
Purity: ≥97% (HPLC)
Datasheet
Citations
Literature

Biological Activity

Potent and selective irreversible inhibitor of Cdc25 dual specificity phosphatases (Ki values are 32, 96 and 40 nM for inhibition of Cdc25A, -B and -C respectively). Displays 125 - 180-fold selectivity over VH1-related dual-specificity phosphatase and protein tyrosine phosphatase 1b. Inhibits carcinoma cell growth and blocks G2/M phase transition in vitro.

Technical Data

M. Wt 310.38
Formula C14H14O4S2
Storage Desiccate at -20°C
Purity ≥97% (HPLC)
CAS Number 93718-83-3
PubChem ID 262093
InChI Key MAASHDQFQDDECQ-UHFFFAOYSA-N
Smiles OCCSC1=C(SCCO)C(=O)C2=CC=CC=C2C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

All Tocris products are intended for laboratory research use only.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 15.52 50
ethanol 1.55 5

Preparing Stock Solutions

The following data is based on the product molecular weight 310.38. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.22 mL 16.11 mL 32.22 mL
5 mM 0.64 mL 3.22 mL 6.44 mL
10 mM 0.32 mL 1.61 mL 3.22 mL
50 mM 0.06 mL 0.32 mL 0.64 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets

Certificate of Analysis / Product Datasheet
Select another batch:
Safety Datasheet

References

References are publications that support the products' biological activity.

Han et al (2004) NAD(P)H:Quinone oxidoreductase-1-dependent and -independent cytotoxicity of potent quinone Cdc25 phosphatase inhibitors. J.Pharmacol.Exp.Ther. 309 64 PMID: 14718602

Lazo et al (2002) Identification of a potent and selective pharmacophore for Cdc25 dual specificity phosphatase inhibitors. Mol.Pharmacol. 61 720 PMID: 11901209

Melchheier et al (2005) Quinone-induced Cdc25A inhibition causes ERK-dependent connexin phosphorylation. Biochem.Biophys.Res.Commun. 327 1016 PMID: 15652497


If you know of a relevant reference for NSC 95397, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Cdc25 Phosphatase Inhibitors

Keywords: Selective Cdc25 dual specificity phosphatases inhibitors inhibits NSC95397 Cdc25 Phosphatase

Citations for NSC 95397

Citations are publications that use Tocris products.

Currently there are no citations for NSC 95397. Do you know of a great paper that uses NSC 95397 from Tocris? If so please let us know.

Commented out for usability testing

Reviews

TODO: Add Reviews

Literature in this Area

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Pathways for NSC 95397

Protocols

TODO: Add Protocols