Cdc25 Phosphatase

Cdc25 is a dual specificity phosphatase with three isoforms in mammalian cells - Cdc25A, B and C. Cdc25 activates cdk complexes that drive the cell cycle. Cdc25 is involved in the DNA damage checkpoints and is known as a key mediator of cell cycle progression.

Products
Background
Literature
Gene Data

Inhibitors

Cat No Product Name / Activity
1867 NSC 663284
Potent, selective Cdc25 phosphatase inhibitor
1547 NSC 95397
Selective Cdc25 dual specificity phosphatase inhibitor

Cdc25 is a dual specificity phosphatase with three isoforms in mammalian cells - Cdc25A, B and C. Cdc25 activates cdk complexes that control progression through the cell cycle. Cdc25 is also involved in the DNA damage checkpoints.

Regulation of Cdc25 occurs through a variety of mechanisms including phosphorylation, interaction with 14.3.3 proteins, subcellular localization and protein degradation. Cdc25B is phosphorylated and activated by aurora kinase A at the start of mitosis, and as mitosis progresses is then further phosphorylated in an auto-amplification loop, along with Cdc25C, by the CDK1/cyclin B complex. Cdc25C is also phosphorylated during mitosis by PLK1, leading to greater activity of the CDK1/cyclin B complex. Similarly, Cdc25A acts during the G1/S phase of the cell cycle in concert with the CDK2/cyclin E complex. Both Cdc25A and Cdc25B can be activated by the DNA damage checkpoint kinase, Chk1 and thereby inhibit mitosis.

Cdc25 is a key mediator of cell cycle progression. Consequently, there is a strong link between Cdc25 and cancer. Expression of Cdc25A and Cdc25B is upregulated in certain cancer types, frequently those with more aggressive tumors and poor prognosis; the development of specific Cdc25 inhibitors is therefore of great therapeutic interest.

Literature for Cdc25 Phosphatase

Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis
Cell Cycle & DNA Damage Repair

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. Adapted from the 2015 Cancer Product Guide, Edition 3, this poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.

Cdc25 Phosphatase Gene Data

Gene Species Gene Symbol Gene Accession No. Protein Accession No.
Cdc25A Human CDC25A NM_001789 P30304
Mouse Cdc25a NM_007658 P48964
Rat Cdc25a NM_133571 P48965
Cdc25B Human CDC25B NM_021874 P30305
Mouse Cdc25b NM_023117 P30306
Rat Cdc25b NM_133572 P48966
Cdc25C Human CDC25C NM_001790 P30307
Mouse Cdc25c NM_009860 P48967
Rat Cdc25c NM_001107396 NP_001100866