NKH 477

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Description: Water-soluble adenylyl cyclase activator
Alternative Names: Colforsin dapropate hydrochloride,6-(3-dimethylaminopropionyl)forskolin hydrochloride
Chemical Name: N,N-Dimethyl-(3R,4aR,5S,6aS,10S,10aR,10bS)-5-(acetyloxy)-3-ethenyldodecahydro-10,10b-dihydroxy-3,4a,7,7,10a-pentamethyl-1-oxo-1H-naphtho[2,1-b]pyran-6-yl ester β-alanine hydrochloride
Purity: ≥98% (HPLC)
Citations (7)

Biological Activity for NKH 477

NKH 477 is a water-soluble analog of forskolin (Cat. No. 1099) that is a potent activator of adenylyl cyclase; shows some selectivity for cardiac (type V) adenylyl cyclase. Stimulates bronchodilation (EC50 = 32.6 nM) and is an orally active potent positive chronotrope and hypotensive agent in vivo.

Technical Data for NKH 477

M. Wt 546.1
Formula C27H43NO8.HCl
Storage Desiccate at +4°C
Purity ≥98% (HPLC)
CAS Number 138605-00-2
PubChem ID 444028
Smiles O=C(CCN(C)C)O[C@H]2[C@@]1([H])C(C)(C)CC[C@H](O)[C@@](C)1[C@]3(O)[C@@](O[C@](C=C)(C)CC3=O)(C)[C@H]2OC(C)=O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for NKH 477

Solvent Max Conc. mg/mL Max Conc. mM
water 21.84 40

Preparing Stock Solutions for NKH 477

The following data is based on the product molecular weight 546.1. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.4 mM 4.58 mL 22.89 mL 45.78 mL
2 mM 0.92 mL 4.58 mL 9.16 mL
4 mM 0.46 mL 2.29 mL 4.58 mL
20 mM 0.09 mL 0.46 mL 0.92 mL

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Product Datasheets for NKH 477

Certificate of Analysis / Product Datasheet
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References for NKH 477

References are publications that support the biological activity of the product.

Hosono et al (1992) Cardiovascular and adenylate cyclase stimulant properties of NKH477, a novel water-soluble forskolin derivative. J.Cardiovasc.Pharmacol. 19 625 PMID: 1380607

Satake et al (1998) Relaxant effects of NKH477, a new water-soluble forskolin derivative, on guinea-pig tracheal smooth muscle: the role of Ca2+-activated K+ channels. Br.J.Pharmacol. 123 753 PMID: 9517396

Toya et al (1998) Forskolin derivatives with increased selectivity for cardiac adenylyl cyclase. J.Mol.Cell.Cardiol. 30 97 PMID: 9500868

If you know of a relevant reference for NKH 477, please let us know.

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Keywords: NKH 477, NKH 477 supplier, Water-soluble, adenylyl, adenylate, cyclases, activators, cAMP, NKH477, Colforsin, dapropate, hydrochloride, 6-(3-dimethylaminopropionyl)forskolin, Adenylyl, Cyclase, Enzyme, Substrates, /, Activators, 1603, Tocris Bioscience

7 Citations for NKH 477

Citations are publications that use Tocris products. Selected citations for NKH 477 include:

Zhong et al (2012) Phosphodiesterase 4 inhibition impairs cocaine-induced inhibitory synaptic plasticity and conditioned place preference. Neuropsychopharmacology 37 2377 PMID: 22713909

Park et al (2016) Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS. Nat Immunol 17 914 PMID: 27270401

Lee et al (2012) The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP. Nature 492 123 PMID: 23143333

Xia et al (2011) Inhibition of morphine-induced cAMP overshoot: a cell-based assay model in a high-throughput format. PLoS One 31 901 PMID: 21598037

Do you know of a great paper that uses NKH 477 from Tocris? Please let us know.

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