NKH 477

Cat. No. 1603 7 Citations Submit a Review Datasheet / COA / SDS

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Description: Water-soluble adenylyl cyclase activator

Alternative Names: Colforsin dapropate hydrochloride, 6-(3-dimethylaminopropionyl)forskolin hydrochloride

Chemical Name: N,N-Dimethyl-(3R,4aR,5S,6aS,10S,10aR,10bS)-5-(acetyloxy)-3-ethenyldodecahydro-10,10b-dihydroxy-3,4a,7,7,10a-pentamethyl-1-oxo-1H-naphtho[2,1-b]pyran-6-yl ester β-alanine hydrochloride

Purity: ≥98% (HPLC)

Datasheet

Citations (7)

Reviews

Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for NKH 477

NKH 477 is a water-soluble analog of forskolin (Cat. No. 1099) that is a potent activator of adenylyl cyclase; shows some selectivity for cardiac (type V) adenylyl cyclase. Stimulates bronchodilation (EC₅₀ = 32.6 nM) and is an orally active potent positive chronotrope and hypotensive agent in vivo.

Technical Data for NKH 477

M. Wt	546.1
Formula	C₂₇H₄₃NO₈.HCl
Storage	Desiccate at +4°C
Purity	≥98% (HPLC)
CAS Number	138605-00-2
PubChem ID	444028
InChI Key	VIRRLEDAYYYTOD-YHEOSNBFSA-N
Smiles	O=C(CCN(C)C)O[C@H]2[C@@]1([H])C(C)(C)CC[C@H](O)[C@@](C)1[C@]3(O)[C@@](O[C@](C=C)(C)CC3=O)(C)[C@H]2OC(C)=O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for NKH 477

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	21.84	40

Preparing Stock Solutions for NKH 477

The following data is based on the product molecular weight 546.1. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.4 mM	4.58 mL	22.89 mL	45.78 mL
2 mM	0.92 mL	4.58 mL	9.16 mL
4 mM	0.46 mL	2.29 mL	4.58 mL
20 mM	0.09 mL	0.46 mL	0.92 mL

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Product Datasheets for NKH 477

Certificate of Analysis / Product Datasheet

Safety Datasheet

References for NKH 477

References are publications that support the biological activity of the product.

Hosono et al (1992) Cardiovascular and adenylate cyclase stimulant properties of NKH477, a novel water-soluble forskolin derivative. J.Cardiovasc.Pharmacol. 19 625 PMID: 1380607

Satake et al (1998) Relaxant effects of NKH477, a new water-soluble forskolin derivative, on guinea-pig tracheal smooth muscle: the role of Ca²⁺-activated K⁺ channels. Br.J.Pharmacol. 123 753 PMID: 9517396

Toya et al (1998) Forskolin derivatives with increased selectivity for cardiac adenylyl cyclase. J.Mol.Cell.Cardiol. 30 97 PMID: 9500868

If you know of a relevant reference for NKH 477, please let us know.

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