MitoMark Green I

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Cat.No. 6444 - MitoMark Green I | C34H28Cl5N3O | CAS No. 201860-17-5
Description: Green fluorescent mitochondrial stain; cell permeable
Chemical Name: 2-[3-[5,6-Dichloro-1,3-bis[[4-(chloromethyl)phenyl]methyl]-1,3-dihydro-2H-benzimidazol-2-ylidene]-1-propen-1-yl]-3-methylbenzoxazolium chloride
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Citations
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Literature

Biological Activity

Green fluorescent mitochondrial stain. Localizes to mitochondria regardless of mitochondrial membrane potential. Excitation/emission maxima λ ~ 490/516 nm.

MitoMark Green I-labeled HeLa cells

MitoMark

Application of MitoMark Green I: MitoMark Green I was diluted in HBSS and applied to live HeLa cells at a concentration of 200 nM. HeLa cells were incubated for 30 minutes at 37°C. After incubation was complete, MitoMark Green I dilutions were washed off, and fresh HBSS was applied to the cells. Cells were not fixed because MitoMark Green I staining is not retained well after fixation (photobleach). Live cell staining is optimal for MitoMark Green I.

Optical Data for MitoMark Green I

Emission Color Green
λabs 490 nm
λem 516 nm
Extinction Coefficient (ε) 119000 M-1cm-1

Technical Data

M. Wt 671.87
Formula C34H28Cl5N3O
Storage Store at -20°C
CAS Number 201860-17-5
PubChem ID 77913543
InChI Key RUVJFMSQTCEAAB-UHFFFAOYSA-M
Smiles ClC1=CC2=C([N+](CC3=CC=C(CCl)C=C3)=C(/C=C/C=C4N(C)C5=CC=CC=C5O\4)N2CC6=CC=C(CCl)C=C6)C=C1Cl.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 0.67 1

Preparing Stock Solutions

The following data is based on the product molecular weight 671.87. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.01 mM 148.84 mL 744.19 mL 1488.38 mL
0.05 mM 29.77 mL 148.84 mL 297.68 mL
0.1 mM 14.88 mL 74.42 mL 148.84 mL
0.5 mM 2.98 mL 14.88 mL 29.77 mL

Molarity Calculator

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Dilution Calculator

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References

References are publications that support the biological activity of the product.

Garner et al (1997) Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa. Biol.Reprod. 57 1401 PMID: 9408246


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Keywords: MitoMark Green I, MitoMark Green I supplier, MitoMark, green, mitochondrial, marker, stain, mitotracker, mitochondria, Organelle, and, Cell, Structure, Stains, Labels, 6444, Tocris Bioscience

Citations for MitoMark Green I

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Literature in this Area

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