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MitoMark Green I
Biological Activity for MitoMark Green I
MitoMark Green I is a green fluorescent mitochondrial stain. Localizes to mitochondria regardless of mitochondrial membrane potential. Excitation/emission maxima λ ~ 490/516 nm.
It is recommended to prepare stock solutions in DMSO.
Applications in HeLa cells for MitoMark Green I
Application of MitoMark Green I: MitoMark Green I was diluted in HBSS and applied to live HeLa cells at a concentration of 200 nM. HeLa cells were incubated for 30 minutes at 37°C. After incubation was complete, MitoMark Green I dilutions were washed off, and fresh HBSS was applied to the cells. Cells were not fixed because MitoMark Green I staining is not retained well after fixation (photobleach). Live cell staining is optimal for MitoMark Green I.
Optical Data for MitoMark Green I
|Extinction Coefficient (ε)||119000 M-1cm-1|
Technical Data for MitoMark Green I
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for MitoMark Green I
References for MitoMark Green I
References are publications that support the biological activity of the product.
Garner et al (1997) Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa. Biol.Reprod. 57 1401 PMID: 9408246
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Citations for MitoMark Green I
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Literature in this Area
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Fluorescent Probes and Dyes Research Product Guide
This product guide provides a background to the use of Fluorescent Probes and Dyes, as well as a comprehensive list of our
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- Dyes for Flow Cytometry
- Fluorescent Probes
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- Bioluminescent Substrates
- Fluorogenic Dyes for Light-Up Aptamers
- Fluorescent Probes for Imaging Bacteria
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