MitoMark Green I

Discontinued Product

MitoMark Green I (Cat. No. 6444) has been withdrawn from sale for commercial reasons.
Description: Green fluorescent mitochondrial stain; cell permeable
Chemical Name: 2-[3-[5,6-Dichloro-1,3-bis[[4-(chloromethyl)phenyl]methyl]-1,3-dihydro-2H-benzimidazol-2-ylidene]-1-propen-1-yl]-3-methylbenzoxazolium chloride
Purity: ≥85% (HPLC)
Literature (1)

Biological Activity for MitoMark Green I

MitoMark Green I is a green fluorescent mitochondrial stain. Localizes to mitochondria regardless of mitochondrial membrane potential. Excitation/emission maxima λ ~ 490/516 nm.

It is recommended to prepare stock solutions in DMSO.

Scientific Data

Fluorescence Imaging Applications in HeLa cells for MitoMark Green I View Larger

Applications in HeLa cells for MitoMark Green I. Application of MitoMark Green I: MitoMark Green I was diluted in HBSS and applied to live HeLa cells at a concentration of 200 nM. HeLa cells were incubated for 30 minutes at 37°C. After incubation was complete, MitoMark Green I dilutions were washed off, and fresh HBSS was applied to the cells. Cells were not fixed because MitoMark Green I staining is not retained well after fixation (photobleach). Live cell staining is optimal for MitoMark Green I.

Optical Data for MitoMark Green I

Emission Color Green
λabs 490 nm
λem 516 nm
Extinction Coefficient (ε) 119000 M-1cm-1

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

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Technical Data for MitoMark Green I

M. Wt 671.87
Formula C34H28Cl5N3O
Storage Store at -20°C
Purity ≥85% (HPLC)
CAS Number 201860-17-5
PubChem ID 77913543
Smiles ClC1=CC2=C([N+](CC3=CC=C(CCl)C=C3)=C(/C=C/C=C4N(C)C5=CC=CC=C5O\4)N2CC6=CC=C(CCl)C=C6)C=C1Cl.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets for MitoMark Green I

Certificate of Analysis / Product Datasheet
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References for MitoMark Green I

References are publications that support the biological activity of the product.

Garner et al (1997) Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa. Biol.Reprod. 57 1401 PMID: 9408246

Keywords: MitoMark Green I, MitoMark Green I supplier, MitoMark, green, mitochondrial, marker, stain, mitotracker, mitochondria, Organelle, and, Cell, Structure, Stains, Labels, Flow, Cytometry, Other, Mitochondria, Dyes, Probes, 6444, Tocris Bioscience

Citations for MitoMark Green I

Citations are publications that use Tocris products.

Currently there are no citations for MitoMark Green I.

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals