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Description: Potent LIM kinase inhibitor; antitumor
Alternative Names: BMS 5
Chemical Name: N-[5-[1-(2,6-Dichlorophenyl)-3-(difluoromethyl)-1H-pyrazol-5-yl]-2-thiazolyl]-2-methylpropanamide
Purity: ≥98% (HPLC)
Citations (5)
Literature (1)

Biological Activity for LIMKi 3

LIMKi 3 is a potent LIM kinase inhibitor (IC50 values are 7 and 8 nM for LIMK1 and LIMK2 respectively). Inhibits cofilin phosphorylation in MDA-MB-231 breast cancer cells. Reduces MDA-MB-231 tumor cell invasion in a 3D matrigel invasion assay.

Compound Libraries for LIMKi 3

LIMKi 3 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Kinase Inhibitor Library. Find out more about compound libraries available from Tocris.

Technical Data for LIMKi 3

M. Wt 431.29
Formula C17H14Cl2F2N4OS
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 1338247-35-0
PubChem ID 56965901
Smiles FC(F)C1=NN(C2=C(Cl)C=CC=C2Cl)C(C3=CN=C(NC(C(C)C)=O)S3)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for LIMKi 3

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 43.13 100

Preparing Stock Solutions for LIMKi 3

The following data is based on the product molecular weight 431.29. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.32 mL 11.59 mL 23.19 mL
5 mM 0.46 mL 2.32 mL 4.64 mL
10 mM 0.23 mL 1.16 mL 2.32 mL
50 mM 0.05 mL 0.23 mL 0.46 mL

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Product Datasheets for LIMKi 3

Certificate of Analysis / Product Datasheet
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References for LIMKi 3

References are publications that support the biological activity of the product.

Ross-Macdonald et al (2008) Identification of a nonkinase target mediating cytotoxicity of novel kinase inhibitors. Mol.Cancer Ther. 7 3490 PMID: 19001433

Scott et al (2010) LIM kinases are required for invasive path generation by tumor and tumor-associated stromal cells. J.Cell Biol. 191 169 PMID: 20876278

Sparrow et al (2012) The actin-severing protein cofilin is downstream of neuregulin signaling and is essential for Schwann cell myelination. J.Neurosci. 32 5284 PMID: 22496574

If you know of a relevant reference for LIMKi 3, please let us know.

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Keywords: LIMKi 3, LIMKi 3 supplier, LIMKi3, potent, lim, kinases, inhibitors, inhibits, antitumor, BMS5, BMS, 5, LIMK, 4745, Tocris Bioscience

5 Citations for LIMKi 3

Citations are publications that use Tocris products. Selected citations for LIMKi 3 include:

Marie et al (2019) Cyclosporine A inhibits MRTF-SRF signaling through Na+/K+ ATPase inhibition and actin remodeling. FASEB Bioadv 1 561-578 PMID: 32123851

Georgouli et al (2019) Regional Activation of Myosin II in Cancer Cells Drives Tumor Progression via a Secretory Cross-Talk with the Immune Microenvironment. Cell 176 757 PMID: 30712866

Michael R et al (2021) The Wdr1-LIMK-Cofilin Axis Controls B Cell Antigen Receptor-Induced Actin Remodeling and Signaling at the Immune Synapse. Front Cell Dev Biol 9 649433 PMID: 33928084

Johannes et al (2021) Arhgef6 (alpha-PIX) cytoskeletal regulator signals to GTPases and Cofilin to couple T cell migration speed and persistence. J Leukoc Biol 110 839-852 PMID: 33527537

Do you know of a great paper that uses LIMKi 3 from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Cell Cycle & DNA Damage Repair Poster

Cell Cycle & DNA Damage Repair Poster

In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. This poster summarizes the stages of the cell cycle and DNA repair. It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death.