Pricing Availability   Qty
Description: Potent, selective BTK inhibitor
Chemical Name: 2-Cyano-N-(2,5-dibromophenyl)-3-hydroxy-2-butenamide
Purity: ≥98% (HPLC)
Citations (4)

Biological Activity for LFM-A13

LFM-A13 is a potent and selective inhibitor of Bruton's tyrosine kinase (BTK). Inhibits recombinant BTK with an IC50 value of 2.5 μM and has no activity on other protein kinases ( including JAK1, JAK3, HCK, EGFR kinase and insulin receptor kinase) at concentrations of up to 278 μM.

Compound Libraries for LFM-A13

LFM-A13 is also offered as part of the Tocriscreen Kinase Inhibitor Library. Find out more about compound libraries available from Tocris.

Technical Data for LFM-A13

M. Wt 360
Formula C11H8Br2N2O2
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 62004-35-7
PubChem ID 54676905
Smiles C\C(O)=C(/C#N)C(=O)NC1=C(Br)C=CC(Br)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for LFM-A13

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 36 100

Preparing Stock Solutions for LFM-A13

The following data is based on the product molecular weight 360. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.78 mL 13.89 mL 27.78 mL
5 mM 0.56 mL 2.78 mL 5.56 mL
10 mM 0.28 mL 1.39 mL 2.78 mL
50 mM 0.06 mL 0.28 mL 0.56 mL

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Product Datasheets for LFM-A13

Certificate of Analysis / Product Datasheet
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References for LFM-A13

References are publications that support the biological activity of the product.

Crosby and Pool (2002) Interaction of Bruton's tyrosine kinase and protein kinase Cθ in platelets. J.Biol.Chem. 277 9958 PMID: 11788586

Mahajan et al (1999) Rational design and synthesis of a novel anti-leukemic agent targeting Bruton's tyrosine kinase (BTK), LFM-A13 [α-cyano-β-hydroxy-β-methyl-N-(2,5-dibromophenyl)propenamide]. J.Biol.Chem. 274 9587 PMID: 10092645

Vassilev et al (1999) Bruton's tyrosine kinase as an inhibitor of the Fas/CD95 death-inducing signaling complex. J.Biol.Chem. 274 1646 PMID: 9880544

If you know of a relevant reference for LFM-A13, please let us know.

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Keywords: LFM-A13, LFM-A13 supplier, Potent, selective, BTK, inhibitors, inhibits, Brutons, Tyrosine, Kinases, LFMA13, Kinase, (BTK), 1300, Tocris Bioscience

4 Citations for LFM-A13

Citations are publications that use Tocris products. Selected citations for LFM-A13 include:

Tankiewicz-Kwedlo et al (2018) Erythropoietin Intensifies the Proapoptotic Activity of LFM-A13 in Cells and in a Mouse Model of Colorectal Cancer. Int J Mol Sci 19 PMID: 29690619

Colmers and Bains (2018) Balancing tonic and phasic inhibition in hypothalamic CRH-releasing hormone neurons. J Physiol 596 1919 PMID: 29419884

He et al (2016) NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature 530 354 PMID: 26814970

Hart et al (2011) Essential role of Stat3 in PI3K-induced oncogenic transformation. Pain 108 13247 PMID: 21788516

Do you know of a great paper that uses LFM-A13 from Tocris? Please let us know.

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