JW 74

Pricing Availability   Qty
Cat.No. 5653 - JW 74 | C24H20N6O2S | CAS No. 863405-60-1
Description: Wnt pathway inhibitor
Chemical Name: 4-[4-(4-Methoxyphenyl)-5-[[[3-(4-methylphenyl)-1,2,4-oxadiazol-5-yl]methyl]thio]-4H-1,2,4-triazol-3-yl]-pyridine
Purity: ≥99% (HPLC)
Datasheet
Citations (1)
Reviews
Literature
Pathways

Biological Activity

Inhibitor of canonical Wnt signaling (IC50 = 790 nM). Targets the β-catenin destruction complex (GSK-3 beta /AXIN/APC). Increases AXIN2 and decreases active β-catenin levels in colorectal cancer (CRC) cells. Also arrests cell cycle in G1 and inhibits proliferation of CRC cells in vitro. Inhibits tumor growth and formation in mouse models.

Compound Libraries

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Technical Data

M. Wt 456.52
Formula C24H20N6O2S
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 863405-60-1
PubChem ID 16013152
InChI Key KRIKILRRJCIWNB-UHFFFAOYSA-N
Smiles COC1=CC=C(N2C(SCC3=NC(C4=CC=C(C)C=C4)=NO3)=NN=C2C5=CC=NC=C5)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 45.65 100

Preparing Stock Solutions

The following data is based on the product molecular weight 456.52. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.19 mL 10.95 mL 21.9 mL
5 mM 0.44 mL 2.19 mL 4.38 mL
10 mM 0.22 mL 1.1 mL 2.19 mL
50 mM 0.04 mL 0.22 mL 0.44 mL

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References

References are publications that support the biological activity of the product.

Waaler et al (2011) Novel synthetic antagonists of canonical Wnt signaling inhibit colorectal cancer cell growth. Cancer Res. 71 197 PMID: 21199802


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Keywords: JW 74, JW 74 supplier, JW74, canonical, Wnt, signaling, pathway, inhibitors, inhibits, cancer, β-catenin, AXIN2, Beta-catenin, Other, Signaling, 5653, Tocris Bioscience

1 Citation for JW 74

Citations are publications that use Tocris products. Selected citations for JW 74 include:

Rothberg (2018) Mtf2-PRC2 control of canonical Wnt signaling is required for definitive erythropoiesis. Cell Discov 4 21 PMID: 29736258


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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

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Cancer

Cancer Research Product Guide

A collection of over 750 products for cancer research, the guide includes research tools for the study of:

  • Cancer Metabolism
  • Epigenetics in Cancer
  • Receptor Signaling
  • Cell Cycle and DNA Damage Repair
  • Angiogenesis
  • Invasion and Metastasis

Pathways for JW 74

Wnt

Wnt Signaling Pathway

The Wnt pathway is involved in cellular differentiation and proliferation in adult tissues and also during embryogenesis. Disturbances within the pathway may lead to the formation of tumors and promote metastasis.