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Submit ReviewJanelia Fluor® 585, SE is an orange fluorogenic fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 585 nm; emission maximum = 609 nm; Quantum yield = 0.78; Max extinction coefficient = 156,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); Correction factor = 0.0990. Exhibits low non-specific background staining.
Please see the protocol for further information on protein/antibody labeling and conjugation.
To measure the absorbance spectrum of this dye we recommend the following solvent: trifluoroethanol plus 0.1% TFA.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Emission Color | Orange |
---|---|
Brightness | |
λabs | 585 nm |
λem | 609 nm |
Extinction Coefficient (ε) | 156,000 M-1cm-1 |
Quantum Yield (φ) | 0.78 |
Closest Laser line | 561 nm |
Reactive Group | NHS ester |
Reactivity | Primary amines |
Cell Permeable | Yes |
Correction Factor 280 | 0.099 |
Application | Confocal Imaging, SRM, Two-photon Excitation |
Alternative For | Alexa Fluor® 568, Atto Rho12, Atto Rho101, CF594 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral ViewerM. Wt | 649.6 |
Formula | C34H27F4N3O6 |
Storage | Store at -20°C |
Purity | ≥95% (HPLC) |
CAS Number | 1811539-88-4 |
PubChem ID | 132470913 |
InChI Key | GFNMIANIZWSVQR-UHFFFAOYSA-N |
Smiles | O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)(F)C4)C=C3C5(C)C)=C(C=C/6)C5=CC6=[N+]7CC(F)(F)C\7)=C2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solvent | Max Conc. mg/mL | Max Conc. mM | |
---|---|---|---|
Solubility | |||
DMSO | 64.96 | 100 |
The following data is based on the product molecular weight 649.6. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.54 mL | 7.7 mL | 15.39 mL |
5 mM | 0.31 mL | 1.54 mL | 3.08 mL |
10 mM | 0.15 mL | 0.77 mL | 1.54 mL |
50 mM | 0.03 mL | 0.15 mL | 0.31 mL |
References are publications that support the biological activity of the product.
Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID: 28869757
If you know of a relevant reference for Janelia Fluor® 585, SE, please let us know.
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The following protocol features additional information for the use of Janelia Fluor® 585, SE (Cat. No. 6418).
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our: