Janelia Fluor® 585, SE

Pricing Availability   Qty
Cat.No. 6418 - Janelia Fluor 585, SE | C34H27F4N3O6 | CAS No. 1811539-88-4
Description: Orange dye (Reactive group: NHS ester). Applications: super resolution microscopy, confocal, two-photon excitation and live cell imaging
Alternative Names: JF 585, NHS
Chemical Name: 1-[10-[2-Carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-7-(3,3-difluoro-1-azetidinyl)-9,9-dimethyl-2(9H)-anthracenylidene]-3,3-difluoroazetidinium, inner salt
Protocols (1)
Literature (1)

Biological Activity

Orange fluorogenic fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 585 nm; emission maximum = 609 nm; Quantum yield = 0.78; Max extinction coefficient = 156,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); Correction factor = 0.0990. Exhibits low non-specific background staining.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor® 585, SE

Janelia Fluor<sup>®</sup> 585, SE Dye Spectra
Emission Color Orange
λabs 585 nm
λem 609 nm
Extinction Coefficient (ε) 156,000 M-1cm-1
Quantum Yield (φ) 0.78
Closest Laser line 561 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Correction Factor 280 0.099
Application Confocal imaging, SRM, Two-photon excitation
Alternative For Alexa Fluor® 568, Atto Rho12, Atto Rho101, CF594

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Technical Data

M. Wt 649.6
Formula C34H27F4N3O6
Storage Store at -20°C
CAS Number 1811539-88-4
PubChem ID 132470913
Smiles O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)(F)C4)C=C3C5(C)C)=C(C=C/6)C5=CC6=[N+]7CC(F)(F)C\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 64.96 100

Preparing Stock Solutions

The following data is based on the product molecular weight 649.6. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.54 mL 7.7 mL 15.39 mL
5 mM 0.31 mL 1.54 mL 3.08 mL
10 mM 0.15 mL 0.77 mL 1.54 mL
50 mM 0.03 mL 0.15 mL 0.31 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.


References are publications that support the biological activity of the product.

Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID: 28869757

If you know of a relevant reference for Janelia Fluor® 585, SE, please let us know.

Keywords: Janelia Fluor 585, SE, Janelia Fluor 585, SE supplier, JF585, Janelia, Fluor, 585-SE, fluorescent, dyes, NHS, ester, self-labeling, tag, systems, microscopy, cell, permeable, super, resolution, JF, 585,, Amine, Reactive, Dyes, Flow, Cytometry, 6418, Tocris Bioscience

Citations for Janelia Fluor® 585, SE

Citations are publications that use Tocris products.

Currently there are no citations for Janelia Fluor® 585, SE. Do you know of a great paper that uses Janelia Fluor® 585, SE from Tocris? Please let us know.

Reviews for Janelia Fluor® 585, SE

There are currently no reviews for this product. Be the first to review Janelia Fluor® 585, SE and earn rewards!

Have you used Janelia Fluor® 585, SE?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Protocols for Janelia Fluor® 585, SE

The following protocol features additional information for the use of Janelia Fluor® 585, SE (Cat. No. 6418).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* or download your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

New Product Guide

New Product Guide [Spring/Summer 2019]

Our new product guide highlights over 215 new products added to the Tocris Bioscience range during the first half of 2019.

  • 7-TM Receptors
  • Enzymes
  • Enzyme-Linked Receptors
  • Ion Channels
  • Nuclear Receptors
  • Transporters
  • Chemogenetics
  • Epigenetics
  • Fluorescent Imaging
  • Signal Transduction
  • Stem Cells