Janelia Fluor® 585, SE

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Description: Orange dye (Reactive group: NHS ester). Application: confocal microscopy, super resolution microscopy, and two-photon excitation fluorescence microscopy. Suitable for live cell imaging
Alternative Names: JF 585,NHS
Chemical Name: 1-[10-[2-Carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-7-(3,3-difluoro-1-azetidinyl)-9,9-dimethyl-2(9H)-anthracenylidene]-3,3-difluoroazetidinium, inner salt
Purity: ≥95% (HPLC)
Protocols (1)
Literature (1)

Biological Activity for Janelia Fluor® 585, SE

Key Information: Janelia Fluor® 585, SE is an orange fluorogenic fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.

Applications: Suitable for confocal microscopy, two-photon excitation fluorescence microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Cell permeable.

Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 585 nm and 609 nm, respectively; quantum yield = 0.78; extinction coefficient = 156,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); A280 correction factor = 0.099; lactone-zwitterion equilibrium constant (KL-Z) < 0.0001. Exhibits low non-specific background staining.

Please see the protocol for further information on protein/antibody labeling and conjugation.

To measure the absorbance spectrum of this dye we recommend the following solvent: trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor® 585, SE

Janelia Fluor<sup>®</sup> 585, SE Dye Spectra
Emission Color Orange
λabs 585 nm
λem 609 nm
Extinction Coefficient (ε) 156,000 M-1cm-1
Quantum Yield (φ) 0.78
Closest Laser line 561 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable Yes
Correction Factor 280 0.099
Application Confocal Microscopy, SRM, Two-photon Excitation Fluorescence Microscopy
Alternative For Alexa Fluor® 568, Atto Rho12, Atto Rho101, CF594

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for Janelia Fluor® 585, SE

M. Wt 649.6
Formula C34H27F4N3O6
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 1811539-88-4
PubChem ID 132470913
Smiles O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)(F)C4)C=C3C5(C)C)=C(C=C/6)C5=CC6=[N+]7CC(F)(F)C\7)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Janelia Fluor® 585, SE

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 64.96 100

Preparing Stock Solutions for Janelia Fluor® 585, SE

The following data is based on the product molecular weight 649.6. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.54 mL 7.7 mL 15.39 mL
5 mM 0.31 mL 1.54 mL 3.08 mL
10 mM 0.15 mL 0.77 mL 1.54 mL
50 mM 0.03 mL 0.15 mL 0.31 mL

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Product Datasheets for Janelia Fluor® 585, SE

Certificate of Analysis / Product Datasheet
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References for Janelia Fluor® 585, SE

References are publications that support the biological activity of the product.

Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID: 28869757

Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787

If you know of a relevant reference for Janelia Fluor® 585, SE, please let us know.

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Citations for Janelia Fluor® 585, SE

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Protocols for Janelia Fluor® 585, SE

The following protocol features additional information for the use of Janelia Fluor® 585, SE (Cat. No. 6418).

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

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