Janelia Fluor® 585, SENew
Orange fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 585 nm; emission maximum = 609 nm; Quantum yield = 0.78; Max extinction coefficient = 156,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); Correction factor = 0.0990. Exhibits low non-specific background staining.
To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 649.6. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.54 mL||7.7 mL||15.39 mL|
|5 mM||0.31 mL||1.54 mL||3.08 mL|
|10 mM||0.15 mL||0.77 mL||1.54 mL|
|50 mM||0.03 mL||0.15 mL||0.31 mL|
References are publications that support the products' biological activity.
Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID: 28869757
If you know of a relevant reference for Janelia Fluor® 585, SE, please let us know.
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Protocols for Janelia Fluor® 585, SE
The following protocol features additional information for the use of Janelia Fluor® 585, SE (Cat. No. 6418).
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