Maintains embryonic stem cell (ESC) pluripotency in Wnt3a-treated cells; enables Wnt/β-catenin-driven expansion of mouse ESCs and prevents spontaneous differentiation. Upregulates expression of Oct4 and Sox2 transcription factors. Binds to serine/threonine phosphatase PP2A and prevents PP2A/Nkd interaction.
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 362.42. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||2.76 mL||13.8 mL||27.59 mL|
|5 mM||0.55 mL||2.76 mL||5.52 mL|
|10 mM||0.28 mL||1.38 mL||2.76 mL|
|50 mM||0.06 mL||0.28 mL||0.55 mL|
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
References are publications that support the products' biological activity.
Miyabayashi et al (2007) Wnt/beta-catenin/CBP signaling maintains long-term murine embryonic stem cell pluripotency. Proc.Natl.Acad.Sci.U S A 104 5668 PMID: 17372190
If you know of a relevant reference for IQ 1, please let us know.
Keywords: IQ1 embryonic stem cells ESCs pluripotency dedifferentiation maintains cultured Wnt3a blocks differentiation PP2A Nkd Oct4 Sox2 Stem Cell Proliferation
1 Citation for IQ 1
Citations are publications that use Tocris products. Selected citations for IQ 1 include:
Long et al (2016) Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin. J Neurosci 7 10354 PMID: 26838601
Do you know of a great paper that uses IQ 1 from Tocris? If so please let us know.
Literature in this Area
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.