IPA 3

Pricing Availability   Qty
Description: Group I PAK inhibitor
Chemical Name: 1,1'-Dithiodi-2-naphthtol
Purity: ≥96% (HPLC)
Datasheet
Citations (32)
Reviews

Biological Activity for IPA 3

IPA 3 is a group I p21-activated kinase (PAK) inhibitor (IC50 = 2.5 μM at PAK1). Targets the autoregulatory mechanism and promotes the inactive conformation of PAKs. Inhibits PAK1-mediated signaling in vivo; potential anti-tumor agent.

Negative Control also available.

Technical Data for IPA 3

M. Wt 350.45
Formula C20H14O2S2
Storage Store at -20°C
Purity ≥96% (HPLC)
CAS Number 42521-82-4
PubChem ID 521106
InChI Key RFAXLXKIAKIUDT-UHFFFAOYSA-N
Smiles OC2=C(SSC3=C(O)C=CC4=C3C=CC=C4)C1=CC=CC=C1C=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for IPA 3

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 26.28 75

Preparing Stock Solutions for IPA 3

The following data is based on the product molecular weight 350.45. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.75 mM 3.8 mL 19.02 mL 38.05 mL
3.75 mM 0.76 mL 3.8 mL 7.61 mL
7.5 mM 0.38 mL 1.9 mL 3.8 mL
37.5 mM 0.08 mL 0.38 mL 0.76 mL

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Product Datasheets for IPA 3

Certificate of Analysis / Product Datasheet
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References for IPA 3

References are publications that support the biological activity of the product.

Kumar et al (2006) p21-activated kinases in cancer. Nat.Rev.Cancer 6 459 PMID: 16723992

Deacon et al (2008) An isoform-selective, small-molecule inhibitor targets the autoregulatory mechanism of p21-activated kinase. Chem.Biol. 15 322 PMID: 18420139

Takahashi and Suzuki (2009) Membrane transport of WAVE2 and lamellipodia formation require Pak1 that mediates phosphorylation and recruitment of stathmin/op18 to Pak1-WAVE2-kinesin complex. Cell.Signal. 21 695 PMID: 19162178

Viaud and Peterson (2009) An allosteric kinase inhibitor binds the p21-activated kinase (Pak) autoregulatory domain covalently. Mol.Cancer Ther. 8 2559 PMID: 19723886

Wang et al (2016) P21-activated kinase inhibitors FRAX486 and IPA3: inhibition of prostate stromal cell growth and effects on smooth muscle contraction in the human prostate. PLoS One 11 e0153312 PMID: 27071060


If you know of a relevant reference for IPA 3, please let us know.

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Keywords: IPA 3, IPA 3 supplier, Group, I, p21-activated, kinases, PAK, PAK1, inhibitors, inhibits, IPA3, P21-activated, Kinases, 3622, Tocris Bioscience

32 Citations for IPA 3

Citations are publications that use Tocris products. Selected citations for IPA 3 include:

Das et al (2017) Affecting long-term fear memory formation through optical control of Rac1 GTPase and PAK activity in lateral amygdala. Sci Rep 7 13930 PMID: 29066727

Berger et al (2014) PAK-dependent STAT5 serine phosphorylation is required for BCR-ABL-induced leukemogenesis. Invest Ophthalmol Vis Sci 28 629 PMID: 24263804

Molosh et al (2014) Social learning and amygdala disruptions in Nf1 mice are rescued by blocking p21-activated kinase. Int J Cancer Clin Res 17 1583 PMID: 25242307

Al-Ejeh et al (2014) Kinome profiling reveals breast cancer heterogeneity and identifies targeted therapeutic opportunities for triple negative breast cancer. J Pharmacol Exp Ther 5 3145 PMID: 24762669

Dipaolo et al (2013) Rac1 pathway mediates stretch response in pulmonary alveolar epithelial cells. Nat Commun 305 L141 PMID: 23686855

Wang and Townes-Anderson (2015) LIM Kinase, a Newly Identified Regulator of Presynaptic Remodeling by Rod Photoreceptors After Injury. Nat Neurosci 56 7847 PMID: 26658506

Durkin et al (2017) RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6. Dev Cell 41 315 PMID: 28486133

Pandolfi et al (2015) PAK1 is a therapeutic target in acute myeloid leukemia and myelodysplastic syndrome. Blood 126 1118 PMID: 26170031

Imle et al (2015) Association with PAK2 Enables Functional Interactions of Lentiviral Nef Proteins with the Exocyst Complex. MBio 6 PMID: 26350970

Reinardy et al (2015) Phosphorylation of Threonine 794 on Tie1 by Rac1/PAK1 Reveals a Novel Angiogenesis Regulatory Pathway. PLoS One 10 e0139614 PMID: 26436659

Mortazavi et al (2015) Significance of KRAS/PAK1/Crk pathway in non-small cell lung cancer oncogenesis. BMC Cancer 15 381 PMID: 25956913

Huttunen et al (2014) Coxsackievirus A9 infects cells via nonacidic multivesicular bodies. J Virol 88 5138 PMID: 24574401

Brenda M et al (2021) Kinases of the Focal Adhesion Complex Contribute to Cardiomyocyte Specification. Int J Mol Sci 22 PMID: 34638793

Petra et al (2021) Group I p21-activated kinases in leukemia cell adhesion to fibronectin. Cell Adh Migr 15 18-36 PMID: 33464167

Li et al (2021) Activation of Rac1 Has an Opposing Effect on Induction and Maintenance of Long-Term Potentiation in Hippocampus by Acting on Different Kinases. Front Mol Neurosci 14 720371 PMID: 34531724

Martin et al (2016) PAK proteins and YAP-1 signalling downstream of integrin beta-1 in myofibroblasts promote liver fibrosis. Nat Commun 7 12502 PMID: 27535340

Ji et al (2013) Biphasic regulation of myosin light chain phosphorylation by p21-activated kinase modulates intestinal smooth muscle contractility. J Biol Chem 288 1200-13 PMID: 23161543

Payaningal R et al (2020) PAK1 inhibitor IPA-3 mitigates metastatic prostate cancer-induced bone remodeling. Biochem Pharmacol 177 113943 PMID: 32240651

Marc et al (2020) Interrogation of kinase genetic interactions provides a global view of PAK1-mediated signal transduction pathways. J Biol Chem 295 16906-16919 PMID: 33453946

Eckart D et al (2020) CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles. Cell Rep 30 2444-2459.e7 PMID: 32075774

Di et al (2020) Macropinocytosis drives T cell growth by sustaining the activation of mTORC1. Nat Commun 11 180 PMID: 31924779

Payaningal R et al (2020) Sterically stabilized liposomes targeting P21 (RAC1) activated kinase-1 and secreted phospholipase A2 suppress prostate cancer growth and metastasis. Oncol Lett 20 179 PMID: 32934746

Marc et al (2020) Interrogation of kinase genetic interactions provides a global view of PAK1-mediated signal transduction pathways. J Biol Chem 295 16906-16919 PMID: 33060198

Amit et al (2019) PAK Kinase Inhibition Has Therapeutic Activity in Novel Preclinical Models of Adult T-Cell Leukemia/Lymphoma. Clin Cancer Res 25 3589-3601 PMID: 30862694

Zeng et al (2016) 1α,25(OH)2D3 Induces Actin Depolymerization in Endometrial Carcinoma Cells by Targeting RAC1 and PAK1. Cell Physiol Biochem 40 1455 PMID: 27997893

Wu et al (2010) Pak1 and Pak2 are activated in recurrent respiratory papillomas, contributing to one pathway of Rac1-mediated COX-2 expression. Int J Cancer 127 2230 PMID: 20131316

Zhang et al (2018) Long-Term In Vitro Expansion of Epithelial Stem Cells Enabled by Pharmacological Inhibition of PAK1-ROCK-Myosin II and TGF-β Signaling. Cell Rep 25 598 PMID: 30332641

Wang et al (2016) P21-activated kinase inhibitors FRAX486 and IPA3: inhibition of prostate stromal cell growth and effects on smooth muscle contraction in the human prostate. PLoS One 11 e0153312 PMID: 27071060

Salker et al (2016) LeftyA decreases Actin Polymerization and Stiffness in Human Endometrial Cancer Cells Scientific Reports 6 29370 PMID: 27404958

Oladimeji et al (2015) A Derivative of Differentiation-Inducing Factor-3 Inhibits PAK1 Activity and Breast Cancer Cell Proliferation. Am J Physiol Lung Cell Mol Physiol 2 42522 PMID: 26688830

Sorce et al (2015) Mitotic cells contract actomyosin cortex and generate pressure to round against or escape epithelial confinement. Oncotarget 6 8872 PMID: 26602832

Martin et al (2013) Pak and Rac GTPases promote oncogenic KIT-induced neoplasms. J Clin Invest 123 4449 PMID: 24091327


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