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Ionomycin free acid
|Storage||Desiccate at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
Preparing Stock Solutions
The following data is based on the product molecular weight 709.01. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.1 mM||14.1 mL||70.52 mL||141.04 mL|
|0.5 mM||2.82 mL||14.1 mL||28.21 mL|
|1 mM||1.41 mL||7.05 mL||14.1 mL|
|5 mM||0.28 mL||1.41 mL||2.82 mL|
References are publications that support the biological activity of the product.
Liu and Hermann (1978) Characterization of ionomycin as a calcium ionophore. J.Biol.Chem. 253 5892 PMID: 28319
Kaufmann et al (1980) Cation transport and specificity of ionomycin. Comparison with ionophore A23187 in rat liver mitochondria. J.Biol.Chem. 255 2735 PMID: 6766939
Elzi et al (2001) Ionomycin causes activation of p38 and p42/44 mitogen-activated protein kinases in human neutrophils. Am.J.Physiol.Cell Physiol. 281 C350 PMID: 11401859
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Keywords: Ionomycin free acid, Ionomycin free acid supplier, Calcium, ionophore, Signaling, Signalling, Agents, General, Ca2+, Ionophore, Ionophores, Antibiotics, 2092, Tocris Bioscience
1 Citation for Ionomycin free acid
Citations are publications that use Tocris products. Selected citations for Ionomycin free acid include:
Mestre et al (2019) Manipulation of Gut Microbiota Influences Immune Responses, Axon Preservation, and Motor Disability in a Model of Progressive Multiple Sclerosis. Front Immunol 10 1374 PMID: 31258540
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Average Rating: 4.5 (Based on 2 Reviews.)
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HEK293 cells were loaded with 1 µM intracellular Ca2+ sensitive dye, Fura-2AM in normal tyrode solution for 30 min in dark, washed, and left to equilibrate for 15 min. Fura2-loaded cells were excited alternately at 340 and 380 nm and the fluorescence at 510 nm was expressed as 340/380 ratio to indicate intracellular Ca2+ levels. Application of 1 µM ionomycin in Ca2+-free tyrode solution released Ca2+ from intracellular stores, manifested as a transient peak in fluorescence ratio.
Ionomycin was used as a calcium ionophore to bring large amounts of extracellular Ca2+ into activated CD8+ T-cells. With high intracellular Ca2+, this led to increased activation of CD8+ T-cells as measured by IFNg production via intracellular flow cytometry.