A23187, free acid

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Cat.No. 1234 - A23187, free acid | C29H37N3O6 | CAS No. 52665-69-7
Description: Calcium ionophore
Alternative Names: Calcimycin
Chemical Name: 5-(Methylamino)-2-[[2R,3R,6S,8S,9R,11R)-3,9,11-trimethyl-8-[(1S)-1-methyl-2-oxo-2-(1H-pyrrol-2-yl)-ethyl]-1,7-dioxaspiro[5.5]undec-2-yl]methyl]-4-benzoxazolecarboxylic acid
Purity: ≥98% (HPLC)
Datasheet
Citations (11)
Reviews (1)

Biological Activity

Calcium ionophore that induces Ca2+-dependent cell death by increasing intracellular calcium concentration. Promotes intracelllular ROS generation and platelet particle formation (fragmentation) in vitro and in vivo. Can be used to induce autophagy in mammalian cells.

Technical Data

M. Wt 523.63
Formula C29H37N3O6
Storage Store at +4°C
Purity ≥98% (HPLC)
CAS Number 52665-69-7
PubChem ID 11957499
InChI Key HIYAVKIYRIFSCZ-CYEMHPAKSA-N
Smiles [H][C@@]1([C@H](C)C(C5=CC=CN5)=O)[C@H](C)C[C@@H](C)[C@@]2(O[C@H](CC3=NC(C(C(O)=O)=C(NC)C=C4)=C4O3)[C@H](C)CC2)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 26.18 50

Preparing Stock Solutions

The following data is based on the product molecular weight 523.63. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 3.82 mL 19.1 mL 38.19 mL
2.5 mM 0.76 mL 3.82 mL 7.64 mL
5 mM 0.38 mL 1.91 mL 3.82 mL
25 mM 0.08 mL 0.38 mL 0.76 mL

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Product Datasheets

Certificate of Analysis / Product Datasheet
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Keywords: A23187, free acid, A23187, free acid supplier, Calcium, ionophore, Ca2+, Ionophore, Signaling, Signalling, Agents, General, antibiotics, Calcimycin, Ionophores, Antibiotics, Autophagy, 1234, Tocris Bioscience

11 Citations for A23187, free acid

Citations are publications that use Tocris products. Selected citations for A23187, free acid include:

Cheang et al (2011) Endothelial nitric oxide synthase enhancer reduces oxidative stress and restores endothelial function in db/db mice. Cell Death Dis 92 267 PMID: 21875904

Wajih et al (2017) Potential therapeutic action of nitrite in sickle cell disease. Redox Biol 12 1026 PMID: 28511346

Schöndorf et al (2014) iPSC-derived neurons from GBA1-associated Parkinson's disease patients show autophagic defects and impaired calcium homeostasis. Nat Commun 5 4028 PMID: 24905578

Cahalan et al (2015) Piezo1 links mechanical forces to red blood cell volume. Cardiovasc Res 4 PMID: 26001274


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Reviews for A23187, free acid

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The A23187-induced cytotoxicity cytometrically examined in rat thymocytes.
By Lutpine Rachel on 01/02/2020
Assay Type: In Vitro
Species: Human

A23187 is used to induce Ca(2+)-dependent cell death by increasing intracellular Ca(2+) concentration ([Ca(2+)](i)) under in vitro condition. Since this ionophore also increases membrane permeability of metal divalent cations such as Zn(2+) and Fe(2+) rather than Ca(2+), trace metal cations in cell suspension may affect Ca(2+)-dependent cell death induced by A23187. Therefore, the effects of chelators for divalent metal cations, EDTA and TPEN, on the A23187-induced cytotoxicity were cytometrically examined in rat thymocytes.

The cytotoxicity of A23187 was attenuated by 1mM EDTA while it was augmented by 50 microM EDTA and 10 microM TPEN.

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