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Indocyanine green is a near infrared (NIR) fluorescent dye operating in the 700-900 nm wavelength range. It also exhibits fluorescence emission in the shortwave infrared window (SWIR; 1,000-2,000 nm) which enables in vivo imaging, including intravital microscopy, non-invasive real-time imaging in blood and lymph vessels, and imaging of hepatobiliary clearance. Imaging of indocyanine green in SWIR shows increased sensitivity, contrast, and resolution of fine anatomical structures over NIR.
This product is supplied with a high degree of hydration and may contain residual inorganic salts, the amount of which are batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.Spectral Viewer
|Storage||Store at -20°C|
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
|Solvent||Max Conc. mg/mL||Max Conc. mM|
The following data is based on the product molecular weight 774.97. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|0.5 mM||2.58 mL||12.9 mL||25.81 mL|
|2.5 mM||0.52 mL||2.58 mL||5.16 mL|
|5 mM||0.26 mL||1.29 mL||2.58 mL|
|25 mM||0.05 mL||0.26 mL||0.52 mL|
References are publications that support the biological activity of the product.
Starosolski et al (2017) Indocyanine green fluorescence in second near-infrared (NIR-II) window. PLoS One 12 e0187563 PMID: 29121078
Carr et al (2018) Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green. Proc.Natl.Acad.Sci.U.S.A. 115 4465 PMID: 29626132
Cosco et al (2021) Photophysical properties of indocyanine green in the shortwave infrared region. ChemPhotoChem. 5 727 PMID: 34504949
Lee et al (2021) Surgical outcomes of localization using indocyanine green fluorescence in breast conserving surgery: a prospective study. Sci.Rep. 11 9997 PMID: 33976314
Li et al (2019) Recent progress in small-molecule near-IR probes for bioimaging. Trends Chem. 1 224 PMID: 32864595
If you know of a relevant reference for Indocyanine green, please let us know.
Keywords: Indocyanine green, Indocyanine green supplier, NIR, near, infrared, short, wave, SWIR, fluorescent, dye, fluorescence, real, time, imaging, labeling, labelling, ICG, Cyanine, Dyes, (Cy, Dyes), Near, Infrared, (NIR), Fluorescent, 7510, Tocris Bioscience
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This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our: