Indocyanine green

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Description: Near-infrared (NIR) fluorescent dye; suitable for in vivo imaging
Alternative Names: ICG
Chemical Name: Sodium 4-(2-((1E,3E,5E,7Z)-7-(1,1-Dimethyl-3-(4-sulfonatobutyl)-1,3-dihydro-2H-benzo[e]indol-2-ylidene)hepta-1,3,5-trien-1-yl)-1,1-dimethyl-1H-benzo[e]indol-3-ium-3-yl)butane-1-sulfonate
Purity: ≥95% (HPLC)
Literature (1)

Biological Activity for Indocyanine green

Key information: Indocyanine green (ICG) is a near-infrared (NIR) fluorescent dye suitable for in vivo imaging.

Application: Fluorescence microscopy, in vivo imaging.

Properties and Photophysical Data: Indocyanine green is a NIR fluorescent dye operating in the 700-900 nm wavelength range. It binds to β-lipoproteins, particularly albumin, in plasma and exhibits fluorescence emission in the shortwave infrared window (SWIR; 1,000-2,000 nm) which enables in vivo imaging, including intravital microscopy, non-invasive real-time imaging in blood and lymph vessels, and imaging of hepatobiliary clearance. Imaging of indocyanine green in SWIR shows increased sensitivity, contrast, and resolution of fine anatomical structures over NIR. Excitation and emission maxima (λ) are 787 nm and 815 nm, respectively; quantum yield = 0.14; extinction coefficient = 223,000 M-1cm-1; A280 correction factor = 0.07.

This product is supplied with a high degree of hydration and may contain residual inorganic salts, the amount of which are batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.

Optical Data for Indocyanine green

Indocyanine green Dye Spectra
Emission Color Near-IR
λabs 787 nm
λem 815 nm
Extinction Coefficient (ε) 223000 M-1cm-1
Quantum Yield (φ) 0.14
Closest Laser line 785 nm
pH Sensitivity No
Correction Factor 280 0.07
Application Fluorescence Microscopy, in vivo Imaging

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Technical Data for Indocyanine green

M. Wt 774.97
Formula C43H47N2O6S2Na
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 3599-32-4
PubChem ID 45101199
Smiles CC1(/C(N(C2=C1C3=CC=CC=C3C=C2)CCCCS(=O)(O[Na])=O)=C/C=C/C=C/C=C/C4=[N+](C5=C(C4(C)C)C6=CC=CC=C6C=C5)CCCCS([O-])(=O)=O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Indocyanine green

Solvent Max Conc. mg/mL Max Conc. mM
DMSO 15.5 20
water 38.75 50

Preparing Stock Solutions for Indocyanine green

The following data is based on the product molecular weight 774.97. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 2.58 mL 12.9 mL 25.81 mL
2.5 mM 0.52 mL 2.58 mL 5.16 mL
5 mM 0.26 mL 1.29 mL 2.58 mL
25 mM 0.05 mL 0.26 mL 0.52 mL

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Product Datasheets for Indocyanine green

References for Indocyanine green

References are publications that support the biological activity of the product.

Starosolski et al (2017) Indocyanine green fluorescence in second near-infrared (NIR-II) window. PLoS One 12 e0187563 PMID: 29121078

Carr et al (2018) Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green. Proc.Natl.Acad.Sci.U.S.A. 115 4465 PMID: 29626132

Cosco et al (2021) Photophysical properties of indocyanine green in the shortwave infrared region. ChemPhotoChem. 5 727 PMID: 34504949

Lee et al (2021) Surgical outcomes of localization using indocyanine green fluorescence in breast conserving surgery: a prospective study. Sci.Rep. 11 9997 PMID: 33976314

Li et al (2019) Recent progress in small-molecule near-IR probes for bioimaging. Trends Chem. 1 224 PMID: 32864595

If you know of a relevant reference for Indocyanine green, please let us know.

Keywords: Indocyanine green, Indocyanine green supplier, NIR, near, infrared, short, wave, SWIR, fluorescent, dye, fluorescence, real, time, imaging, labeling, labelling, ICG, Cyanine, Dyes, (Cy, Dyes), Near, Infrared, (NIR), Fluorescent, 7510, Tocris Bioscience

Citations for Indocyanine green

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